##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211650_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2995797 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.875899802289673 31.0 31.0 34.0 27.0 34.0 2 30.856005597174974 31.0 31.0 34.0 27.0 34.0 3 30.96749546114106 31.0 31.0 34.0 27.0 34.0 4 34.5152602128916 37.0 35.0 37.0 30.0 37.0 5 34.610578420366934 37.0 35.0 37.0 31.0 37.0 6 34.73783236981678 37.0 35.0 37.0 32.0 37.0 7 34.6992149334551 37.0 35.0 37.0 31.0 37.0 8 34.70332469122574 37.0 35.0 37.0 31.0 37.0 9 36.172748353776974 39.0 37.0 39.0 30.0 39.0 10-11 36.12320661246406 39.0 37.0 39.0 30.0 39.0 12-13 36.08474205695513 39.0 37.0 39.0 30.0 39.0 14-15 37.19285719292729 40.0 37.0 41.0 30.5 41.0 16-17 37.161044456617056 40.0 37.0 41.0 30.5 41.0 18-19 37.21300592129573 40.0 37.0 41.0 30.5 41.0 20-21 37.178033091027196 40.0 37.0 41.0 30.0 41.0 22-23 37.04854350945675 40.0 37.0 41.0 30.0 41.0 24-25 36.943368826392444 40.0 36.5 41.0 30.0 41.0 26-27 36.77876321392938 40.0 36.0 41.0 30.0 41.0 28-29 36.63438794417646 40.0 36.0 41.0 29.0 41.0 30-31 36.397888608607325 39.0 36.0 41.0 28.5 41.0 32-33 36.15453767394787 39.0 35.0 41.0 27.5 41.0 34-35 35.97115225097028 39.0 35.0 41.0 27.0 41.0 36-37 35.77859264162424 39.0 35.0 40.5 26.0 41.0 38-39 35.60359396848318 39.0 35.0 40.0 25.5 41.0 40-41 35.436661429329156 39.0 35.0 40.0 25.0 41.0 42-43 35.4150653398745 39.0 35.0 40.0 25.0 41.0 44-45 35.328746907751096 39.0 35.0 40.0 24.0 41.0 46-47 35.179094411270185 39.0 34.0 40.0 23.5 41.0 48-49 35.116874407711876 39.0 34.0 40.5 23.0 41.0 50-51 34.16420521817733 38.0 33.0 40.0 22.0 40.5 52-53 34.36275705596874 38.0 33.5 39.5 22.0 40.5 54-55 34.86642385982762 38.5 34.0 40.0 22.5 41.0 56-57 34.816039771720185 38.0 34.0 40.0 22.0 41.0 58-59 34.669613795594294 38.0 34.0 40.0 22.0 41.0 60-61 34.45661455031833 38.0 33.5 40.0 22.0 41.0 62-63 34.18740338547639 37.5 33.0 40.0 21.5 41.0 64-65 33.878909352002154 37.0 33.0 40.0 20.5 41.0 66-67 33.649553023786325 36.5 33.0 39.5 20.0 41.0 68-69 33.33115511498276 36.0 32.0 39.0 20.0 41.0 70-71 33.01379399204953 35.5 32.0 39.0 20.0 41.0 72-73 32.68296483373206 35.0 32.0 38.5 19.0 40.0 74-75 32.31659271305766 35.0 31.0 37.5 18.0 40.0 76-77 32.01673544636035 35.0 31.0 37.0 18.0 39.0 78-79 31.7120330583147 35.0 31.0 36.5 18.0 39.0 80-81 31.43529851989304 35.0 31.0 36.0 18.0 38.5 82-83 31.143507387182776 35.0 30.5 36.0 17.5 37.0 84-85 30.840436618369 34.5 30.0 35.0 17.0 37.0 86-87 30.535161594727548 34.0 30.0 35.0 15.0 36.5 88-89 30.31779139240743 34.0 29.5 35.0 15.0 36.0 90-91 30.126192462306356 34.0 29.0 35.0 13.5 36.0 92-93 29.96713528987445 34.0 29.0 35.0 12.5 36.0 94-95 29.804099209659398 34.0 29.0 35.0 11.5 35.0 96-97 29.664538017762887 34.0 29.0 35.0 11.0 35.0 98-99 29.52398994324382 34.0 29.0 35.0 10.0 35.0 100 28.301180954517278 33.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.26045596108998126 1101 2 0.31515201249384006 1101 3 0.27251008316914493 1101 4 0.23581620489190414 1101 5 0.19147681693478802 1101 6 0.1815908849367247 1101 7 0.20567690944402983 1101 8 0.08496671528847344 1101 9 0.16023470125445272 1101 10-11 0.14850850729183662 1101 12-13 0.10760773497700171 1101 14-15 0.2181269976236564 1101 16-17 0.29402225576978935 1101 18-19 0.23808331563220975 1101 20-21 0.13956108935798994 1101 22-23 0.2080709994254235 1101 24-25 0.18064853457658359 1101 26-27 0.15463396811453833 1101 28-29 0.1162115654601763 1101 30-31 0.21714989281224462 1101 32-33 0.10451716207957418 1101 34-35 0.09919499558520783 1101 36-37 0.17891380704500648 1101 38-39 -0.04075798155339072 1101 40-41 -0.04643642541635273 