FastQCFastQC Report
Mon 6 Jun 2016
ERR1211651_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211651_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9027774
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239050.26479395695993274No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204190.22617978695523394No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135700.15031390905443578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131950.046.6875651
GTATCAA223950.037.217991
TCAACGC259050.030.9271434
ATCAACG262600.030.5617163
CAACGCA263450.030.4072485
AACGCAG272650.029.4112826
TATCAAC284300.028.3335782
GTACATG280750.027.588041
TACATGG284150.026.47742
ACATGGG282050.025.6387653
ACGCAGA314350.025.4351587
CGCAGAG319650.024.8380328
CATGGGG160100.023.0690784
GCAGAGT365600.021.0094349
GTACCTG72000.020.2438641
GAGTACT233000.019.44926612-13
AGAGTAC330150.018.84139810-11
CAGAGTA352850.018.80766110-11
TACCTGG73700.018.6418882
GAGTACA203300.017.981741
Produced by FastQC (version 0.11.5)