##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211648_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4364105 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.995271195353915 33.0 31.0 34.0 30.0 34.0 2 32.24781209434695 34.0 31.0 34.0 30.0 34.0 3 32.33610419547651 34.0 31.0 34.0 30.0 34.0 4 35.992377360306406 37.0 35.0 37.0 35.0 37.0 5 35.89546676810022 37.0 35.0 37.0 35.0 37.0 6 35.92776571599446 37.0 35.0 37.0 35.0 37.0 7 35.83822731121272 37.0 35.0 37.0 35.0 37.0 8 35.8178574988457 37.0 35.0 37.0 35.0 37.0 9 37.446668675478705 39.0 37.0 39.0 34.0 39.0 10-11 37.39968206539485 39.0 37.0 39.0 34.0 39.0 12-13 37.306628850589064 39.0 37.0 39.0 34.0 39.0 14-15 38.49474542890238 40.0 38.0 41.0 34.0 41.0 16-17 38.43557751245673 40.0 38.0 41.0 34.0 41.0 18-19 38.44957339477396 40.0 38.0 41.0 34.0 41.0 20-21 38.383657932153326 40.0 38.0 41.0 34.0 41.0 22-23 38.25540803899081 40.0 38.0 41.0 34.0 41.0 24-25 38.14050463955381 40.0 37.0 41.0 33.0 41.0 26-27 37.921269080372724 40.0 37.0 41.0 33.0 41.0 28-29 37.93006790624882 40.0 37.0 41.0 33.0 41.0 30-31 37.785557519812194 40.0 37.0 41.0 33.0 41.0 32-33 37.561186428832485 40.0 36.5 41.0 32.5 41.0 34-35 37.3869320284457 40.0 36.0 41.0 32.0 41.0 36-37 37.26307536138567 40.0 36.0 41.0 31.0 41.0 38-39 37.175457167047995 40.0 36.0 41.0 31.0 41.0 40-41 37.01580988541751 39.0 36.0 41.0 31.0 41.0 42-43 36.82131124251136 39.0 35.0 41.0 30.0 41.0 44-45 36.60512636611631 39.0 35.0 41.0 30.0 41.0 46-47 36.329460450653684 39.0 35.0 41.0 29.5 41.0 48-49 36.04583540038565 39.0 35.0 40.0 28.0 41.0 50-51 35.830683725529056 39.0 35.0 40.0 27.0 41.0 52-53 35.62987657263058 38.5 34.5 40.0 26.5 41.0 54-55 35.407883059642245 38.0 34.0 40.0 26.0 41.0 56-57 35.1949668947012 38.0 34.0 40.0 26.0 41.0 58-59 34.93159765862646 38.0 34.0 40.0 25.0 41.0 60-61 34.61164339538118 37.5 33.0 40.0 25.0 41.0 62-63 34.2577472356875 37.0 33.0 40.0 23.5 41.0 64-65 33.937503451452244 36.5 32.5 39.5 23.0 41.0 66-67 33.57716106738953 36.0 32.0 39.0 22.5 40.5 68-69 33.178474165951556 35.5 31.5 39.0 22.0 40.0 70-71 32.802904375582166 35.0 31.0 38.0 21.5 40.0 72-73 32.38543309567483 35.0 31.0 37.5 20.5 40.0 74-75 31.979597764031798 35.0 30.5 37.0 20.0 39.0 76-77 30.50737264570857 33.5 28.5 35.5 19.5 38.0 78-79 31.371863303014017 34.0 30.0 36.0 20.0 38.5 80-81 31.330849968091968 34.0 30.0 36.0 20.0 37.5 82-83 31.05155432786333 34.0 30.0 35.0 19.5 37.0 84-85 30.721078892464778 34.0 29.5 35.0 18.5 36.5 86-87 30.422455807089882 34.0 29.0 35.0 18.0 36.0 88-89 30.138635871501716 34.0 29.0 35.0 18.0 36.0 90-91 29.849067334539384 34.0 29.0 35.0 17.0 35.5 92-93 29.60550823135557 34.0 29.0 35.0 15.5 35.0 94-95 29.38239272428138 33.5 27.5 35.0 15.0 35.0 96-97 29.14564429590947 33.0 27.0 35.0 12.5 35.0 98-99 28.931662391257774 33.0 27.0 35.0 11.0 35.0 100 27.17755003603259 31.0 24.