1101 42-43 -0.14795137664383162 1101 44-45 -0.05470495518061824 1101 46-47 -0.25981996760550885 1101 48-49 -0.05180447102863894 1101 50-51 -0.038922654861558215 1101 52-53 0.04706568314656323 1101 54-55 0.013394613646724451 1101 56-57 0.005529260246014189 1101 58-59 -0.05743814513778034 1101 60-61 -0.04003454473211576 1101 62-63 -0.11976966185054749 1101 64-65 -0.04573476003692889 1101 66-67 -0.07227126938717277 1101 68-69 -0.09272729766339438 1101 70-71 -0.15513715923871985 1101 72-73 -0.24861383512683943 1101 74-75 -0.21447346549242496 1101 76-77 -0.2827035846738397 1101 78-79 -0.2336674523159168 1101 80-81 -0.4288059086011238 1101 82-83 -0.3462812808543987 1101 84-85 -0.4347576521702976 1101 86-87 -0.3717337676237129 1101 88-89 -0.4869007125807343 1101 90-91 -0.46231502072103225 1101 92-93 -0.7154371211552792 1101 94-95 -0.6052828035769302 1101 96-97 -0.6677699988549151 1101 98-99 -0.9075574475005297 1101 100 -0.9380160269018738 1102 1 0.34395507492970623 1102 2 0.4080135027990295 1102 3 0.36795618037023914 1102 4 0.25068632951221304 1102 5 0.21004314406467728 1102 6 0.23009892575435487 1102 7 0.23119904449210793 1102 8 0.1591691546971461 1102 9 0.2468848977394913 1102 10-11 0.3110583912618239 1102 12-13 0.21775839420920562 1102 14-15 0.25330112108987635 1102 16-17 0.2507893365009153 1102 18-19 0.27799762841180353 1102 20-21 0.2651758065265284 1102 22-23 0.2511077932184449 1102 24-25 0.21762189136652665 1102 26-27 0.25199599329748423 1102 28-29 0.2483196157790033 1102 30-31 0.29188846300087334 1102 32-33 0.32152172025354986 1102 34-35 0.3200458467370808 1102 36-37 0.14198316202536176 1102 38-39 0.23376603826051223 1102 40-41 0.2437272935933663 1102 42-43 0.2221928562102775 1102 44-45 0.2465875226010894 1102 46-47 0.26465910678684423 1102 48-49 0.2471626123610804 1102 50-51 0.3698302398959825 1102 52-53 0.36617054904140645 1102 54-55 0.4890384990318708 1102 56-57 0.4701707788324967 1102 58-59 0.3616438612432731 1102 60-61 0.22216386059343307 1102 62-63 0.305175788027519 1102 64-65 0.2699248894781263 1102 66-67 0.23361288713926598 1102 68-69 0.2720128611415831 1102 70-71 0.1924687152858695 1102 72-73 0.2024366782969622 1102 74-75 0.2603344895312105 1102 76-77 0.22161319152890968 1102 78-79 0.18057820878928155 1102 80-81 0.08669721099248662 1102 82-83 0.08317516392679991 1102 84-85 0.0817874929723601 1102 86-87 -0.04076358283646542 1102 88-89 -5.686425133824002E-4 1102 90-91 -0.07359138570531343 1102 92-93 0.023705699377490674 1102 94-95 -0.16228629177748033 1102 96-97 -0.20820251872514817 1102 98-99 -0.24249301701295423 1102 100 -0.5308138853860136 1103 1 0.3622363843063283 1103 2 0.4190534288664942 1103 3 0.35190587092046854 1103 4 0.25311045678179056 1103 5 0.27046791717349805 1103 6 0.2325562816314033 1103 7 0.25031263013092797 1103 8 0.22083743596191852 1103 9 0.2524994173474937 1103 10-11 0.3180439811967233 1103 12-13 0.278389229286482 1103 14-15 0.3565023740536901 1103 16-17 0.43048150578930233 1103 18-19 0.37135451614460635 1103 20-21 0.25875030818662736 1103 22-23 0.24728750690827184 1103 24-25 0.21124735099176206 1103 26-27 0.2011383830097344 1103 28-29 0.1729078486593565 1103 30-31 0.25048026583792193 1103 32-33 0.33392435374334895 1103 34-35 0.1424817578967179 1103 36-37 0.11479926197701218 1103 38-39 0.13371447816624027 1103 40-41 0.04496324168358967 1103 42-43 -0.07621286196474131 1103 44-45 -0.022025849839415912 1103 46-47 -0.013224489610017542 1103 48-49 0.11707250262193014 1103 50-51 0.13315212385544584 1103 52-53 0.17093185167418312 1103 54-55 0.19610379302252312 1103 56-57 0.16914546443188527 1103 58-59 0.2545276538896246 