##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1211650_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2995797
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.988443809777497	33.0	31.0	34.0	30.0	34.0
2	32.23788360826852	34.0	31.0	34.0	30.0	34.0
3	32.32368481576022	34.0	31.0	34.0	30.0	34.0
4	35.980179898704755	37.0	35.0	37.0	35.0	37.0
5	35.88947381948777	37.0	35.0	37.0	35.0	37.0
6	35.926397549633705	37.0	35.0	37.0	35.0	37.0
7	35.83874408045672	37.0	35.0	37.0	35.0	37.0
8	35.81777203195009	37.0	35.0	37.0	35.0	37.0
9	37.44643345326803	39.0	37.0	39.0	34.0	39.0
10-11	37.40215575354405	39.0	37.0	39.0	34.0	39.0
12-13	37.3054043715245	39.0	37.0	39.0	34.0	39.0
14-15	38.486403618135675	40.0	38.0	41.0	34.0	41.0
16-17	38.4290664554374	40.0	38.0	41.0	34.0	41.0
18-19	38.44600034648543	40.0	38.0	41.0	34.0	41.0
20-21	38.37710031754488	40.0	38.0	41.0	34.0	41.0
22-23	38.23757150434425	40.0	38.0	41.0	34.0	41.0
24-25	38.12087734916618	40.0	37.0	41.0	33.0	41.0
26-27	37.90076614002885	40.0	37.0	41.0	33.0	41.0
28-29	37.892330822148494	40.0	37.0	41.0	33.0	41.0
30-31	37.7269588026158	40.0	37.0	41.0	33.0	41.0
32-33	37.47910389121826	40.0	36.0	41.0	32.0	41.0
34-35	37.27505368354397	39.5	36.0	41.0	31.5	41.0
36-37	37.12924223503796	39.0	36.0	41.0	31.0	41.0
38-39	37.02320584472179	39.0	36.0	41.0	31.0	41.0
40-41	36.848368564358665	39.0	35.0	41.0	30.0	41.0
42-43	36.645277033123406	39.0	35.0	41.0	30.0	41.0
44-45	36.42118758380491	39.0	35.0	41.0	29.5	41.0
46-47	36.12338903470429	39.0	35.0	40.5	28.5	41.0
48-49	35.8344797728284	39.0	35.0	40.0	27.0	41.0
50-51	35.61367826324681	39.0	35.0	40.0	26.0	41.0
52-53	35.40818520079965	38.0	34.0	40.0	26.0	41.0
54-55	35.18866648841694	38.0	34.0	40.0	25.0	41.0
56-57	34.96791788629203	38.0	34.0	40.0	25.0	41.0
58-59	34.69835389380522	38.0	33.0	40.0	24.0	41.0
60-61	34.37097623770903	37.0	33.0	40.0	23.5	41.0
62-63	34.01593465778889	37.0	32.5	40.0	23.0	41.0
64-65	33.698869115631	36.0	32.0	39.0	22.0	41.0
66-67	33.33626377221154	36.0	31.5	39.0	22.0	40.0
68-69	32.94257337863681	35.0	31.0	39.0	21.0	40.0
70-71	32.568067696175675	35.0	31.0	38.0	20.0	40.0
72-73	32.14951113176227	35.0	30.5	37.5	20.0	39.5
74-75	31.7550833718039	35.0	30.0	37.0	19.0	39.0
76-77	30.29478649588073	33.0	28.5	35.5	18.5	37.5
78-79	31.16069563458405	34.0	30.0	36.0	19.0	38.0
80-81	31.125025494050497	34.0	30.0	36.0	19.0	37.5
82-83	30.84328744571144	34.0	29.5	35.0	18.0	37.0
84-85	30.514210742583693	34.0	29.0	35.0	18.0	36.5
86-87	30.21660162554405	34.0	29.0	35.0	17.5	36.0
88-89	29.934464184322238	34.0	29.0	35.0	16.5	36.0
90-91	29.639562026398984	34.0	29.0	35.0	15.0	35.5
92-93	29.39183713048648	34.0	27.5	35.0	12.5	35.0
94-95	29.175627387302946	33.0	27.0	35.0	11.5	35.0
96-97	28.918545548980788	33.0	27.0	35.0	10.5	35.0
98-99	28.70450684742658	33.0	26.0	35.0	10.0	35.0
100	26.971912315821132	31.0	24.0	34.0	8.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.21550536911497176
1101	2	0.1584333752065774
1101	3	0.11900577577921467
1101	4	0.10316049707523689
1101	5	0.014065260430065507
1101	6	0.08939790744820897
1101	7	0.08348051955407954
1101	8	0.07088596280981108
1101	9	0.06601468338125471
1101	10-11	0.10236726722628475
1101	12-13	0.06627127758758178
1101	14-15	0.07502648993241934
1101	16-17	0.09216404316405402
