FastQCFastQC Report
Mon 6 Jun 2016
ERR1211648_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211648_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4364105
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196220.44962254574534755No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158380.3629151910872905No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110520.2532478022412385No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110130.2523541482159572No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51050.11697702048873711No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG67050.063.0782172
GTACCTG70400.060.5977971
ACCTGGG79100.052.398943
GGTATCA87550.045.4393921
TATAACG5650.041.633222
TAACGCA5650.039.0912744
GTATCAA156050.039.0412331
CCTGGGG64750.035.7071954
TCAACGC187900.031.2617744
ATCAACG188950.031.013443
CAACGCA191050.030.8165935
AACGCAG197450.029.9805286
TATCAAC197800.029.7542842
TATCACG5300.029.2925072
GTACATG170000.028.7311151
GTATAAC13950.027.0630551
TACATGG178250.026.8680842
ACATGGG175750.026.6314263
ACGCAGA220950.026.601077
CGCAGAG225000.026.0187428
Produced by FastQC (version 0.11.5)