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18230449329989895 1101 2 0.08360497228745345 1101 3 0.11088376822781498 1101 4 0.11505871009796209 1101 5 0.08387468110751684 1101 6 0.07697783217167853 1101 7 0.09714891268527737 1101 8 0.05423570983632686 1101 9 0.14515355563035826 1101 10-11 0.08568153195187023 1101 12-13 0.04043748964123495 1101 14-15 0.04102007655573914 1101 16-17 0.12226045397175511 1101 18-19 0.03134927213573491 1101 20-21 0.0073180699112427305 1101 22-23 -0.04998647577153292 1101 24-25 -0.13634176992641756 1101 26-27 -0.09857908435064644 1101 28-29 -0.01408136329069265 1101 30-31 0.03640199085081974 1101 32-33 -0.0045874567817136835 1101 34-35 -0.014748021937286637 1101 36-37 -0.061667926470782675 1101 38-39 -0.10237973985772442 1101 40-41 -0.10781297816840407 1101 42-43 -0.049996308053870564 1101 44-45 -0.10054835969370401 1101 46-47 -0.21778903319940213 1101 48-49 -0.1394886447252901 1101 50-51 -0.32807789626915707 1101 52-53 -0.2664074964318388 1101 54-55 -0.3740056839699122 1101 56-57 -0.3767665373515072 1101 58-59 -0.4705879095152241 1101 60-61 -0.5647282598069481 1101 62-63 -0.6679401565691165 1101 64-65 -0.5734823034608993 1101 66-67 -0.6494940766656256 1101 68-69 -0.6492003645145275 1101 70-71 -0.7216933480939147 1101 72-73 -0.7760391632819221 1101 74-75 -0.8203797577059255 1101 76-77 -0.5320371770773171 1101 78-79 -0.5420464116203441 1101 80-81 -0.49444304145040263 1101 82-83 -0.7452856185731918 1101 84-85 -0.7980665047005964 1101 86-87 -0.8811844756701639 1101 88-89 -0.9642519478641418 1101 90-91 -1.1163752872144599 1101 92-93 -1.0674242402355816 1101 94-95 -1.099113107327586 1101 96-97 -1.2602136122883216 1101 98-99 -1.2396555830325902 1101 100 -1.2834197522188688 1102 1 0.23237419636567935 1102 2 0.10811214179065587 1102 3 0.04206102129698763 1102 4 0.060829399402273054 1102 5 0.052450548137841224 1102 6 0.0871190087065088 1102 7 0.07377801446078536 1102 8 0.022605082321824455 1102 9 0.04719859191732212 1102 10-11 0.04531166467751291 1102 12-13 0.09842095306665755 1102 14-15 0.13219894251898268 1102 16-17 0.1563148591152128 1102 18-19 0.11432568965016543 1102 20-21 0.05585302970544603 1102 22-23 -0.004368533626966098 1102 24-25 0.06349480904123084 1102 26-27 0.027176670982555606 1102 28-29 -0.027517369512935375 1102 30-31 0.04106604790526802 1102 32-33 0.04531266139218104 1102 34-35 -0.03130693656877526 1102 36-37 -0.010145568671653393 1102 38-39 0.01595095362162624 1102 40-41 -0.027625969610191703 1102 42-43 -0.12902299893075053 1102 44-45 -0.13332182136972648 1102 46-47 -0.12409947078722894 1102 48-49 -0.20692510079377513 1102 50-51 -0.13296562162017267 1102 52-53 -0.1604908895661623 1102 54-55 -0.2179290162678882 1102 56-57 -0.18191696177576233 1102 58-59 -0.23396269716658225 1102 60-61 -0.21040887485722237 1102 62-63 -0.20243584995291286 1102 64-65 -0.22831793002298895 1102 66-67 -0.31267060225236776 1102 68-69 -0.46890256399381514 1102 70-71 -0.3779606019128252 1102 72-73 -0.5350151656021396 1102 74-75 -0.4881501437525415 1102 76-77 -0.4024096621543727 1102 78-79 -0.4129488626103104 1102 80-81 -0.24046895339184715 1102 82-83 -0.489618438006552 1102 84-85 -0.535879703844504 1102 86-87 -0.47528219689024453 1102 88-89 -0.5387786800084271 1102 90-91 -0.5340767629915426 1102 92-93 -0.669006273015551 1102 94-95 -0.5198685287413163 1102 96-97 -0.7008475069633988 1102 98-99 -0.8614545763178647 1102 100 -0.8777406114545876 1103 1 0.27702686787461417 1103 2 0.214210057604177 1103 3 0.19278466722236942 1103 4 0.12653946686352668 1103 5 0.12121577002719164 1103 6 0.10750825801142128 1103 7 0.13327808989669876 1103 8 0.13540871186683745 1103 9 0.16608020064202123 1103 10-11 0.16080404565042272 1103 