FastQCFastQC Report
Mon 6 Jun 2016
ERR1211648_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211648_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4364105
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226870.5198545864501427No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184250.4221942414309463No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140830.3227007599496346No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113020.258976353685349No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52660.12066620761874428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG72300.062.6450652
GTACCTG75550.061.040121
ACCTGGG80750.055.224183
TATAACG7100.045.7077262
GGTATCA99300.044.6364441
TAACGCA7400.042.5440374
CCTGGGG65450.038.122424
GTATCAA172650.036.870431
TCAACGC212700.029.2051584
ATCAACG213950.029.1010863
CAACGCA215900.028.8583565
TATCAAC223700.028.0050982
AACGCAG226300.027.8640166
GTACATG179450.027.3275621
ATAACGC12750.026.9040853
TATCACG5450.026.7524812
CTGGGGG45500.025.9204795
ACATGGG180750.025.607283
TACATGG188750.025.3913692
ACGCAGA251150.025.0131827
Produced by FastQC (version 0.11.5)