FastQCFastQC Report
Mon 6 Jun 2016
ERR1211653_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211653_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6727818
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234630.34874605704256567No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201750.2998743426174727No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142900.21240170289981092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102950.051.8387681
GTATCAA181350.039.410891
TCAACGC228850.030.3283024
ATCAACG230900.030.1205393
CAACGCA230900.030.0382395
TACCTGG58650.029.5080552
GTACCTG62700.029.0238041
AACGCAG241150.028.8985216
TATCAAC243300.028.8008272
GTACATG221700.028.4102691
TAACGCA7000.028.1948474
TATAACG6850.028.1484222
TACATGG226400.027.1493822
ACATGGG226400.026.277393
CATGGGG154900.025.4827544
ACGCAGA279050.024.8553337
CGCAGAG286700.024.0937778
ACCTGGG74250.022.9106313
ATAACGC9050.022.8469923
ATGGGGG89000.021.4362135
Produced by FastQC (version 0.11.5)