12-13 0.11449014935089252 1103 14-15 0.15522979580691754 1103 16-17 0.13506348510395583 1103 18-19 0.11930445982927296 1103 20-21 0.09253643596016303 1103 22-23 0.12085238582903912 1103 24-25 0.021885168331699845 1103 26-27 0.03730663628203956 1103 28-29 0.15502676049546693 1103 30-31 0.1843565623781842 1103 32-33 0.2010972487577405 1103 34-35 0.10671249356679624 1103 36-37 0.08363743453976014 1103 38-39 0.1530314760692022 1103 40-41 0.021257848309069516 1103 42-43 0.027868879854516138 1103 44-45 0.05563436114207576 1103 46-47 0.07645767730424069 1103 48-49 0.1248878396980686 1103 50-51 0.034069693163104375 1103 52-53 -0.1073260836519836 1103 54-55 -0.02646863089813678 1103 56-57 -0.09234220478716537 1103 58-59 -0.10673329655879371 1103 60-61 -0.1511095128294997 1103 62-63 -0.21075265922937803 1103 64-65 -0.38661749680532864 1103 66-67 -0.3340849717159031 1103 68-69 -0.28986081560491783 1103 70-71 -0.3444145213472751 1103 72-73 -0.3182244787042734 1103 74-75 -0.37343906329475374 1103 76-77 -0.18086785988267096 1103 78-79 -0.2289606737187846 1103 80-81 -0.1846194372593395 1103 82-83 -0.18878541000905003 1103 84-85 -0.20446414895948806 1103 86-87 -0.0906587298557362 1103 88-89 -0.28045244124299273 1103 90-91 -0.24721419644628995 1103 92-93 -0.2612482283143862 1103 94-95 -0.369291713429984 1103 96-97 -0.44765764554994547 1103 98-99 -0.4879026764105454 1103 100 -0.39748057309003926 1104 1 0.30314375375484204 1104 2 0.1292197508102646 1104 3 0.13983592300480296 1104 4 0.09714422835549641 1104 5 0.12487239411204598 1104 6 0.14437711602565173 1104 7 0.18584580442784215 1104 8 0.1293634611437824 1104 9 0.15259088287035638 1104 10-11 0.1460260437117782 1104 12-13 0.1616155818018612 1104 14-15 0.175617346735649 1104 16-17 0.10725225832234031 1104 18-19 0.1710626340390391 1104 20-21 0.1435786526309002 1104 22-23 0.09891185325435004 1104 24-25 0.042522339018816524 1104 26-27 0.12430966245369746 1104 28-29 0.05974057449623871 1104 30-31 0.1674103951349224 1104 32-33 0.04651533722597634 1104 34-35 0.16583799694365808 1104 36-37 0.13308421253381653 1104 38-39 0.1285870622717482 1104 40-41 0.1938502095898329 1104 42-43 0.15480308820328048 1104 44-45 0.11039033664899733 1104 46-47 0.26635250995853 1104 48-49 0.20814085601890753 1104 50-51 0.12399240363571096 1104 52-53 0.003940199752136664 1104 54-55 0.012325175694812174 1104 56-57 0.16945198401757722 1104 58-59 0.018303841029570833 1104 60-61 0.04536576555464933 1104 62-63 0.015121502465909487 1104 64-65 0.12253332317686016 1104 66-67 0.07970292438552917 1104 68-69 -0.026723172078483515 1104 70-71 -0.002090772204297764 1104 72-73 0.1083448553015316 1104 74-75 0.023974702634017575 1104 76-77 0.0541963280551947 1104 78-79 0.18388006611713692 1104 80-81 0.1366010004322611 1104 82-83 0.11847326462233099 1104 84-85 0.09174121697874327 1104 86-87 -0.029320239946915905 1104 88-89 0.022707011997141535 1104 90-91 0.017132241103457346 1104 92-93 -0.002333039927318481 1104 94-95 -0.06563971923014478 1104 96-97 -0.17806515423837155 1104 98-99 -0.07286116505845897 1104 100 -0.06831170640708706 1105 1 0.3237999826709874 1105 2 0.15925308811232242 1105 3 0.17074039575949485 1105 4 0.11324634368668285 1105 5 0.0864541300329904 1105 6 0.10688455738771552 1105 7 0.10810242227917399 1105 8 