FastQCFastQC Report
Mon 6 Jun 2016
ERR1211649_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211649_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5744014
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191920.33412174831050206No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163370.2844178304579341No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110300.19202599436561263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97450.045.5855641
GTATCAA164950.037.3950161
GTACCTG58300.032.1938441
TACCTGG55950.031.6998372
GTACATG218900.030.9792861
TCAACGC197950.030.2655244
TACATGG222750.030.0540472
CAACGCA200400.029.8449765
ATCAACG201450.029.7617193
AACGCAG207550.028.9267446
TATCAAC212050.028.5532822
ACATGGG226750.028.5339093
CATGGGG147300.027.8487054
ATGGGGG92400.026.5423325
ACGCAGA235850.025.2570347
ACCTGGG68400.025.1419873
CGCAGAG242150.024.4647588
GCAGAGT268800.021.2878679
TAACGCA5750.020.4298744
TATAACG5350.020.225062
Produced by FastQC (version 0.11.5)