1103 60-61 0.19799841156567055 1103 62-63 0.10164722212961408 1103 64-65 0.17900406009208325 1103 66-67 0.18923541283457013 1103 68-69 0.22905730362352728 1103 70-71 0.1957248958068618 1103 72-73 0.20455628433502682 1103 74-75 0.13604277179507562 1103 76-77 0.1345630488912093 1103 78-79 0.02448247650213986 1103 80-81 0.04378904326696542 1103 82-83 0.024832642548982875 1103 84-85 0.009632463900672406 1103 86-87 -0.03511516558863548 1103 88-89 -0.06365627594073686 1103 90-91 -0.023839128390111597 1103 92-93 -0.14424104921441838 1103 94-95 -0.04519024013888284 1103 96-97 -0.045369519015952875 1103 98-99 -0.2670572093303214 1103 100 -0.11991717684684033 1104 1 0.23233573736838053 1104 2 0.24650423407913635 1104 3 0.22877197070622657 1104 4 0.17341472608175934 1104 5 0.2065444741252378 1104 6 0.13791664341214016 1104 7 0.12474504850165147 1104 8 0.2060772479592643 1104 9 0.19102980135851055 1104 10-11 0.22266223875615765 1104 12-13 0.2915320724149737 1104 14-15 0.3455787269212891 1104 16-17 0.30996511599385457 1104 18-19 0.40074077033358435 1104 20-21 0.18315739121995733 1104 22-23 0.2202661761746043 1104 24-25 0.2918833577396782 1104 26-27 0.2661470631547047 1104 28-29 0.23116138772351036 1104 30-31 0.3556926447231845 1104 32-33 0.18218112049007118 1104 34-35 0.14136345566650732 1104 36-37 0.2256027140645429 1104 38-39 0.20398117146019246 1104 40-41 0.09922060754426099 1104 42-43 0.2161949235384455 1104 44-45 0.2644390800975387 1104 46-47 0.17064929442517496 1104 48-49 0.23962535428166376 1104 50-51 0.18360984603123853 1104 52-53 0.26318915797381237 1104 54-55 0.3796242618729835 1104 56-57 0.40444002002773516 1104 58-59 0.44487169331903686 1104 60-61 0.39748758809933094 1104 62-63 0.30836167776802625 1104 64-65 0.45257512078653406 1104 66-67 0.4887320563950581 1104 68-69 0.4202557811383656 1104 70-71 0.35451045505870127 1104 72-73 0.39005639119452695 1104 74-75 0.3868242914073008 1104 76-77 0.2524659792941364 1104 78-79 0.39256200975383493 1104 80-81 0.2904645740171041 1104 82-83 0.320118080051067 1104 84-85 0.25676641754467155 1104 86-87 0.378334329281234 1104 88-89 0.22505237512444154 1104 90-91 0.16228658831307996 1104 92-93 0.14829647861722606 1104 94-95 0.238724732979815 1104 96-97 0.08585406172340626 1104 98-99 0.009140887169067469 1104 100 0.10418800618947444 1105 1 0.3683399350274925 1105 2 0.37815012199452624 1105 3 0.29679555311788164 1105 4 0.28431170690824104 1105 5 0.2705890431391822 1105 6 0.25842599268607813 1105 7 0.17620150837138482 1105 8 0.2502098171120579 1105 9 0.2895160448811538 1105 10-11 0.27962442214848693 1105 12-13 0.31732390645250774 1105 14-15 0.4811060525560862 1105 16-17 0.47142174942552373 1105 18-19 0.36675871748524713 1105 20-21 0.29363824670254957 1105 22-23 0.38447630635030094 1105 24-25 0.3246492070142537 1105 26-27 0.3956280833094894 1105 28-29 0.28825903409655496 1105 30-31 0.3023581176223331 1105 32-33 0.38161903378445317 1105 34-35 0.48604890427544944 1105 36-37 0.4150179542425221 1105 38-39 0.34695208205504713 1105 40-41 0.6159460524449045 1105 42-43 0.5867444883847952 1105 44-45 0.37863754775722924 1105 46-47 0.41377348984914875 1105 48-49 0.38814209814106704 1105 50-51 0.302175089870957 1105 52-53 0.28848559965764053 1105 54-55 0.4447614244907925 1105 56-57 0.4716329392084049 1105 58-59 0.403088983559897 1105 60-61 0.4223183757869293 1105 62-63 0.4221518515374001 1105 64-65 0.4406022040695916 1105 66-67 0.2895899392187289 1105 68-69 0.4375254062318632 1105 70-71 0.3898545458459921 1105 72-73 0.3232613945197258 1105 74-75 0.40619560348056183 1105 76-77 0.31993469263711205 1105 78-79 0.35864705341313297 