##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211652_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2381587 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02129798323555 33.0 31.0 34.0 30.0 34.0 2 32.25954877986821 34.0 31.0 34.0 30.0 34.0 3 32.34498550756281 34.0 31.0 34.0 30.0 34.0 4 35.99689912650682 37.0 35.0 37.0 35.0 37.0 5 35.91167528207032 37.0 35.0 37.0 35.0 37.0 6 35.94947192775238 37.0 35.0 37.0 35.0 37.0 7 35.86806990464761 37.0 35.0 37.0 35.0 37.0 8 35.846985224558246 37.0 35.0 37.0 35.0 37.0 9 37.47591458972526 39.0 37.0 39.0 35.0 39.0 10-11 37.42862217504546 39.0 37.0 39.0 34.0 39.0 12-13 37.33241279869264 39.0 37.0 39.0 34.0 39.0 14-15 38.51500659014346 40.0 38.0 41.0 34.0 41.0 16-17 38.45079730448646 40.0 38.0 41.0 34.0 41.0 18-19 38.474751709679296 40.0 38.0 41.0 34.0 41.0 20-21 38.412328208039426 40.0 38.0 41.0 34.0 41.0 22-23 38.278988968280395 40.0 38.0 41.0 34.0 41.0 24-25 38.16746018516224 40.0 37.0 41.0 33.5 41.0 26-27 37.94787635303686 40.0 37.0 41.0 33.0 41.0 28-29 37.94193556649411 40.0 37.0 41.0 33.0 41.0 30-31 37.77037118526428 40.0 37.0 41.0 33.0 41.0 32-33 37.49956961471489 40.0 36.0 41.0 32.5 41.0 34-35 37.30016350441953 40.0 36.0 41.0 31.5 41.0 36-37 37.16581359404464 40.0 36.0 41.0 31.0 41.0 38-39 37.0753140657889 40.0 36.0 41.0 31.0 41.0 40-41 36.91158101719567 39.0 35.0 41.0 30.5 41.0 42-43 36.72081326443249 39.0 35.0 41.0 30.0 41.0 44-45 36.50165624854351 39.0 35.0 41.0 30.0 41.0 46-47 36.20659753349342 39.0 35.0 41.0 28.5 41.0 48-49 35.91702276675175 39.0 35.0 40.5 27.0 41.0 50-51 35.70382543236926 39.0 35.0 40.0 26.5 41.0 52-53 35.514252051258254 38.5 34.5 40.0 26.0 41.0 54-55 35.304921886120475 38.0 34.0 40.0 26.0 41.0 56-57 35.101460496719206 38.0 34.0 40.0 25.0 41.0 58-59 34.848174557553435 38.0 34.0 40.0 25.0 41.0 60-61 34.525604775303194 37.5 33.0 40.0 23.5 41.0 62-63 34.15829843713457 37.0 33.0 40.0 23.0 41.0 64-65 33.85888506277537 36.5 32.5 39.5 22.5 41.0 66-67 33.50380397608821 36.0 32.0 39.0 22.0 40.5 68-69 33.114502010634084 35.5 31.5 39.0 21.5 40.0 70-71 32.737467705357815 35.0 31.0 38.5 20.5 40.0 72-73 32.32341564679351 35.0 31.0 37.5 20.0 40.0 74-75 31.923437816884288 35.0 30.5 37.0 19.5 39.0 76-77 30.421039206209976 33.5 28.5 35.5 18.5 38.0 78-79 31.29702358133463 34.0 30.0 36.0 19.0 38.5 80-81 31.263593561772048 34.0 30.0 36.0 19.0 37.5 82-83 30.987468650106003 34.0 30.0 35.0 18.5 37.0 84-85 30.652688942289323 34.0 29.5 35.0 18.0 37.0 86-87 30.355417626985705 34.0 29.0 35.0 18.0 36.0 88-89 30.075291601776463 34.0 29.0 35.0 17.0 36.0 90-91 29.78579283477782 34.0 29.0 35.0 15.0 35.5 92-93 29.529421767921978 34.0 29.0 35.0 14.0 35.0 94-95 29.316402046198608 34.0 27.5 35.0 11.5 35.0 96-97 29.088430529726608 33.0 27.0 35.0 11.0 35.0 98-99 28.86512900851407 33.0 27.0 35.0 10.0 35.0 100 27.16476660310961 31.0 24.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.27443723166006606 1101 2 0.16029181690992544 1101 3 0.16821504839119683 1101 4 0.11401778993106149 1101 5 0.09310675342157992 1101 6 0.07536323422446856 1101 7 0.06373462118040152 1101 8 0.17748044992171685 1101 9 0.1172806923078582 1101 10-11 0.11637571089229937 1101 12-13 0.13596477915037752 1101 14-15 0.08882580756717573 1101 16-17 0.04303583737834771 1101 18-19 -0.00222882531294033 1101 20-21 0.006304939087684147 1101 22-23 -0.07148398320930482 1101 24-25 -0.07382814177650943 