1101	18-19	0.12324397196277914
1101	20-21	0.07446119009691898
1101	22-23	0.012877236273226345
1101	24-25	-0.03186255232029822
1101	26-27	-0.11431968724858166
1101	28-29	0.1020945205217032
1101	30-31	0.10208498399072141
1101	32-33	0.09297232266946054
1101	34-35	0.06020770581707069
1101	36-37	-0.07135133516729297
1101	38-39	-0.10604890270720801
1101	40-41	-0.14666405041446495
1101	42-43	-0.06846772401819834
1101	44-45	-0.04953347737063751
1101	46-47	-0.20439569398217117
1101	48-49	-0.2114523132236883
1101	50-51	-0.4519302453569054
1101	52-53	-0.33125566115672456
1101	54-55	-0.4267303363855106
1101	56-57	-0.45074001124907426
1101	58-59	-0.5377748537886902
1101	60-61	-0.5142286191232728
1101	62-63	-0.6880637784232988
1101	64-65	-0.6957473031373524
1101	66-67	-0.619860556385639
1101	68-69	-0.5909666566082663
1101	70-71	-0.7544816072514422
1101	72-73	-0.9465214501957249
1101	74-75	-1.0153429148758022
1101	76-77	-0.95032894438231
1101	78-79	-0.6501846610512061
1101	80-81	-0.7221422220557017
1101	82-83	-0.8440011637397582
1101	84-85	-0.8561955695825283
1101	86-87	-0.8914190803381068
1101	88-89	-1.0263030468290566
1101	90-91	-1.1381472793956249
1101	92-93	-1.2149761887412716
1101	94-95	-1.4210634414914587
1101	96-97	-1.4362178924859776
1101	98-99	-1.3240478053549793
1101	100	-1.3446772890388168
1102	1	0.20029551158705772
1102	2	0.17373410420497493
1102	3	0.17152185861302627
1102	4	0.09118779948481404
1102	5	0.08705183084706647
1102	6	0.11499860164522602
1102	7	0.01973440093172485
1102	8	0.1116867082609545
1102	9	0.09217858456975137
1102	10-11	0.16589775678379937
1102	12-13	0.1873003087342653
1102	14-15	0.21211438400025173
1102	16-17	0.17403602378511351
1102	18-19	0.0648094215401187
1102	20-21	0.06717845190633653
1102	22-23	0.02862306114319324
1102	24-25	0.07775192165265565
1102	26-27	0.06169998544415023
1102	28-29	0.1312301585037119
1102	30-31	0.12225240323441966
1102	32-33	0.2117240101657316
1102	34-35	0.13489435723389676
1102	36-37	0.14833262388258106
1102	38-39	0.33596071659363247
1102	40-41	0.0904957236402737
1102	42-43	0.11661419868264744
1102	44-45	0.16723687429759337
1102	46-47	0.09178504566801138
1102	48-49	0.0474053125379541
1102	50-51	0.09594229443968061
1102	52-53	0.04703622323646073
1102	54-55	0.03643141548568707
1102	56-57	0.15920444383699106
1102	58-59	0.06635785865496757
1102	60-61	0.19395132774415913
1102	62-63	0.18420465677215958
1102	64-65	0.10770108393763422
1102	66-67	-0.051477868076311495
1102	68-69	-0.10984406053457008
1102	70-71	-0.29734602854291126
1102	72-73	-0.4384180903266106
1102	74-75	-0.39107922983937016
1102	76-77	-0.10319458818924332
1102	78-79	-0.1831825544456791
1102	80-81	-0.21286091078691882
1102	82-83	-0.26052939850805856
1102	84-85	-0.25846025224388214
1102	86-87	-0.24228581329518661
1102	88-89	-0.36926140132759855
1102	90-91	-0.4741652980968425
1102	92-93	-0.571250536901907
1102	94-95	-0.5362489140852098
1102	96-97	-0.5002344463478074
1102	98-99	-0.48297176511090356
1102	100	-0.6899021458072916
1103	1	0.2808911644823624
1103	2	0.22057729729127828
1103	3	0.19870196747797308
1103	4	0.14908630638298348
1103	5	0.13261717211601365
1103	6	0.16795942667809527
1103	7	0.20663907363216794
1103	8	0.18066729026558903
1103	9	0.24574269838732477
1103	10-11	0.16164137287697855