1105 80-81 0.3132344194496106 1105 82-83 0.34352237905342164 1105 84-85 0.3505810931806792 1105 86-87 0.2872782302510224 1105 88-89 0.22209684804433394 1105 90-91 0.2999755009644325 1105 92-93 0.2730259230623737 1105 94-95 0.09096266608565529 1105 96-97 0.13669046096498505 1105 98-99 0.08247239598537703 1105 100 0.24224213973650066 1106 1 0.2707168333965342 1106 2 0.3218486282470572 1106 3 0.23453653294137666 1106 4 0.16841436696375212 1106 5 0.1679321061011052 1106 6 0.2140913184103468 1106 7 0.10334592474958981 1106 8 0.13544448203237636 1106 9 0.18192256872186618 1106 10-11 0.22677413992688145 1106 12-13 0.17522236904645183 1106 14-15 0.3712420178786999 1106 16-17 0.29338607412069706 1106 18-19 0.2605482830822723 1106 20-21 0.3969123290925438 1106 22-23 0.3813148007526408 1106 24-25 0.2939806068957509 1106 26-27 0.3237632378297093 1106 28-29 0.28016878555443725 1106 30-31 0.3767520428414102 1106 32-33 0.40795358332135834 1106 34-35 0.31443104167527025 1106 36-37 0.4568840471105844 1106 38-39 0.3749378618286485 1106 40-41 0.2569947941419741 1106 42-43 0.2561245364092386 1106 44-45 0.35737020126395436 1106 46-47 0.37569633888398357 1106 48-49 0.3399751352196816 1106 50-51 0.30591538363089654 1106 52-53 0.35990107350925626 1106 54-55 0.477433642035308 1106 56-57 0.45691937074237643 1106 58-59 0.2319657104130428 1106 60-61 0.3395934560875489 1106 62-63 0.4209581009526886 1106 64-65 0.42984223275952615 1106 66-67 0.4998596869344496 1106 68-69 0.40370403223366935 1106 70-71 0.4831716631316709 1106 72-73 0.4421162920779622 1106 74-75 0.5424674717563605 1106 76-77 0.47206304889120787 1106 78-79 0.3662149581126677 1106 80-81 0.22889163715004557 1106 82-83 0.21673159725282076 1106 84-85 0.28298418670361514 1106 86-87 0.3704674902379246 1106 88-89 0.3815402010240305 1106 90-91 0.2666031867473251 1106 92-93 0.3767113317379618 1106 94-95 0.40547758796796884 1106 96-97 0.3553283129623672 1106 98-99 0.1126214588849308 1106 100 0.3369295130486307 1107 1 0.25787707937504933 1107 2 0.3171707779735513 1107 3 0.24213408532334313 1107 4 0.14319964272635133 1107 5 0.027321316911582016 1107 6 0.2061351403040561 1107 7 0.12517241154573355 1107 8 0.13516626066185466 1107 9 0.31090565136192794 1107 10-11 0.3274481474198012 1107 12-13 0.2659418004365932 1107 14-15 0.4540878554578427 1107 16-17 0.391198933747674 1107 18-19 0.3185418243718914 1107 20-21 0.39304947276082913 1107 22-23 0.41390705536981187 1107 24-25 0.3157257768044488 1107 26-27 0.2123855148039553 1107 28-29 0.2578947010031314 1107 30-31 0.36404217277605966 1107 32-33 0.1853423682828037 1107 34-35 0.1837279851945084 1107 36-37 0.14688151229488966 1107 38-39 0.21061701499741758 1107 40-41 0.22857271691120928 1107 42-43 0.23447295189594541 1107 44-45 0.33199578363785776 1107 46-47 0.342335274666965 1107 48-49 0.36291587629840194 1107 50-51 0.2176114795077595 1107 52-53 0.17524243713871357 1107 54-55 0.29157436486334376 1107 56-57 0.40410334259578207 1107 58-59 0.27333993539314605 1107 60-61 0.3400632081318733 1107 62-63 0.38755313372785594 1107 64-65 0.30902206779371966 1107 66-67 0.3022354175950781 1107 68-69 0.2859501831160429 1107 70-71 0.2839713974829934 1107 72-73 0.3254380666517349 1107 74-75 0.3379308107169834 1107 76-77 0.37039691453583856 1107 78-79 0.36058198928582996 1107 80-81 0.34303606080900195 1107 82-83 0.2819103271073722 1107 84-85 0.34356080657758525 1107 86-87 0.3364240536361969 1107 88-89 0.4365300871094142 1107 90-91 0.2720991741340839 1107 92-93 0.22187378807576152 1107 94-95 0.3084653752259534 1107 96-97 0.2999956184025869 1107 98-99 0.09734103222475454 1107 100 0.14396889901487597 