1101 26-27 -0.08970334563058913 1101 28-29 0.059037456158947066 1101 30-31 0.11021988001543548 1101 32-33 0.048123735189868455 1101 34-35 -0.041379872340250756 1101 36-37 -0.26104005987829026 1101 38-39 -0.2587468326415987 1101 40-41 -0.1943951947448639 1101 42-43 -0.1505970744519729 1101 44-45 -0.02382734383694185 1101 46-47 -0.09306409496186063 1101 48-49 -0.15482273424073867 1101 50-51 -0.25252209008312576 1101 52-53 -0.37771637333968755 1101 54-55 -0.3390607833767376 1101 56-57 -0.44907761006521696 1101 58-59 -0.38765668335839365 1101 60-61 -0.4340284524195468 1101 62-63 -0.5815691038795592 1101 64-65 -0.5593455990940015 1101 66-67 -0.5091988312302504 1101 68-69 -0.5264799562149776 1101 70-71 -0.7116560185152068 1101 72-73 -0.7793716807052604 1101 74-75 -0.885399201882592 1101 76-77 -0.7489372062567412 1101 78-79 -0.6879414063617482 1101 80-81 -0.6938934584265581 1101 82-83 -0.9856300828093296 1101 84-85 -0.7813617646275723 1101 86-87 -0.8943318314423365 1101 88-89 -0.9438059332250894 1101 90-91 -1.1652029376101218 1101 92-93 -1.2131546914330613 1101 94-95 -1.2194775846906296 1101 96-97 -1.3183538243906838 1101 98-99 -1.287103586831158 1101 100 -1.2296946654870347 1102 1 0.311838709638792 1102 2 0.25168514344755977 1102 3 0.18278485888630058 1102 4 0.13200879722597847 1102 5 0.15478259879861866 1102 6 0.1849181953923349 1102 7 0.10365293715984336 1102 8 0.10279672956736619 1102 9 0.08965110627419648 1102 10-11 0.1611589412442882 1102 12-13 0.10338581312489481 1102 14-15 0.19931285871636817 1102 16-17 0.1991968240996087 1102 18-19 0.06998181633743172 1102 20-21 0.116539950256481 1102 22-23 0.15263553055368106 1102 24-25 0.11961237356553767 1102 26-27 0.06332817293573356 1102 28-29 0.09573878538999026 1102 30-31 0.1256685230532142 1102 32-33 0.2142307181898957 1102 34-35 0.2358630036292766 1102 36-37 0.2669647875662804 1102 38-39 0.28646008268416523 1102 40-41 0.14302154810481227 1102 42-43 0.09007982080895971 1102 44-45 -0.010385946524579026 1102 46-47 -0.01372272326055679 1102 48-49 -0.024759927777253665 1102 50-51 0.03268624996392333 1102 52-53 0.027833764128956773 1102 54-55 -0.06415665524680492 1102 56-57 0.06441144756645656 1102 58-59 -0.0817704643142747 1102 60-61 -0.12213482821692168 1102 62-63 -0.1409519112250166 1102 64-65 -0.12121496911277774 1102 66-67 -0.2710996638441543 1102 68-69 -0.34705606940568856 1102 70-71 -0.33882324856868706 1102 72-73 -0.43475738159181176 1102 74-75 -0.5156273777813922 1102 76-77 -0.38219690072186907 1102 78-79 -0.22727813075789172 1102 80-81 -0.16379215056634067 1102 82-83 -0.2807802636980483 1102 84-85 -0.3200199655098821 1102 86-87 -0.32064787533053263 1102 88-89 -0.4735561490534046 1102 90-91 -0.4918375932770367 1102 92-93 -0.6284723958000029 1102 94-95 -0.7222892043103961 1102 96-97 -0.624191365457424 1102 98-99 -0.6586445246709083 1102 100 -0.5907020791260251 1103 1 0.2961667754609181 1103 2 0.15442120195611864 1103 3 0.19413395122011678 1103 4 0.056110925997039374 1103 5 0.10424714104753718 1103 6 0.08380810005473194 1103 7 0.10019858166813123 1103 8 0.14434041508388873 1103 9 0.17702866928843264 1103 10-11 0.16596173607255338 1103 12-13 0.11386315412006098 1103 14-15 0.1028684287829762 1103 16-17 0.17304101895398105 1103 18-19 0.17892787876397165 1103 20-21 0.1745848764677902 1103 22-23 0.22550022952194126 1103 24-25 0.10099215677045237 1103 