FastQCFastQC Report
Mon 6 Jun 2016
ERR1211649_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211649_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5744014
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224200.3903193829262951No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192870.3357756440008677No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139130.24221737621113038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109150.046.096311
GTATCAA179950.037.3412171
TACCTGG57700.034.4776952
GTACCTG59550.033.5744971
TCAACGC227150.029.1265564
ATCAACG229050.028.8649353
CAACGCA229600.028.7743235
TATCAAC235750.028.1680432
AACGCAG237550.027.891926
GTACATG229700.027.7549951
TACATGG235650.026.8627992
ACCTGGG72300.026.7772353
CATGGGG147550.025.9547964
ACATGGG236850.025.7716713
ACGCAGA272300.024.2289077
ATGGGGG89000.023.7582345
CGCAGAG280600.023.5122288
TGGGGGG77050.021.1017176
TAACGCA5400.020.8841464
GCAGAGT311000.020.8360049
Produced by FastQC (version 0.11.5)