1108 1 0.23999141984135264 1108 2 0.40053072687166846 1108 3 0.37116634338785914 1108 4 0.2249626577375281 1108 5 0.25472470241142275 1108 6 0.20900962130053813 1108 7 0.28390763990728374 1108 8 0.2354537087615043 1108 9 0.23584975867588298 1108 10-11 0.32297673253637527 1108 12-13 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-0.26922819803707654 2316 68-69 -0.3626920434523626 2316 70-71 -0.47554434542793445 2316 72-73 -0.3229427662654203 2316 74-75 -0.19394657712874874 2316 76-77 -0.12434504202736463 2316 78-79 -0.1609580364109675 2316 80-81 -0.05860899588389756 2316 82-83 -0.04891228515678492 2316 84-85 -0.13564468659525986 2316 86-87 -0.028916307072055503 2316 88-89 0.07435012699629695 2316 90-91 -0.09774781692178891 2316 92-93 0.10353464327506501 2316 94-95 0.1340393348437594 2316 96-97 0.13602062867501274 2316 98-99 -0.005324283899724946 2316 100 -0.09200048580942521 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 3.0 1 16.0 2 26.0 3 26.0 4 30.0 5 29.0 6 664.0 7 8763.0 8 13465.0 9 10128.0 10 9599.0 11 10047.0 12 11423.0 13 12157.0 14 12576.0 15 12571.0 16 14217.0 17 16709.0 18 20152.0 19 23502.0 20 26335.0 21 30147.0 22 33741.0 23 34933.0 24 33805.0 25 32705.0 26 33832.0 27 37292.0 28 42867.0 29 50270.0 30 59491.0 31 73314.0 32 92088.0 33 121394.0 34 168379.0 35 245693.0 36 369044.0 37 583517.0 38 603147.0 39 147532.0 40 168.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.7574153556205 17.730796439210447 11.630056426239975 23.881731778929073 2 16.423141819888677 20.554243717861322 40.103017613023 22.919596849227002 3 18.105174729163288 27.739602761689735 30.37905453729543 23.776167971851546 4 11.979808381298112 16.665531405660143 38.558104995615885 32.796555217425855 5 12.08346993705434 36.55685841934081 34.79435325288237 16.565318390722474 6 28.24893590103504 36.683616364241466 20.299580035163416 14.767867699560073 7 25.824514951442566 32.51870745404044 24.00522610341182 17.651551491105174 8 26.057411166512946 34.09195680860063 21.70712647407658 18.143505550809845 9 26.941562516690702 15.625066762805107 21.45496181167548 35.97840890882871 10-11 24.314147346178146 25.421108897703576 30.065553768694926 20.199189987423356 12-13 25.088573778155883 24.03009480527956 29.630000535773927 21.251330880790636 14-15 22.47140355073517 25.349334114054635 27.61723713791977 24.56202519729042 16-17 21.508755986835475 27.429538460305768 28.570717939507006 22.490987613351756 18-19 21.367755771704747 26.4433873086844 30.684909463995 21.503947455615847 20-21 22.185602034111973 26.02501842879807 30.495521000817277 21.293858536272683 22-23 21.78457485437817 26.126367526290394 30.356504274745692 21.73255334458574 24-25 21.44715095421077 26.07830039687984 30.666503761996278 21.808044886913112 26-27 21.291305276792926 26.25738637501753 30.495863629990183 21.95544471819936 28-29 21.380250191486706 26.334530445217442 30.47606900332756 21.809150359968292 30-31 21.969782962144404 26.190636757886445 30.549188236354592 21.29039204361456 32-33 21.584163209025174 26.372326848742688 30.593495711022133 21.450014231210005 34-35 21.831541259324005 26.500738174681977 30.325307672943545 21.342412893050476 36-37 21.790106268037988 26.553771066299664 30.07546072409708 21.580661941565264 38-39 21.864161729081417 26.96111870739611 29.652123537297758 21.522596026224715 40-41 22.058706905403298 26.39890743682576 30.124557878383897 21.417827779387054 42-43 21.650801802705796 26.787541608640943 29.84811103406812 21.713545554585146 44-45 21.468356281931378 27.03563089817876 29.712218719583944 21.78379410030592 46-47 21.72817988162836 26.592473796361922 29.43969599540107 