0.13806588603564052 1105 9 0.14037712726453577 1105 10-11 0.19912859560287188 1105 12-13 0.2610376360146702 1105 14-15 0.24197494883113535 1105 16-17 0.26337699539421067 1105 18-19 0.25452098225509445 1105 20-21 0.14578427234753377 1105 22-23 0.08763670145056324 1105 24-25 0.15883479685109592 1105 26-27 0.3079882749869327 1105 28-29 0.20809107257140624 1105 30-31 0.3487707753389131 1105 32-33 0.3229098484773161 1105 34-35 0.30587202877283914 1105 36-37 0.33465880474717125 1105 38-39 0.2296189311450263 1105 40-41 0.25162940949458346 1105 42-43 0.29067690254625944 1105 44-45 0.2808233247031353 1105 46-47 0.29725152571437263 1105 48-49 0.3431608980874117 1105 50-51 0.423060249014128 1105 52-53 0.27709771850437903 1105 54-55 0.2266051495012249 1105 56-57 0.2258962035675225 1105 58-59 0.2934343129111454 1105 60-61 0.29039840832958674 1105 62-63 0.21990778654734555 1105 64-65 0.21317903496794344 1105 66-67 0.16291719592579312 1105 68-69 0.2177682356287889 1105 70-71 0.2881808871202267 1105 72-73 0.19079117827649128 1105 74-75 0.11707407954397198 1105 76-77 0.1347252600709119 1105 78-79 0.25905766662513585 1105 80-81 0.2587400447629342 1105 82-83 0.17912483028491266 1105 84-85 0.16750618899922287 1105 86-87 0.15226238893747635 1105 88-89 0.07835450971512259 1105 90-91 -0.022446875458040694 1105 92-93 0.08893328146015023 1105 94-95 0.04589588024598257 1105 96-97 0.10766032895407562 1105 98-99 -0.020802884310754166 1105 100 -0.007011750063075084 1106 1 0.26453737360522567 1106 2 0.16481219052181473 1106 3 0.14061696524235856 1106 4 0.13519368472851312 1106 5 0.09561269804982686 1106 6 0.11825931797508105 1106 7 0.11981295170623696 1106 8 0.13185593592805134 1106 9 0.07965853465617556 1106 10-11 0.13045559189050948 1106 12-13 0.20575843949530537 1106 14-15 0.18867489681061045 1106 16-17 0.12675750188495272 1106 18-19 0.15968520159254496 1106 20-21 0.14258264552385924 1106 22-23 0.14980418389362882 1106 24-25 0.16025708528125904 1106 26-27 0.2292723543418802 1106 28-29 0.16548064718539024 1106 30-31 0.26617311862707993 1106 32-33 0.2830854768616078 1106 34-35 0.2602911132116077 1106 36-37 0.27883723514607794 1106 38-39 0.24784207554927207 1106 40-41 0.4099760435301931 1106 42-43 0.3731037191266182 1106 44-45 0.26653837045754614 1106 46-47 0.26867068109022085 1106 48-49 0.3425904457453015 1106 50-51 0.44343781161609996 1106 52-53 0.24433779322475857 1106 54-55 0.24988226519960222 1106 56-57 0.37366109444265305 1106 58-59 0.5431845552795025 1106 60-61 0.4862926293222287 1106 62-63 0.3598589581914311 1106 64-65 0.3758383478241072 1106 66-67 0.43208025662428895 1106 68-69 0.35652411749490653 1106 70-71 0.36608989849380436 1106 72-73 0.2840492000778241 1106 74-75 0.39490742470014695 1106 76-77 0.4259689644051683 1106 78-79 0.3904609202724849 1106 80-81 0.3726475546350123 1106 82-83 0.31677734500435406 1106 84-85 0.34009491946196846 1106 86-87 0.3701695677698531 1106 88-89 0.4661007204928289 1106 90-91 0.41259899718625803 1106 92-93 0.4018464899442229 1106 94-95 0.36794519333860265 1106 96-97 0.30416608668030776 1106 98-99 0.2929077751676239 1106 100 0.2433210630650109 1107 1 0.1902404512172886 1107 2 0.14768341909125127 1107 3 0.13262333701608497 1107 4 0.09529886056641601 1107 5 0.031096882937085013 1107 6 0.04680049924750307 