26-27 0.08236630797556899 1103 28-29 0.20022161156212093 1103 30-31 0.28265885869380014 1103 32-33 0.3904191731983815 1103 34-35 0.28148465701833913 1103 36-37 0.3111988217833357 1103 38-39 0.25911582253005605 1103 40-41 0.2105284045760243 1103 42-43 0.042022487649767015 1103 44-45 0.09472049202837951 1103 46-47 0.18359345801147953 1103 48-49 0.288378377041866 1103 50-51 0.20450941830661407 1103 52-53 0.02522830507188445 1103 54-55 0.15931859615714217 1103 56-57 0.05807737416956371 1103 58-59 0.09655329172594662 1103 60-61 0.04515980414562648 1103 62-63 0.016792636247885184 1103 64-65 -0.11881520419179026 1103 66-67 -0.15512082095528967 1103 68-69 -0.1524694166696321 1103 70-71 -0.15370505606509965 1103 72-73 -0.3697874194656521 1103 74-75 -0.33067569957844256 1103 76-77 -0.051767228526493625 1103 78-79 0.04876013488139108 1103 80-81 0.1273532066231624 1103 82-83 0.12109383843072052 1103 84-85 -0.24050448947488334 1103 86-87 -0.21022603910224902 1103 88-89 -0.1827013756434539 1103 90-91 -0.13404733599611873 1103 92-93 -0.11218011422758423 1103 94-95 -0.17609291333482346 1103 96-97 -0.168418869701906 1103 98-99 -0.27816261269493836 1103 100 -0.28555646073762375 1104 1 0.30869481000366505 1104 2 0.18981179911807544 1104 3 0.192378167894816 1104 4 0.09304909978674658 1104 5 0.19584655974561826 1104 6 0.16003690384437164 1104 7 0.16986642910432437 1104 8 0.1662545193409457 1104 9 0.2703545267437022 1104 10-11 0.2546345581117251 1104 12-13 0.14843088068457888 1104 14-15 0.2001542309027755 1104 16-17 0.2192064873841204 1104 18-19 0.21483445124854939 1104 20-21 0.12235958005271641 1104 22-23 0.17860394160980775 1104 24-25 0.05354216480527185 1104 26-27 0.2328038564465018 1104 28-29 0.21493040165763233 1104 30-31 0.22626331487607843 1104 32-33 0.16491297939847271 1104 34-35 0.23474172940859006 1104 36-37 0.377930116367871 1104 38-39 0.38089036541854426 1104 40-41 0.24350471233066884 1104 42-43 0.2428596811911632 1104 44-45 0.22908857072771838 1104 46-47 0.13081585490375147 1104 48-49 0.09680585753122273 1104 50-51 0.08205313821969895 1104 52-53 0.26265241093590674 1104 54-55 0.1556913979345822 1104 56-57 0.19346377925036506 1104 58-59 0.009989086509605727 1104 60-61 0.08716358400546653 1104 62-63 -0.09905657459283645 1104 64-65 -0.13194288101028206 1104 66-67 -0.07030244287149401 1104 68-69 0.01442991050134168 1104 70-71 0.037263454418599906 1104 72-73 -0.006016607529396367 1104 74-75 0.06596906346416631 1104 76-77 0.08774278705407568 1104 78-79 0.0899258478495959 1104 80-81 0.15138920262671363 1104 82-83 0.08337978028655613 1104 84-85 -0.1923954674175512 1104 86-87 -0.18444054789513942 1104 88-89 -0.2244508359130002 1104 90-91 -0.13602879302379378 1104 92-93 -0.3048914824530051 1104 94-95 -0.19443728581520503 1104 96-97 -0.43713266933439954 1104 98-99 -0.37568439404995857 1104 100 -0.14729393864186235 1105 1 0.2883013916935795 1105 2 0.22899730497024962 1105 3 0.20315692103468308 1105 4 0.14126171536047138 1105 5 0.1484586595799371 1105 6 0.1442650984892353 1105 7 0.15593979503880462 1105 8 0.19359639043281618 1105 9 0.1872429880766049 1105 10-11 0.1936759308336491 1105 12-13 0.22115932251023906 1105 14-15 0.2532319659249467 1105 16-17 0.18235166268207337 1105 18-19 0.1770430117209969 1105 20-21 0.17018088169346868 1105 22-23 0.24136949395868612 1105 24-25 0.17935969860891987 1105 26-27 0.180058646409627 1105 