22.239650326608647 48-49 21.645103959415877 26.815596199478943 29.448812929811492 22.090486911293684 50-51 21.499984642319625 27.29064810746637 29.2669478680201 21.942419382193904 52-53 21.6586176099894 27.326366572904753 28.88682631231898 22.128189504786867 54-55 21.573912085417238 27.211970566042776 29.20777716197357 22.006340186566415 56-57 21.71025519179068 27.36503781166225 28.720388987673633 22.204318008873436 58-59 21.734519106173565 27.414100378957013 28.830327644332243 22.02105287053718 60-61 21.98350452055474 27.417135684759735 29.09488231431567 21.504477480369857 62-63 21.973914779777193 27.25875795859328 29.13399419541911 21.63333306621042 64-65 21.904675897818887 27.039888947072082 28.824284214569452 22.231150940539575 66-67 21.659236702177378 27.418121279453906 28.74294615173018 22.179695866638536 68-69 21.658031918463312 27.227920291652712 29.041684849330686 22.07236294055329 70-71 21.769209761230986 27.030034094596818 28.96538323368487 22.235372910487328 72-73 21.54352544410311 27.718378522772806 28.41875918258314 22.31933685054094 74-75 21.815932359600087 27.52073691170283 28.622948848951136 22.040381879745947 76-77 21.718892062359675 27.25906432445939 28.883554579570948 22.138489033609986 78-79 21.577500278752762 27.35929996000649 28.718111184629137 22.34508857661161 80-81 21.758895735014864 27.688718047331744 28.20579975482144 22.346586462831947 82-83 21.98726012999622 27.700953937747645 28.41887789783798 21.89290803441815 84-85 21.831420255561838 27.881939396326832 28.166751989707155 22.119888358404175 86-87 21.614260583745327 27.901521665070106 28.30275665026374 22.181461100920828 88-89 21.57830271975576 27.583587679023445 28.518613797486463 22.31949580373433 90-91 22.087628384126106 27.766276456377774 28.3725747068415 21.77352045265462 92-93 21.8269398662091 27.84405818371693 28.32760374603834 22.001398204035628 94-95 21.748873085112706 27.60914765773303 28.299134724335683 22.342844532818578 96-97 22.00744796332807 27.69038854170788 28.151208111466055 22.150955383498 98-99 21.992099010948962 27.4637918637142 28.265877647359723 22.278231477977116 100 22.059982857461495 27.46034994953068 28.217206046701566 22.262461146306258 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1389.0 1 1155.5 2 3674.0 3 5484.5 4 3081.5 5 1892.5 6 2598.5 7 4560.5 8 6992.0 9 7705.0 10 6110.0 11 4456.5 12 4360.5 13 4598.0 14 4805.0 15 5132.0 16 5558.0 17 5926.0 18 6251.5 19 6563.5 20 7053.0 21 7975.0 22 9147.0 23 10911.0 24 13157.0 25 15720.5 26 19178.5 27 23197.0 28 27408.5 29 32271.5 30 38244.0 31 43985.5 32 50568.0 33 58700.5 34 67575.5 35 76318.5 36 85477.0 37 92689.0 38 96126.0 39 97076.0 40 94738.0 41 93314.0 42 93048.5 43 93370.5 44 94826.5 45 97051.0 46 98552.0 47 97930.5 48 99527.0 49 102012.5 50 103570.5 51 101440.0 52 95597.5 53 90921.5 54 87369.0 55 82163.0 56 75328.5 57 69481.0 58 63622.0 59 56594.5 60 46760.0 61 36725.0 62 29685.5 63 23592.5 64 17948.0 65 13807.5 66 10850.0 67 8778.5 68 7136.0 69 5768.0 70 4942.0 71 4299.5 72 3806.0 73 3399.5 74 3066.5 75 2831.0 76 2555.0 77 2181.0 78 1910.0 79 1748.0 80 1581.0 81 1413.0 82 1255.0 83 1098.5 84 913.5 85 713.0 86 554.5 87 444.0 88 326.5 89 239.5 90 182.5 91 131.0 92 83.5 93 51.0 94 38.0 95 23.5 96 16.0 97 13.0 98 11.0 99 14.5 100 38.