1107 7 0.03216601070560898 1107 8 0.028699338062175173 1107 9 0.05287299246018051 1107 10-11 0.049212264585037246 1107 12-13 0.15563490607089392 1107 14-15 0.12079769706532772 1107 16-17 0.15571350036570664 1107 18-19 0.10587914387243558 1107 20-21 0.12544395362708372 1107 22-23 0.1576063471221545 1107 24-25 0.11012826094153638 1107 26-27 0.16328812126950254 1107 28-29 0.17536506903007165 1107 30-31 0.11782808859168625 1107 32-33 0.09328154306607672 1107 34-35 0.17899653016407768 1107 36-37 0.17457620594966983 1107 38-39 0.1861738121599501 1107 40-41 0.2184213364106995 1107 42-43 0.2298998998416053 1107 44-45 0.33396480311458987 1107 46-47 0.29778394258048024 1107 48-49 0.14502204649283357 1107 50-51 0.20991570598297926 1107 52-53 0.267916993502908 1107 54-55 0.30626410110942004 1107 56-57 0.30255109820297577 1107 58-59 0.24379598667994884 1107 60-61 0.1686486077730862 1107 62-63 0.20986750868849668 1107 64-65 0.3855527045338434 1107 66-67 0.3548056826846917 1107 68-69 0.3658496864100229 1107 70-71 0.33103545875449214 1107 72-73 0.311799389511485 1107 74-75 0.3105459531454322 1107 76-77 0.29343096901960664 1107 78-79 0.2946447115534312 1107 80-81 0.2976123133598243 1107 82-83 0.3364076800893585 1107 84-85 0.28480669685048454 1107 86-87 0.18468533564228196 1107 88-89 0.1220769038154863 1107 90-91 0.13073011362607545 1107 92-93 0.18889801401127215 1107 94-95 0.36642372450493 1107 96-97 0.3514469341337687 1107 98-99 0.2385157485769973 1107 100 0.4522380504934205 1108 1 0.2295853114733113 1108 2 0.14931333794594082 1108 3 0.08740058394942452 1108 4 0.08806779294156541 1108 5 -0.00700540599028443 1108 6 0.04965251601969811 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-0.12009437614672436 2316 42-43 -0.10437743013694245 2316 44-45 -0.4267698999648317 2316 46-47 -0.1683859545494002 2316 48-49 -0.24479884000120222 2316 50-51 0.009654123816766003 2316 52-53 -0.2068137703040236 2316 54-55 0.08409330776257207 2316 56-57 -0.0694419825567465 2316 58-59 -0.12870045071846192 2316 60-61 0.10233824765344224 2316 62-63 0.1347668466383638 2316 64-65 -0.23671531814805036 2316 66-67 -0.1689411443732709 2316 68-69 0.13474836008313673 2316 70-71 -0.09313354586946332 2316 72-73 -0.34411466829172355 2316 74-75 -0.09225361154918588 2316 76-77 -0.13559667268658515 2316 78-79 -0.17486020743449515 2316 80-81 0.029976229401182053 2316 82-83 0.041321240612766985 2316 84-85 0.2162773183760187 2316 86-87 0.24264213724248052 2316 88-89 0.29939797586168027 2316 90-91 0.32249484461254596 2316 92-93 0.31252562380249316 2316 94-95 0.38865732726000957 2316 96-97 0.4120893713985829 2316 98-99 0.39046766458866244 2316 100 0.1852795102694209 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 9.0 11 27.0 12 64.0 13 186.0 14 667.0 15 1724.0 16 4018.0 17 8579.0 18 16010.0 19 23027.0 20 29900.0 21 38337.0 22 47762.0 23 52294.0 24 53517.0 25 56940.0 26 64306.0 27 74958.0 28 89305.0 29 105853.0 30 124954.0 31 150055.0 32 181439.0 33 224280.0 34 293784.0 35 398945.0 36 562949.0 37 842793.0 38 799983.0 39 117427.