28-29 0.29044062959841455 1105 30-31 0.3356794003338237 1105 32-33 0.32132231080780826 1105 34-35 0.2666427501984998 1105 36-37 0.32671392001278576 1105 38-39 0.21434962735002472 1105 40-41 0.26172126943611573 1105 42-43 0.24713674414878284 1105 44-45 0.2682822602686272 1105 46-47 0.3076284584524629 1105 48-49 0.28488322512247777 1105 50-51 0.32695135985402857 1105 52-53 0.10695973684045867 1105 54-55 0.061729958139807195 1105 56-57 0.23353262244420847 1105 58-59 0.08373130365459502 1105 60-61 0.07881455135087378 1105 62-63 0.1487278169415589 1105 64-65 0.030018665673487988 1105 66-67 -0.08357304572543 1105 68-69 -0.0744153877189504 1105 70-71 -0.022657013487979327 1105 72-73 0.015100983727080575 1105 74-75 -0.14119884578707342 1105 76-77 0.0495677162401158 1105 78-79 0.017068805200885606 1105 80-81 -0.03200091548562867 1105 82-83 -0.012756182516071135 1105 84-85 -0.0048640687750385325 1105 86-87 -0.0307543806673074 1105 88-89 -0.19830294830677886 1105 90-91 -0.16558259827204225 1105 92-93 -0.16762814137022275 1105 94-95 -0.3066227983479415 1105 96-97 -0.19060455752850913 1105 98-99 -0.2485942131403327 1105 100 -0.3741751849675552 1106 1 0.27266591137479423 1106 2 0.18648715143935846 1106 3 0.15019467130799313 1106 4 0.08624433288528621 1106 5 0.0493600660222171 1106 6 0.15219069515771366 1106 7 0.12101656708741615 1106 8 0.11967581375451175 1106 9 0.14469586460227646 1106 10-11 0.14372912464065024 1106 12-13 0.2519986095448843 1106 14-15 0.2373312592708885 1106 16-17 0.1639473151487607 1106 18-19 0.21924890591829893 1106 20-21 0.1929181396063555 1106 22-23 0.09990714124430866 1106 24-25 0.21585010556876938 1106 26-27 0.15248113166126132 1106 28-29 0.17298555138057026 1106 30-31 0.2344617193263403 1106 32-33 0.2711719223593221 1106 34-35 0.27353104287369945 1106 36-37 0.14902663424039986 1106 38-39 0.07123154931175435 1106 40-41 0.16452332707753925 1106 42-43 0.1848928105203882 1106 44-45 0.23046555658800827 1106 46-47 0.2446751640343976 1106 48-49 0.37906737439126914 1106 50-51 0.2319041934681607 1106 52-53 0.10504200354691307 1106 54-55 0.19126768860780174 1106 56-57 0.168204339249165 1106 58-59 0.053159718621088814 1106 60-61 0.17385486839454956 1106 62-63 0.09044156527130554 1106 64-65 0.1750461422635894 1106 66-67 0.028969568736158635 1106 68-69 0.07904214371870211 1106 70-71 0.09555858928054306 1106 72-73 0.01786300159940879 1106 74-75 0.03937539646112498 1106 76-77 0.1479676768935363 1106 78-79 0.14240326765790812 1106 80-81 0.07648639326093232 1106 82-83 0.11982463642098651 1106 84-85 -0.0411474275472159 1106 86-87 -0.0044439117035715014 1106 88-89 0.1099441053207606 1106 90-91 0.1460108128918236 1106 92-93 0.2178555608263224 1106 94-95 0.10763639330424724 1106 96-97 0.15610122245807645 1106 98-99 0.08698402648299464 1106 100 0.1321354523414584 1107 1 0.14449727913946475 1107 2 0.14756115518877522 1107 3 0.11322075308591906 1107 4 0.025189313541666536 1107 5 -0.040752309307286794 1107 6 -0.0162931432290776 1107 7 -0.011902230042430517 1107 8 -0.012136101495720197 1107 9 0.03509976758806488 1107 10-11 0.07290290639605956 1107 12-13 0.08865055679554246 1107 14-15 0.10889094523501086 1107 16-17 0.06338595862194296 1107 18-19 0.01039207922023877 1107 20-21 0.11077921471536456 1107 22-23 0.14061211285059017 1107 24-25 0.10256220802995131 1107 26-27 0.13061005593136343 1107 28-29 0.17495201399738392 