1103	12-13	0.25652419550900873
1103	14-15	0.248355867218244
1103	16-17	0.2044972903793436
1103	18-19	0.18816839665389296
1103	20-21	0.22705209845386776
1103	22-23	0.2199916292890478
1103	24-25	0.05673928764546332
1103	26-27	0.10224145117809513
1103	28-29	0.24655349738555543
1103	30-31	0.36978913757148035
1103	32-33	0.3993754344910414
1103	34-35	0.39904149261571575
1103	36-37	0.3403916334292205
1103	38-39	0.46529972599755354
1103	40-41	0.3153077701478324
1103	42-43	0.22928170208579246
1103	44-45	0.16089545102490632
1103	46-47	0.34463389698404256
1103	48-49	0.41506703104125364
1103	50-51	0.30887723238024023
1103	52-53	0.3005432908943604
1103	54-55	0.21111453945649572
1103	56-57	0.21099925696287158
1103	58-59	0.0134564659971943
1103	60-61	-0.019969429386385684
1103	62-63	-0.04865986328186267
1103	64-65	0.16536464897025382
1103	66-67	0.1112486439814333
1103	68-69	0.11931190161426031
1103	70-71	0.06248845731017383
1103	72-73	-0.14241341780457617
1103	74-75	-0.13774053532668162
1103	76-77	-0.1402101418536681
1103	78-79	-0.004180269031120787
1103	80-81	0.00248979151865214
1103	82-83	0.07254557682695051
1103	84-85	-0.22747870148038274
1103	86-87	-0.14123204271797718
1103	88-89	-0.10962410161358349
1103	90-91	-0.11061322240162852
1103	92-93	-0.30436839721151543
1103	94-95	-0.19969987668897815
1103	96-97	-0.19776982031905987
1103	98-99	-0.24677301075995928
1103	100	-0.3430487834290332
1104	1	0.21719457890628746
1104	2	0.1532678531884386
1104	3	0.13419079715618665
1104	4	0.14391807273555202
1104	5	0.1283000919381081
1104	6	0.09264077513993385
1104	7	0.12283122278701342
1104	8	0.14857987743929613
1104	9	0.14922075627714548
1104	10-11	0.15360326082164732
1104	12-13	0.1418652612661475
1104	14-15	0.19609631424133056
1104	16-17	0.21314918766108093
1104	18-19	0.24754976333440482
1104	20-21	0.09409840102743061
1104	22-23	0.07658715993407839
1104	24-25	-0.05607183117354708
1104	26-27	0.07123084493434106
1104	28-29	0.0686622032819244
1104	30-31	0.25815763381300627
1104	32-33	0.27548946672056474
1104	34-35	0.24441732274764405
1104	36-37	0.21671456723063898
1104	38-39	0.1527392890647974
1104	40-41	0.19154979291065644
1104	42-43	0.08933976533611343
1104	44-45	0.1425466011660106
1104	46-47	0.19268570369797544
1104	48-49	0.09980269829126343
1104	50-51	-0.02481543793366825
1104	52-53	0.025769403853615813
1104	54-55	-0.027879916987750164
1104	56-57	0.0812730231297607
1104	58-59	-0.10770283261905433
1104	60-61	-0.07029729974654231
1104	62-63	-0.0726228881415878
1104	64-65	-0.13176269164704024
1104	66-67	-0.076104095738593
1104	68-69	-0.35167081605067096
1104	70-71	-0.12824513756617506
1104	72-73	-0.08906271542230115
1104	74-75	-0.25148393762819055
1104	76-77	0.030485434688394264
1104	78-79	-0.004115890559283741
1104	80-81	0.019223486247330612
1104	82-83	0.05576436465477741
1104	84-85	-0.26863231114188224
1104	86-87	-0.2155401274277864
1104	88-89	-0.1566073570796256
1104	90-91	-0.10057094720512083
1104	92-93	-0.206019332757716
1104	94-95	-0.22403395365935452
1104	96-97	-0.3727973672978244
1104	98-99	-0.2739832069084649
1104	100	-0.13276614004022136
1105	1	0.2789544800860426
1105	2	0.17164573479294631
1105	3	0.13129337099515226
1105	4	0.13110242811590922
1105	5	0.13042559076994564
1105	6	0.03784684608274347
1105	7	0.08793363068382831
