FastQCFastQC Report
Mon 6 Jun 2016
ERR1211650_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211650_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2995797
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148580.495961508740412No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121460.40543468065426325No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91140.3042262209355307No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86920.29013981922006066No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47410.1582550486565011No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG42200.058.5377922
GTACCTG43850.057.5534741
ACCTGGG47600.050.8353423
TATAACG4700.047.0532382
GGTATCA66700.046.818721
TAACGCA4750.044.5134854
ATAACGC5550.040.636263
GTATCAA112650.036.096361
CCTGGGG41050.035.139694
TCAACGC137150.029.2230764
GTATAAC9600.028.9913311
CAACGCA138400.028.957695
ATCAACG137600.028.9562873
AACGCAG143800.028.192296
TATCAAC141950.028.0761492
GTACCCG3900.027.8195821
GTACATG108650.027.526241
TACATGG111100.026.215982
ACGCAGA157250.025.6319897
ACATGGG108950.025.3149053
Produced by FastQC (version 0.11.5)