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24938271852198263 2 0.15548450045179965 3 0.02733830095964446 4 0.029741668076975845 5 0.01719075090868974 6 0.002336606919627732 7 0.0022030865242204326 8 0.003605050675997072 9 0.003905471565663495 10-11 0.00549102626112517 12-13 0.004356102900163129 14-15 0.009229597332529541 16-17 0.015855546954616752 18-19 0.012901408206230262 20-21 0.016189347943134997 22-23 0.02944124718730942 24-25 0.006008417793328453 26-27 0.013919501221210917 28-29 0.027071260168829863 30-31 0.015588506163802154 32-33 0.020678971238705428 34-35 0.01906003644439193 36-37 0.024334092062980237 38-39 0.030442650152864164 40-41 0.03353030929665795 42-43 0.035933676413989334 44-45 0.04631488715690683 46-47 0.010548111237176618 48-49 0.02450099255723936 50-51 0.018459194665059082 52-53 0.02076242148583499 54-55 0.020595520991575866 56-57 0.007560592389938303 58-59 0.011299163461342673 60-61 0.032595666528806855 62-63 0.030743071042530587 64-65 0.01563857631207989 66-67 0.008979246591140855 68-69 0.02430071196412841 70-71 0.020061439409946667 72-73 0.033279958555269266 74-75 0.037953172394524726 76-77 0.010130860001528809 78-79 0.010381210742917495 80-81 0.014320062407432815 82-83 0.02112960257320506 84-85 0.01063156148430618 86-87 0.009863819210714211 88-89 0.02314909855374046 90-91 0.016322868338542296 92-93 0.017557931996059813 94-95 0.015054424582172958 96-97 0.016072517597153613 98-99 0.028556674567736064 100 0.030309129757456862 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2995797.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.89524986939027 #Duplication Level Percentage of deduplicated Percentage of total 1 77.827962088841 49.7283708449193 2 14.539659587617853 18.58030364733437 3 3.8949266955019395 7.46601943296165 4 1.3954837288038024 3.5665912616234965 5 0.6756261048393756 2.158464939349739 6 0.39888000762355247 1.5291922653006709 7 0.25704772130760895 1.149688986691495 8 0.17961774801821154 0.9181376712480643 9 0.13036511675660659 0.7496740538474053 >10 0.637318587549467 7.128882144043956 >50 0.04113182405520579 1.7893988212991014 >100 0.020168363428321835 2.4144962228168474 >500 0.001187451292568247 0.5086252604606772 >1k 3.7498461869839555E-4 0.5227615046392787 >5k 1.2499487289946518E-4 0.7110450959901456 >10k+ 1.2499487289946518E-4 1.078347847473767 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14858 0.495961508740412 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12146 0.40543468065426325 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9114 0.3042262209355307 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8692 0.29013981922006066 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4741 0.1582550486565011 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.338009885182474E-5 2 0.0 0.0 0.0 0.0 3.338009885182474E-5 3 0.0 0.0 0.0 0.0 3.338009885182474E-5 4 0.0 0.0 0.0 0.0 3.338009885182474E-5 5 0.0 0.0 0.0 0.0 3.338009885182474E-5 6 3.338009885182474E-5 0.0 0.0 0.0 3.338009885182474E-5 7 3.338009885182474E-5 0.0 0.0 0.0 6.676019770364948E-5 8 3.338009885182474E-5 0.0 0.0 0.0 1.0014029655547421E-4 9 3.338009885182474E-5 0.0 0.0 3.338009885182474E-5 1.669004942591237E-4 10-11 3.338009885182474E-5 0.0 0.0 3.338009885182474E-5 1.669004942591237E-4 12-13 3.338009885182474E-5 3.338009885182474E-5 0.0 3.338009885182474E-5 1.669004942591237E-4 14-15 3.338009885182474E-5 5.007014827773711E-5 0.0 3.338009885182474E-5 1.669004942591237E-4 16-17 3.338009885182474E-5 6.676019770364948E-5 0.0 3.338009885182474E-5 1.669004942591237E-4 18-19 3.338009885182474E-5 8.345024712956185E-5 0.0 3.338009885182474E-5 1.669004942591237E-4 20-21 3.338009885182474E-5 1.0014029655547421E-4 0.0 1.5021044483321132E-4 1.669004942591237E-4 22-23 3.338009885182474E-5 1.0014029655547421E-4 0.0 2.5035074138868556E-4 1.669004942591237E-4 24-25 3.338009885182474E-5 1.0014029655547421E-4 0.0 5.173915322032835E-4 1.669004942591237E-4 26-27 