1105	8	0.06983503508645583
1105	9	0.10218779771061293
1105	10-11	0.10613639739499803
1105	12-13	0.1642554928612796
1105	14-15	0.2038760167222975
1105	16-17	0.16320864320996264
1105	18-19	0.16254060476694576
1105	20-21	0.12560677285129884
1105	22-23	0.13940525982025065
1105	24-25	0.1279623537562813
1105	26-27	0.07372061218777759
1105	28-29	0.09342631778370247
1105	30-31	0.24169676504657645
1105	32-33	0.2974935229902016
1105	34-35	0.27079238841544395
1105	36-37	0.16206858914769384
1105	38-39	0.09142080670737585
1105	40-41	0.06737955658278594
1105	42-43	0.018346157072187452
1105	44-45	0.011853610655613522
1105	46-47	0.005171390129106612
1105	48-49	0.015444185353068463
1105	50-51	-0.025104990054948928
1105	52-53	-0.008866998689867955
1105	54-55	-0.14581007227489096
1105	56-57	-0.1360635714748426
1105	58-59	-0.149311874603697
1105	60-61	-0.06391519423696224
1105	62-63	-0.048663190918283306
1105	64-65	0.026014216909942434
1105	66-67	-0.11373285436007308
1105	68-69	-0.06619750627942267
1105	70-71	-0.03284998993481025
1105	72-73	-0.0532880005189611
1105	74-75	-0.1619504213351739
1105	76-77	-0.16953793267577666
1105	78-79	-0.08747367072741596
1105	80-81	-0.07813650958718554
1105	82-83	-0.23834650925500966
1105	84-85	-0.21883749522548968
1105	86-87	-0.19251491311053215
1105	88-89	-0.32326068910912653
1105	90-91	-0.11937056316757122
1105	92-93	-0.25264960644797085
1105	94-95	-0.2503481668162806
1105	96-97	-0.21011934460015524
1105	98-99	-0.19986664778670615
1105	100	-0.1776672499476888
1106	1	0.21095310795893596
1106	2	0.1189261164937605
1106	3	0.11977436391622831
1106	4	0.10112269817547315
1106	5	0.060145240253838494
1106	6	0.12536360786276646
1106	7	0.09877031637316946
1106	8	0.11463747996749163
1106	9	0.1337585714031988
1106	10-11	0.05809510884290603
1106	12-13	0.06280758691435295
1106	14-15	0.09620333140717463
1106	16-17	0.06309388348812917
1106	18-19	0.057919654880763005
1106	20-21	0.10609294243374023
1106	22-23	0.05236967806953885
1106	24-25	0.0921641811801166
1106	26-27	0.13693076592838338
1106	28-29	0.0856204737696018
1106	30-31	0.14542115460593408
1106	32-33	0.048733738788371284
1106	34-35	0.04703026536058985
1106	36-37	0.08361753354536461
1106	38-39	0.017460972610010117
1106	40-41	0.10964574924191339
1106	42-43	0.11208270866728043
1106	44-45	0.08413102980926368
1106	46-47	0.00745910023607621
1106	48-49	0.022083097630492432
1106	50-51	0.015863295098007768
1106	52-53	-0.010614277824181784
1106	54-55	-0.04958716398910923
1106	56-57	0.04277916404765847
1106	58-59	0.1428379990982407
1106	60-61	0.05310354777195414
1106	62-63	-0.009842596537765758
1106	64-65	0.11570049875732735
1106	66-67	0.09570083136045326
1106	68-69	0.09446579243888209
1106	70-71	0.07817661062028236
1106	72-73	-0.05958918241936573
1106	74-75	0.009020982288493684
1106	76-77	0.18830127897482996
1106	78-79	0.13271094273813944
1106	80-81	0.15416710472035788
1106	82-83	0.23843117496864608
1106	84-85	0.22108111268918762
1106	86-87	-0.04276127229598714
1106	88-89	0.09308882908715077
1106	90-91	0.10354957279775334
1106	92-93	0.21638460317563002
1106	94-95	0.23993717325295094
1106	96-97	0.12217210767165199
1106	98-99	0.09836829779598233
1106	100	0.09821137917259293
1107	1	0.26860191431422464
1107	2	0.17435006441850476
1107	3	0.1170110092397465
