FastQCFastQC Report
Mon 6 Jun 2016
ERR1211651_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211651_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9027774
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277310.30717428238677663No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236010.2614265709354266No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168500.18664623195042324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA141550.044.830381
GTATCAA246950.037.494681
TCAACGC296750.030.4021644
ATCAACG298000.030.212073
CAACGCA301800.029.9247515
AACGCAG312400.029.1058836
TATCAAC317200.028.6734982
GTACATG297200.026.7610721
TACATGG303500.025.4432072
ACGCAGA356250.025.2329797
ACATGGG300100.025.131033
CGCAGAG364950.024.55428
CATGGGG168000.022.7112374
GCAGAGT410750.021.459
GTACCTG72100.021.4474561
TACCTGG74400.020.1007182
GAGTACT258900.019.90998812-13
AGAGTAC376350.019.84607510-11
ATGGGGG96550.019.418565
AGTACTT278450.018.85804712-13
Produced by FastQC (version 0.11.5)