3.338009885182474E-5 1.0014029655547421E-4 0.0 9.8471291612883E-4 1.669004942591237E-4 28-29 3.338009885182474E-5 1.0014029655547421E-4 0.0 0.0019527357828317473 1.669004942591237E-4 30-31 3.338009885182474E-5 1.0014029655547421E-4 0.0 0.005858207348495242 1.669004942591237E-4 32-33 3.338009885182474E-5 1.0014029655547421E-4 0.0 0.01393619127063683 1.669004942591237E-4 34-35 3.338009885182474E-5 1.0014029655547421E-4 0.0 0.025018384089442642 1.669004942591237E-4 36-37 3.338009885182474E-5 1.0014029655547421E-4 0.0 0.04376130959474223 1.669004942591237E-4 38-39 3.338009885182474E-5 1.0014029655547421E-4 0.0 0.07430410004416188 1.669004942591237E-4 40-41 3.338009885182474E-5 1.0014029655547421E-4 0.0 0.11314184505825996 1.8359054368503606E-4 42-43 3.338009885182474E-5 1.0014029655547421E-4 0.0 0.14932587221363797 2.0028059311094843E-4 44-45 3.338009885182474E-5 1.0014029655547421E-4 0.0 0.18657806253227438 2.0028059311094843E-4 46-47 3.338009885182474E-5 1.0014029655547421E-4 0.0 0.22825311594877756 2.169706425368608E-4 48-49 3.338009885182474E-5 1.0014029655547421E-4 0.0 0.2752689851815727 2.3366069196277317E-4 50-51 3.338009885182474E-5 1.0014029655547421E-4 0.0 0.32543927375586534 2.3366069196277317E-4 52-53 3.338009885182474E-5 1.0014029655547421E-4 0.0 0.3772785672727491 2.8373084024051025E-4 54-55 3.338009885182474E-5 1.0014029655547421E-4 0.0 0.43844759841871794 3.338009885182474E-4 56-57 3.338009885182474E-5 1.0014029655547421E-4 0.0 0.5026208384613511 3.338009885182474E-4 58-59 3.338009885182474E-5 1.0014029655547421E-4 0.0 0.5760069857870878 3.338009885182474E-4 60-61 3.338009885182474E-5 1.0014029655547421E-4 0.0 0.6500941151887127 3.338009885182474E-4 62-63 3.338009885182474E-5 1.0014029655547421E-4 0.0 0.7296555808020371 3.671810873700721E-4 64-65 3.338009885182474E-5 1.0014029655547421E-4 0.0 0.8047441131692168 3.671810873700721E-4 66-67 3.338009885182474E-5 1.0014029655547421E-4 0.0 0.889462804055148 4.0056118622189686E-4 68-69 3.338009885182474E-5 1.0014029655547421E-4 0.0 0.9893861299680853 4.339412850737216E-4 70-71 3.338009885182474E-5 1.0014029655547421E-4 0.0 1.0991565850423108 4.339412850737216E-4 72-73 3.338009885182474E-5 1.0014029655547421E-4 0.0 1.1963594328988245 4.6732138392554634E-4 74-75 3.338009885182474E-5 1.669004942591237E-4 0.0 1.2932785499150978 4.8401143335145873E-4 76-77 3.338009885182474E-5 1.669004942591237E-4 0.0 1.4000781761915109 5.007014827773711E-4 78-79 3.338009885182474E-5 1.669004942591237E-4 0.0 1.5251033364410205 5.007014827773711E-4 80-81 3.338009885182474E-5 1.669004942591237E-4 0.0 1.6420171326695367 5.173915322032835E-4 82-83 3.338009885182474E-5 1.669004942591237E-4 0.0 1.7698629112720254 5.340815816291958E-4 84-85 3.338009885182474E-5 1.669004942591237E-4 0.0 1.9004625480297896 5.340815816291958E-4 86-87 3.338009885182474E-5 1.669004942591237E-4 0.0 2.0355017379348466 5.340815816291958E-4 88 3.338009885182474E-5 1.669004942591237E-4 0.0 2.1450385323171095 5.340815816291958E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 4220 0.0 58.537792 2 GTACCTG 4385 0.0 57.553474 1 ACCTGGG 4760 0.0 50.835342 3 TATAACG 470 0.0 47.053238 2 GGTATCA 6670 0.0 46.81872 1 TAACGCA 475 0.0 44.513485 4 ATAACGC 555 0.0 40.63626 3 GTATCAA 11265 0.0 36.09636 1 CCTGGGG 4105 0.0 35.13969 4 TCAACGC 13715 0.0 29.223076 4 GTATAAC 960 0.0 28.991331 1 CAACGCA 13840 0.0 28.95769 5 ATCAACG 13760 0.0 28.956287 3 AACGCAG 14380 0.0 28.19229 6 TATCAAC 14195 0.0 28.076149 2 GTACCCG 390 0.0 27.819582 1 GTACATG 10865 0.0 27.52624 1 TACATGG 11110 0.0 26.21598 2 ACGCAGA 15725 0.0 25.631989 7 ACATGGG 10895 0.0 25.314905 3 >>END_MODULE