1107 30-31 0.09742860486630178 1107 32-33 0.08660229461296609 1107 34-35 0.11063508879514927 1107 36-37 0.10928429228638947 1107 38-39 0.13083559442052461 1107 40-41 0.09624326223148927 1107 42-43 0.05266813072783094 1107 44-45 0.16717661880777257 1107 46-47 0.2664599322344756 1107 48-49 0.13206505757859333 1107 50-51 0.019948747658069976 1107 52-53 0.14974125837647279 1107 54-55 0.09232431382409345 1107 56-57 0.1807273017656854 1107 58-59 0.08646272241569619 1107 60-61 0.10125601376536508 1107 62-63 0.14224371850602324 1107 64-65 0.2879597154310858 1107 66-67 0.19578690787013642 1107 68-69 0.05141365683760313 1107 70-71 0.09402545222776126 1107 72-73 0.21889087389286743 1107 74-75 0.2278484488447532 1107 76-77 0.08344548415565356 1107 78-79 0.23613332631127193 1107 80-81 0.2157392041070061 1107 82-83 0.0031817165153498195 1107 84-85 0.15763709912181056 1107 86-87 0.08679135547105687 1107 88-89 -0.024459717696466043 1107 90-91 -0.03943643727875923 1107 92-93 0.08378128303830579 1107 94-95 0.2412423186493733 1107 96-97 0.1315072335581 1107 98-99 0.2491194665652543 1107 100 0.34423580052976277 1108 1 0.16237453139171976 1108 2 -0.05293824259030089 1108 3 -0.004991771489009977 1108 4 0.026682038280796405 1108 5 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0.19911644595832456 2316 62-63 0.35243987134805366 2316 64-65 0.08324604528975499 2316 66-67 0.17934215121754704 2316 68-69 0.24200192696224576 2316 70-71 0.0692499864051328 2316 72-73 -0.08287781216838397 2316 74-75 0.32549020076308466 2316 76-77 0.269336501981126 2316 78-79 -0.08654253972990134 2316 80-81 0.15352163809248154 2316 82-83 0.23951520864420672 2316 84-85 0.36024201855326865 2316 86-87 0.46627026392678417 2316 88-89 0.48286304362309806 2316 90-91 0.5399532496249186 2316 92-93 0.5053591722420947 2316 94-95 0.618619205019062 2316 96-97 0.6050671061987103 2316 98-99 0.7155988083443532 2316 100 0.31570643546962174 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 7.0 11 16.0 12 40.0 13 144.0 14 440.0 15 1089.0 16 2712.0 17 5778.0 18 10637.0 19 15309.0 20 18744.0 21 23214.0 22 27950.0 23 30203.0 24 30261.0 25 31429.0 26 35011.0 27 40689.0 28 47192.0 29 55475.0 30 66256.0 31 79172.0 32 95577.0 33 119424.0 34 158277.0 35 211481.0 36 297668.0 37 454786.0 38 451166.0 39 71418.0 40 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.65373514831479 19.143308659104992 12.828732454131531 22.37422373844869 2 15.430305841933063 21.70199426320509 41.09565555026083 21.772044344601017 3 16.847823348511138 30.48466795546426 30.91489233053459 21.75261636549001 4 11.070474968392684 17.829884416533805 39.10459869103099 31.99504192404251 5 11.037588491673594 38.09349255536241 34.96909614625584 15.899822806708153 6 27.13046435754565 38.047800400576094 20.996233577010123 13.825501664868135 7 25.844489409792715 33.19870321764437 24.327895642695395 16.62891172986752 8 26.64392813639087 34.50933775955075 21.855778149428506 16.99095595462987 9 27.940486742663612 15.660019978274992 21.70090784002432 34.698585439037075 10-11 24.081512341817064 26.42346796955632 30.24183965421246 19.253180034414157 12-13 24.7746845541769 24.821145892381015 29.99422644916126 20.409943104280824 14-15 22.442896783299705 25.67076610486329 28.364159773327568 23.522177338509433 16-17 21.1433981990985 27.942756609568836 29.19612358996215 21.717721601370517 18-19 20.902067412776923 