1107	4	0.1498278636228889
1107	5	0.11186622802082269
1107	6	0.1614335282188577
1107	7	0.13000431534910462
1107	8	0.10398557606742287
1107	9	0.17681282152867084
1107	10-11	0.1705679736409067
1107	12-13	0.18321346160320928
1107	14-15	0.2104001243583582
1107	16-17	0.20737772201022864
1107	18-19	0.1925103581907308
1107	20-21	0.10772916596392434
1107	22-23	0.17067195991062079
1107	24-25	0.21274009130569027
1107	26-27	0.19508633263713193
1107	28-29	0.2349884935208877
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2316	82-83	-0.19639308984310944
2316	84-85	-0.08944914633341128
2316	86-87	0.16652620455482747
2316	88-89	0.10162540355428362
2316	90-91	0.22948961579441018
2316	92-93	0.17488160681495302
2316	94-95	0.21149115987731548
2316	96-97	0.30849897164630846
2316	98-99	0.24841472028531797
2316	100	0.03388371087244835
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	5.0
11	20.0
12	58.0
13	141.0
14	480.0
15	1292.0
16	3170.0
17	6919.0
18	12680.0
19	18772.0
20	23751.0
21	29507.0
22	35111.0
23	38596.0
24	39576.0
25	40759.0
26	45294.0
27	52902.0
28	61566.0
29	73025.0
30	86593.0
31	103946.0
32	124740.0
33	154948.0
34	203249.0
35	276200.0
36	385898.0
37	569964.0
38	528200.0
39	78421.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.336612707575185	19.12765298866441	12.61836282260426	22.917371481156145
2	15.787068493809642	21.602718666883135	40.04334687589151	22.566865963415715
3	17.03454362649996	29.150282333494005	31.242581151685656	22.57259288832038
4	11.375666232609188	17.74572955203733	38.82770432774556	32.05089988760793
5	11.453906982799195	37.25932057988043	35.221527543652925	16.065244893667447
6	28.03618554354089	37.585044572669176	20.291863693686658	14.086906190103285
7	25.55009568405336	33.067060284792326	24.242430311533127	17.14041371962119
8	26.22905509989562	34.64894082570231	21.62524113109888	17.49676294330319
9	26.94344777032623	15.690949687178405	21.829950427215195	35.53565211528017
10-11	24.242553211375437	25.975653150356433	30.083637426947433	19.698156211320693
12-13	24.946182073331244	24.499499123591463	29.930778579914406	20.62354022316289
14-15	22.19848711352724	25.6646103966007	28.12434022137352	24.012562268498545
16-17	21.244594506633465	27.884050811220394	28.908169617747543	21.963185064398598
18-19	21.168987450575898	26.645970715228717	31.34173188394912	20.843309950246265
20-21	21.878499218144587	26.309397102603565	31.136388330367755	20.675715348884097
22-23	21.568420952491266	26.304824109381265	31.10882197772779	21.01793296039968
24-25	21.169775522173232	26.36735733429201	31.37372125013811	21.089145893396648
26-27	21.115367822599165	26.44400879633833	31.204419398943585	21.236203982118916
28-29	21.230354642883608	26.45212578102416	31.165642881606114	21.151876694486116
30-31	21.689403176017883	26.38771986484107	31.34533874436386	20.57753821477719
32-33	21.433443897566992	26.540339811018782	31.23936703158483	20.7868492598294
34-35	21.65414746113049	26.6646226011996	31.00122376875377	20.68000616891614
36-37	21.559481129871084	26.796484341097692	30.73190597314854	20.912128555882685
38-39	21.68986331038037	27.077032618840658	30.38394801601767	20.849156054761302
40-41	21.789923924861572	26.616801158769672	30.746019733668582	20.847255182700174
