FastQCFastQC Report
Mon 6 Jun 2016
ERR1211652_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211652_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2381587
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149140.6262210870314626No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120160.5045375205692675No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90630.3805445696504054No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85100.35732475865882707No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44530.18697616337341447No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA38850.16312652025729063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG38200.067.451652
TATAACG4500.065.826912
TAACGCA4650.064.673874
GTACCTG42150.061.6192741
ACCTGGG40500.061.379133
CCTGGGG30900.042.8837174
TATCACG2850.039.5951352
GTATAAC9250.038.7288321
ATAACGC8400.036.9219553
TACCGTA2650.035.4653437
GTACCCG4150.032.939161
ACCGTAT3200.032.3067138
TACCCGG4250.029.8710372
CTGGGGG22800.029.6776475
GGTATCA92400.029.2821181
CATGGGG55050.028.5949464
GTATCAA135000.027.3045831
ATGGGGG33750.027.2887785
TGGGGGG37650.026.8350646
ATCACGC4450.026.3997653
Produced by FastQC (version 0.11.5)