26.909516758298107 31.524953375982 20.663462452942966 20-21 21.622604970634633 26.66739308525089 31.467655267773427 20.24234667634105 22-23 21.34677871242552 26.402715164870138 31.358109394724902 20.89239672797944 24-25 20.889642074801383 26.590819482975007 31.474474793488543 21.045063648735066 26-27 20.871458368542765 26.432340681038763 31.511652441181443 21.18454850923703 28-29 21.05495757797666 26.548468935724944 31.611692572807748 20.78488091349065 30-31 21.800817620150944 26.58388710526691 31.625402154734783 19.98989311984736 32-33 21.376703043470137 26.687181674976173 31.589431656448003 20.346683625105687 34-35 21.512694034322454 26.901081809451544 31.271360204377714 20.314863951848288 36-37 21.498879722130777 26.91299623102918 31.06323103589592 20.524893010944123 38-39 21.617511670702992 27.384072416620214 30.599675204361958 20.39874070831483 40-41 21.41802700613898 26.9726295827933 31.078601583130077 20.53074182793764 42-43 21.19252062032523 27.190021110610445 30.620568798039027 20.9968894710253 44-45 20.98737074169335 27.459933861739543 30.6310429898693 20.92165240669781 46-47 20.9785781577371 27.008918401570153 30.33585570317262 21.676647737520128 48-49 20.972740293949805 27.2959868179877 30.43511306456171 21.296159823500783 50-51 21.05328244537656 27.642171273611226 30.177526967915647 21.127019313096564 52-53 21.146417461180267 27.717307893781555 29.512529663695624 21.62374498134255 54-55 20.981171056167696 27.64852358237033 29.963173542058584 21.407131819403386 56-57 21.262358308541486 27.764576453026358 29.403735888590994 21.56932934984117 58-59 21.300768389845473 27.704884888487 29.844237244117615 21.15010947754991 60-61 21.83611226124078 28.129852765672254 29.4848268268655 20.549208146221467 62-63 21.488407953691027 27.9847285705617 29.870203768136815 20.656659707610455 64-65 21.349752014892122 27.439738002085335 29.63561663278594 21.574893350236604 66-67 21.1737618344947 27.689568175843814 29.556863104091484 21.57980688557 68-69 21.007856714953714 27.747724215173662 29.770287099654947 21.47413197021768 70-71 21.067763807373872 27.58669775679908 29.863806408077725 21.481732027749327 72-73 20.91529947075949 28.234377641084436 29.04347205293035 21.806850835225728 74-75 21.170401687425645 28.13103248159995 29.066645838398674 21.631919992575728 76-77 20.882609579285415 27.726926875478696 29.687080091640798 21.703383453595087 78-79 20.81055163025619 27.89106190120928 29.492918607822805 21.805467860711726 80-81 20.873747575775326 28.2650372265589 28.912190000006298 21.949025197659473 82-83 21.26465212848518 28.17798745519111 28.985387347011454 21.571973069312254 84-85 20.845724015162602 28.658946207028634 28.570453586628584 21.92487619118018 86-87 20.78169961649524 28.354217796435066 29.008531847021413 21.85555074004828 88-89 20.66202315205904 28.11833507932338 29.16143552089193 22.058206247725646 90-91 21.33502688278743 28.206734001218496 29.02807608455735 21.43016303143672 92-93 20.988524917558873 28.341372520072415 28.956570331526414 21.713532230842294 94-95 20.683404058354757 27.931924209210536 28.841044901813117 22.543626830621587 96-97 20.88846910678315 28.06168249143946 28.966463184803683 22.08338521697371 98-99 20.886614334271755 27.836500008084414 28.905826257372823 22.371059400271008 100 19.874847759438914 28.525765402545044 29.122254420225946 22.477132417790095 