42-43	21.377725497501412	27.048115715013736	30.48909934537816	21.085059442106687
44-45	21.35618138835935	27.288823992160317	30.144613880321952	21.21038073915838
46-47	21.48810169599028	26.90704183976806	29.958355518686602	21.646500945555054
48-49	21.39893620306247	27.115471891101272	29.940207114423682	21.545384791412577
50-51	21.349898560654555	27.545351440805906	29.65811059934755	21.44663939919199
52-53	21.464567436427817	27.493453466745915	29.282001903080427	21.75997719374584
54-55	21.345126994557557	27.412901600853644	29.62728423042906	21.614687174159744
56-57	21.55463567122709	27.59956757536261	29.080556864390612	21.76523988901969
58-59	21.550529763334378	27.61236889440267	29.269630768783976	21.56747057347897
60-61	21.840814446874525	27.64885432730401	29.41570584010406	21.094625385717404
62-63	21.728398977118708	27.530345923497563	29.507104084180725	21.234151015203008
64-65	21.72354722930817	27.29616043475002	29.209291549945327	21.771000785996478
66-67	21.46013016650042	27.64510867460645	29.067335926514946	21.827425232378182
68-69	21.423000855998602	27.467606262010353	29.39864429051604	21.710748591475003
70-71	21.499441941652485	27.291131203548343	29.32414268734929	21.885284167449885
72-73	21.273411778216822	27.95817511819814	28.761575271900792	22.006837831684244
74-75	21.446666129202672	27.823893412544177	28.78550329413685	21.9439371641163
76-77	21.28434489967902	27.558105318711956	29.208676753816647	21.948873027792377
78-79	21.16577566529642	27.63632187423109	29.102843470094502	22.095058990377986
80-81	21.240189150786012	27.897854836736354	28.662977143640195	22.19897886883744
82-83	21.383111536067435	27.953232695915542	28.812049915527133	21.851605852489893
84-85	21.223818694417105	28.128000758578413	28.605517856642038	22.042662690362448
86-87	21.010632748437853	28.190680032176846	28.652333313802874	22.146353905582423
88-89	20.952590170020493	27.865552738536486	28.8878591226347	22.29399796880832
90-91	21.440346380593926	27.970844007190998	28.779914626947008	21.808894985268072
92-93	21.168471655728553	28.126110171777796	28.632665694389907	22.07275247810374
94-95	20.923045536396696	27.82911688900568	28.656690154365705	22.591147420231923
96-97	21.08950941461079	27.97019802766925	28.65056132434217	22.289731233377783
98-99	21.09959208583759	27.657234583695857	28.743842726354302	22.49933060411226
100	20.007912673465334	28.36973886508224	28.93921764693117	22.683130814521256
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1461.0
1	1264.5
2	3881.5
3	5795.5
4	3382.0
5	2045.5
6	2789.0
7	4937.5
8	7506.5
9	8324.0
10	6930.5
11	5428.0
12	5373.5
13	5723.0
14	6153.5
15	6549.0
16	6805.0
17	7219.0
18	7597.0
19	7886.0
20	8451.5
21	9372.0
22	10542.5
23	12313.5
24	14562.0
25	17388.0
26	20816.5
27	24804.5
28	28999.5
29	33745.0
30	39571.5
31	45942.5
32	52754.0
33	60501.5
34	69537.5
35	77879.5
36	87106.0
37	95034.5
38	97988.5
39	98219.0
40	95625.0
41	93927.0
42	93574.0
43	93339.0
44	94595.5
45	97474.5
46	99422.0
47	98820.0
48	99581.5
49	101663.0
50	103990.0
51	102133.5
52	96282.0
53	91567.5
54	87493.5
55	81441.0
56	74398.5
57	68751.5
58	62445.5
59	54074.0
60	43788.5
61	34331.5
62	27632.5
63	21451.5
64	15778.5
65	11974.0
66	9055.5
67	6873.5
68	5284.5
69	3966.0
70	3052.0
71	2358.0
72	1895.5
73	1585.0
74	1283.0
75	944.0
76	640.5
77	433.0
