FastQCFastQC Report
Mon 6 Jun 2016
ERR1211650_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211650_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2995797
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170000.5674616804810206No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142170.4745648653763923No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107860.36003774621578166No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96430.32188429322814593No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51320.17130666730756455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG43600.060.62852
GTACCTG48100.055.108311
ACCTGGG49950.052.2112243
TAACGCA4550.050.6038784
TATAACG4600.048.0581472
GGTATCA81700.044.0482181
TACCGTA2950.041.41577
CCTGGGG41200.038.5494884
GTATCAA130850.034.171221
ATAACGC6850.033.6133583
ACCGTAT3650.032.1855358
TATCACG3850.031.7643262
GTATAAC9750.028.0575161
ATCAACG160200.027.161573
TCAACGC160600.027.093474
CAACGCA162400.026.793625
CTGGGGG27900.026.7793065
TATCAAC164550.026.612082
AACGCAG168550.026.1788446
GTACCGT4500.026.1060496
Produced by FastQC (version 0.11.5)