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1212.0 1 1079.0 2 3344.5 3 5024.0 4 3044.5 5 1896.0 6 2487.5 7 4331.0 8 6717.5 9 7618.0 10 6253.5 11 4876.0 12 4821.5 13 5200.5 14 5562.0 15 5791.0 16 6036.5 17 6286.0 18 6542.0 19 6774.5 20 7076.0 21 7576.5 22 8389.0 23 9607.5 24 11268.0 25 13295.0 26 16047.5 27 19035.5 28 22230.0 29 26209.5 30 31548.5 31 37096.0 32 43344.0 33 50856.0 34 58522.5 35 64723.5 36 72130.0 37 79172.0 38 82018.5 39 84214.0 40 82140.5 41 78903.5 42 76929.5 43 75393.0 44 76057.0 45 77695.0 46 78137.5 47 76260.0 48 76384.5 49 79082.0 50 84390.5 51 83157.5 52 74633.5 53 69268.5 54 65796.5 55 60800.5 56 55627.5 57 52124.5 58 47507.5 59 40278.5 60 31096.5 61 23449.5 62 18680.0 63 14542.5 64 10725.5 65 7948.0 66 6136.0 67 4672.5 68 3619.0 69 2838.5 70 2125.5 71 1611.0 72 1248.0 73 1003.5 74 791.0 75 563.0 76 408.0 77 283.0 78 188.5 79 133.5 80 96.5 81 61.5 82 48.5 83 40.0 84 19.0 85 8.0 86 7.0 87 6.5 88 6.0 89 5.0 90 2.5 91 1.5 92 1.0 93 1.5 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2560897418402099 2 0.07814117225194797 3 0.0016375635238183614 4 7.557985494546283E-4 5 2.9392165812124434E-4 6 7.138097411515935E-4 7 0.0 8 8.397761660606982E-4 9 0.0 10-11 3.7789927472731415E-4 12-13 0.0015535859072122917 14-15 2.0994404151517455E-5 16-17 8.397761660606982E-5 18-19 0.0014906026947577393 20-21 0.0016795523321213963 22-23 2.0994404151517455E-5 24-25 0.0 26-27 1.8894963736365707E-4 28-29 0.0017215411404244315 30-31 0.0017215411404244315 32-33 0.0032961214517882404 34-35 0.004786724146545979 36-37 0.0014696082906062219 38-39 0.0037579983431216242 40-41 0.006949147774152278 42-43 0.01343641865697117 44-45 0.0089016273602434 46-47 0.006823181349243174 48-49 0.006718209328485585 50-51 0.006130366012243097 52-53 0.004135897617848939 54-55 0.006172354820546131 56-57 0.0038629703638792113 58-59 0.002036457202697193 60-61 0.008943616168546437 62-63 0.011651894304092187 64-65 0.008145828810788772 66-67 0.008439750468910016 68-69 0.01576679751778961 70-71 0.005206612229576329 72-73 0.014465144460395525 74-75 0.00873367212703126 76-77 0.004828712954849015 78-79 0.016018730367607818 80-81 0.006529259691121929 82-83 0.015241937414001674 84-85 0.025088312961063355 86-87 0.01641762404648665 88-89 0.01748833865821404 90-91 0.033108175346943026 92-93 0.027103775759609032 94-95 0.032331382393336876 96-97 0.01920987979863847 98-99 0.019377835031850612 100 0.020448549643578 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2381587.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.30070853205339 #Duplication Level Percentage of deduplicated Percentage of total 1 78.10178415788945 47.09592922338237 2 14.31411371023256 17.263023974708062 3 3.7065286989814497 6.705189202289145 4 1.3990340694119026 3.374509825840788 5 0.69346330527597 2.090816432456032 6 0.40746049608803325 1.4742093967758223 7 0.2580279796181588 1.0891488994448446 8 0.19206210470475557 0.926518479668335 9 0.1326332043231147 0.7198088576004382 >10 0.6947908661247129 7.522412979955129 >50 0.05542537834930217 2.3035820923065886 >100 0.04028323686465485 4.7772282267118165 >500 0.003009876093119398 1.2692572915141616 >1k 0.0010575240327176242 1.2053406827859297 >5k 1.6269600503348065E-4 0.8620157829479548 >10k+ 1.6269600503348065E-4 1.3210086516126116 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14914 0.6262210870314626 