78	303.5
79	212.5
80	147.5
81	97.0
82	74.0
83	59.5
84	41.0
85	25.0
86	20.5
87	14.5
88	8.5
89	5.5
90	3.5
91	2.0
92	2.5
93	2.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.24774709367824324
2	0.07580620449249398
3	0.0016356248437394121
4	0.0011349233609620412
5	2.336606919627732E-4
6	5.00701482777371E-4
7	0.0
8	6.676019770364948E-4
9	0.0
10-11	3.004208896664227E-4
12-13	0.0016022447448875874
14-15	1.669004942591237E-5
16-17	6.676019770364948E-5
18-19	0.0014019641517766391
20-21	0.0012851338057952525
22-23	1.669004942591237E-5
24-25	0.0
26-27	1.669004942591237E-4
28-29	0.0017023850414430617
30-31	0.0021530163759426956
32-33	0.003421460132312036
34-35	0.004439553147292691
36-37	0.0019026656345540102
38-39	0.0035049103794415975
40-41	0.006876300363475896
42-43	0.013468869886711282
44-45	0.008962556541714944
46-47	0.0067427799680685975
48-49	0.006892990412901809
50-51	0.00549102626112517
52-53	0.004255962603607655
54-55	0.005891587447347067
56-57	0.004356102900163129
58-59	0.002420057166757294
60-61	0.008094673971567498
62-63	0.011249093313064937
64-65	0.00789439337845655
66-67	0.008311644614104361
68-69	0.015521745966098503
70-71	0.004756664086385026
72-73	0.01345217983728537
74-75	0.008261574465826624
76-77	0.0045897635921259014
78-79	0.015955687251172225
80-81	0.006592569523235387
82-83	0.015238015125857993
84-85	0.024067051272165636
86-87	0.015872237004042663
88-89	0.01705723051328244
90-91	0.03141067301956708
92-93	0.025535775621645926
94-95	0.02990856857123497
96-97	0.01865947525817003
98-99	0.01865947525817003
100	0.020094819508798493
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2995797.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.47328599596905
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.77120933707184	47.96149717171298
2	15.214614135850876	19.010138804546507
3	4.090707880802147	7.6667989008995105
4	1.4718099429226978	3.677952139836824
5	0.7271115224162833	2.271252304543846
6	0.3874286925701651	1.4522366108388176
7	0.27220339407286276	1.1903808340891193
8	0.19099822117361268	0.9545829196880364
9	0.13796613952310063	0.7757278282967791
>10	0.6688801863016174	7.351302849181785
>50	0.043576058864886275	1.8662733877941289
>100	0.021264626127649906	2.5049607372592657
>500	0.0015927587873236892	0.675439093147644
>1k	3.18551757460221E-4	0.3815812955766977
>5k	1.274207029840884E-4	0.5880738126781796
>10k+	1.9113105447613257E-4	1.6718013099100901
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17000	0.5674616804810206	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14217	0.4745648653763923	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10786	0.36003774621578166	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9643	0.32188429322814593	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5132	0.17130666730756455	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.676019770364948E-5
2	0.0	0.0	0.0	0.0	6.676019770364948E-5
3	0.0	0.0	0.0	0.0	6.676019770364948E-5
4	0.0	0.0	0.0	0.0	6.676019770364948E-5
5	0.0	0.0	0.0	0.0	6.676019770364948E-5
6	0.0	0.0	0.0	0.0	6.676019770364948E-5
7	0.0	0.0	0.0	0.0	6.676019770364948E-5
8	0.0	0.0	0.0	0.0	1.0014029655547421E-4
9	0.0	0.0	0.0	3.338009885182474E-5	1.0014029655547421E-4
10-11	0.0	0.0	0.0	3.338009885182474E-5	1.0014029655547421E-4
