FastQCFastQC Report
Mon 6 Jun 2016
ERR1211652_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211652_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2381587
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131640.5527406725011516No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107980.453395152056171No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85310.35820652363319083No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78340.32894032424597547No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44290.18596843197414162No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA30270.12710012273328666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG38700.065.622912
GTACCTG41200.062.1375241
TAACGCA4400.061.927384
ACCTGGG42250.058.8215333
TATAACG4500.055.3906022
CCTGGGG32950.043.05854
GTACCCG3750.041.4890981
TATCACG2700.036.57872
ATAACGC7650.033.776033
GTATAAC8000.033.5920221
GGTATCA77950.032.660961
GTATCAA120450.031.4702761
CATGGGG59900.030.744444
ATGGGGG38800.029.543125
CTGGGGG22800.028.4342985
ACAACGC4000.028.1876343
GTACATG128500.028.141261
TACATGG130150.027.2458212
ACATGGG127950.026.2159333
TCAACGC144050.025.7318554
Produced by FastQC (version 0.11.5)