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12016 0.5045375205692675 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9063 0.3805445696504054 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8510 0.35732475865882707 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4453 0.18697616337341447 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 3885 0.16312652025729063 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 8.397761660606982E-5 0.0 10-11 0.0 0.0 0.0 8.397761660606982E-5 0.0 12-13 0.0 0.0 0.0 8.397761660606982E-5 0.0 14-15 0.0 0.0 0.0 8.397761660606982E-5 0.0 16-17 0.0 0.0 0.0 8.397761660606982E-5 0.0 18-19 0.0 0.0 0.0 8.397761660606982E-5 4.198880830303491E-5 20-21 0.0 0.0 0.0 1.2596642490910473E-4 4.198880830303491E-5 22-23 0.0 0.0 0.0 1.8894963736365707E-4 4.198880830303491E-5 24-25 0.0 0.0 0.0 4.8287129548490147E-4 4.198880830303491E-5 26-27 0.0 0.0 0.0 9.657425909698029E-4 4.198880830303491E-5 28-29 0.0 0.0 0.0 0.002351373264969955 4.198880830303491E-5 30-31 0.0 0.0 0.0 0.00774693513190994 4.198880830303491E-5 32-33 0.0 0.0 0.0 0.018349109228426256 4.198880830303491E-5 34-35 0.0 0.0 0.0 0.03258331524315509 4.198880830303491E-5 36-37 0.0 0.0 0.0 0.05359871379882406 4.198880830303491E-5 38-39 0.0 0.0 0.0 0.09038090987228264 4.198880830303491E-5 40-41 0.0 0.0 0.0 0.1439796236711067 4.198880830303491E-5 42-43 0.0 0.0 0.0 0.19056620648332392 4.198880830303491E-5 44-45 0.0 0.0 0.0 0.2388743304359656 4.198880830303491E-5 46-47 0.0 0.0 0.0 0.28886200672072865 8.397761660606982E-5 48-49 0.0 0.0 0.0 0.34552590352567425 1.6795523321213964E-4 50-51 0.0 0.0 0.0 0.41050358437462076 1.6795523321213964E-4 52-53 0.0 0.0 0.0 0.48165362004411344 1.6795523321213964E-4 54-55 0.0 0.0 0.0 0.5646444996550619 2.0994404151517454E-4 56-57 0.0 0.0 0.0 0.6434574928398584 2.5193284981820947E-4 58-59 0.0 0.0 0.0 0.7355809382567171 2.5193284981820947E-4 60-61 0.0 0.0 0.0 0.8273054899946968 2.5193284981820947E-4 62-63 0.0 0.0 0.0 0.9326344156228599 2.5193284981820947E-4 64-65 0.0 0.0 0.0 1.027865032854143 2.5193284981820947E-4 66-67 0.0 0.0 0.0 1.1372248840793975 2.5193284981820947E-4 68-69 0.0 0.0 0.0 1.2728907237065032 2.5193284981820947E-4 70-71 0.0 0.0 0.0 1.4188018325595495 2.5193284981820947E-4 72-73 0.0 0.0 0.0 1.540863298296472 2.9392165812124434E-4 74-75 0.0 0.0 0.0 1.667522538542577 2.9392165812124434E-4 76-77 0.0 0.0 0.0 1.8052248353723799 2.9392165812124434E-4 78-79 0.0 0.0 0.0 1.9590298401863968 2.9392165812124434E-4 80-81 0.0 0.0 0.0 2.105633764376443 3.149160622727618E-4 82-83 0.0 0.0 0.0 2.2617271592429753 3.359104664242793E-4 84-85 0.0 0.0 0.0 2.424286830588175 3.359104664242793E-4 86-87 0.0 0.0 0.0 2.587392356441314 3.359104664242793E-4 88 0.0 0.0 0.0 2.7174316957558133 3.359104664242793E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 3820 0.0 67.45165 2 TATAACG 450 0.0 65.82691 2 TAACGCA 465 0.0 64.67387 4 GTACCTG 4215 0.0 61.619274 1 ACCTGGG 4050 0.0 61.37913 3 CCTGGGG 3090 0.0 42.883717 4 TATCACG 285 0.0 39.595135 2 GTATAAC 925 0.0 38.728832 1 ATAACGC 840 0.0 36.921955 3 TACCGTA 265 0.0 35.465343 7 GTACCCG 415 0.0 32.93916 1 ACCGTAT 320 0.0 32.306713 8 TACCCGG 425 0.0 29.871037 2 CTGGGGG 2280 0.0 29.677647 5 GGTATCA 9240 0.0 29.282118 1 CATGGGG 5505 0.0 28.594946 4 GTATCAA 13500 0.0 27.304583 1 ATGGGGG 3375 0.0 27.288778 5 TGGGGGG 3765 0.0 26.835064 6 ATCACGC 445 0.0 26.399765 3 >>END_MODULE