12-13	0.0	0.0	0.0	3.338009885182474E-5	1.0014029655547421E-4
14-15	0.0	0.0	0.0	3.338009885182474E-5	1.0014029655547421E-4
16-17	0.0	0.0	0.0	3.338009885182474E-5	1.0014029655547421E-4
18-19	0.0	0.0	0.0	3.338009885182474E-5	1.0014029655547421E-4
20-21	0.0	0.0	0.0	1.1683034598138658E-4	1.1683034598138658E-4
22-23	0.0	0.0	0.0	2.169706425368608E-4	1.8359054368503606E-4
24-25	0.0	0.0	0.0	5.173915322032835E-4	2.3366069196277317E-4
26-27	0.0	0.0	0.0	0.001084853212684304	2.3366069196277317E-4
28-29	0.0	0.0	0.0	0.002036186029961309	2.5035074138868556E-4
30-31	0.0	0.0	0.0	0.005958347645050716	2.670407908145979E-4
32-33	0.0	0.0	0.0	0.014036331567192303	2.8373084024051025E-4
34-35	0.0	0.0	0.0	0.024968313941164905	3.0042088966642264E-4
36-37	0.0	0.0	0.0	0.043460888705075806	3.0042088966642264E-4
38-39	0.0	0.0	0.0	0.07435417019243962	3.0042088966642264E-4
40-41	0.0	0.0	0.0	0.1125243132295012	3.0042088966642264E-4
42-43	0.0	0.0	0.0	0.1480407384078427	3.671810873700721E-4
44-45	0.0	0.0	0.0	0.18479222724370176	3.671810873700721E-4
46-47	0.0	0.0	0.0	0.22598326922685347	3.671810873700721E-4
48-49	0.0	0.0	0.0	0.27286561806424137	3.671810873700721E-4
50-51	0.0	0.0	0.0	0.32261865540288615	3.671810873700721E-4
52-53	0.0	0.0	0.0	0.3734565459542152	3.838711367959845E-4
54-55	0.0	1.669004942591237E-5	0.0	0.4336575542334811	4.0056118622189686E-4
56-57	0.0	3.338009885182474E-5	0.0	0.49619516943237474	4.0056118622189686E-4
58-59	0.0	3.338009885182474E-5	0.0	0.568913714781075	4.0056118622189686E-4
60-61	0.0	3.338009885182474E-5	0.0	0.6421162715631266	4.0056118622189686E-4
62-63	0.0	3.338009885182474E-5	0.0	0.7206763342108962	4.339412850737216E-4
64-65	0.0	3.338009885182474E-5	0.0	0.7947801536619471	4.339412850737216E-4
66-67	0.0	3.338009885182474E-5	0.0	0.8778799097535648	4.339412850737216E-4
68-69	0.0	3.338009885182474E-5	0.0	0.976885282948077	4.339412850737216E-4
70-71	0.0	3.338009885182474E-5	0.0	1.0850368032279891	4.339412850737216E-4
72-73	0.0	3.338009885182474E-5	0.0	1.1803536754993746	4.339412850737216E-4
74-75	0.0	3.338009885182474E-5	0.0	1.2753868169305198	4.339412850737216E-4
76-77	0.0	3.338009885182474E-5	0.0	1.3802503974735272	4.339412850737216E-4
78-79	0.0	3.338009885182474E-5	0.0	1.5032226816436496	4.339412850737216E-4
80-81	0.0	3.338009885182474E-5	0.0	1.6175161401122975	4.339412850737216E-4
82-83	0.0	3.338009885182474E-5	0.0	1.741773558088215	4.50631334499634E-4
84-85	0.0	3.338009885182474E-5	0.0	1.8686846939228525	4.6732138392554634E-4
86-87	0.0	3.338009885182474E-5	0.0	1.9997015819162647	4.6732138392554634E-4
88	0.0	3.338009885182474E-5	0.0	2.106517898242104	4.6732138392554634E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	4360	0.0	60.6285	2
GTACCTG	4810	0.0	55.10831	1
ACCTGGG	4995	0.0	52.211224	3
TAACGCA	455	0.0	50.603878	4
TATAACG	460	0.0	48.058147	2
GGTATCA	8170	0.0	44.048218	1
TACCGTA	295	0.0	41.4157	7
CCTGGGG	4120	0.0	38.549488	4
GTATCAA	13085	0.0	34.17122	1
ATAACGC	685	0.0	33.613358	3
ACCGTAT	365	0.0	32.185535	8
TATCACG	385	0.0	31.764326	2
GTATAAC	975	0.0	28.057516	1
ATCAACG	16020	0.0	27.16157	3
TCAACGC	16060	0.0	27.09347	4
CAACGCA	16240	0.0	26.79362	5
CTGGGGG	2790	0.0	26.779306	5
TATCAAC	16455	0.0	26.61208	2
AACGCAG	16855	0.0	26.178844	6
GTACCGT	450	0.0	26.106049	6
>>END_MODULE