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.357386358415496 19.739913074640032 13.14185557158478 22.76084499535969 2 15.253588183183497 21.561984234857647 40.62449190289575 22.55993567906311 3 16.8350311855314 29.410617631402808 30.72879896004691 23.025552223018884 4 11.375144647746932 17.77365062270139 38.99313710887822 31.85806762067346 5 11.697611601577968 38.0540666383141 35.32924115326519 14.919080606842746 6 28.551044608536746 38.33699008380928 19.85655608576846 13.25540922188551 7 26.62392403482501 32.82494348784 23.74551024780568 16.80562222952931 8 26.492348867797418 35.10421488247183 21.09164401955977 17.31179223017099 9 27.375097528588338 15.390532537599347 21.184251982938083 36.05011795087423 10-11 24.26413100365437 26.53846435867988 29.665506593353207 19.53189804431254 12-13 25.015049111546745 24.652106016322012 29.5941036355033 20.738741236627945 14-15 22.487227618773524 25.636728638914196 27.60415425480236 24.27188948750992 16-17 21.47620603059119 28.29312209769736 28.273232542016974 21.957439329694477 18-19 21.318910035527775 27.241183391438756 30.46355673473531 20.97634983829816 20-21 22.022128165450706 26.709858385515595 30.552991030540344 20.715022418493362 22-23 21.848606054231745 26.55647911272519 30.39528498916559 21.199629843877478 24-25 21.43803418044508 26.77321928776437 30.59581423767791 21.19293229411264 26-27 21.505594399024584 26.750814658794248 30.494842346538125 21.24874859564304 28-29 21.585841708906873 26.510877160660804 30.75471877538866 21.14856235504366 30-31 21.868766502834657 26.788882643377455 30.570106986145586 20.772243867642313 32-33 21.70362122685275 26.881265950238348 30.46620697746521 20.948905845443694 34-35 21.91524704811537 26.88881774978248 30.379852274578724 20.816082927523432 36-37 21.91472933852411 26.855985929491872 30.211079390878343 21.018205341105674 38-39 21.8754980430025 27.224910978125234 29.9682936730221 20.931297305850162 40-41 21.877989087467544 26.943093045265698 30.203996984272845 20.97492088299391 42-43 21.656754749636104 27.062930981042022 29.975397147966714 21.304917121355164 44-45 21.558989850310383 27.34527884920456 29.820489560592605 21.275241739892447 46-47 21.647604270288443 27.167403749048713 29.529688046753456 21.655303933909387 48-49 21.57672411985526 27.36382982453306 29.48240111544639 21.577044940165294 50-51 21.638152692762784 27.576446464931404 29.235782265426124 21.549618576879684 52-53 21.791827172553955 27.582287230806408 28.909884458676245 21.716001137963396 54-55 21.68562840118176 27.594643011618803 29.15643531230862 21.56329327489082 56-57 21.729273446349175 27.690091800259903 28.893628244809555 21.68700650858137 58-59 21.771149771299633 27.56291408331838 29.14001971124244 21.525916434139543 60-61 22.055762527330472 27.9415218990957 28.81469911330221 21.188016460271616 62-63 21.87565491744531 27.790900794007733 29.1048435101799 21.228600778367056 64-65 21.812943652469787 27.389616240613634 29.031932436495367 21.765507670421215 66-67 21.66232691852674 27.62036857208823 28.829665888090066 21.887638621294958 68-69 21.534336871300074 27.74085472686733 28.951450797068084 21.77335760476451 70-71 21.616696678144205 27.47686351109997 29.024051040746713 21.882388770009115 72-73 21.57487780219614 27.894902268684717 28.51544492925951 22.014774999859632 74-75 21.613158445025622 27.934998381547928 28.43746902741056 22.014374146015886 76-77 21.492672137623938 27.694395278343276 28.883540197603423 21.92939238642936 78-79 21.471402029028486 27.756568376408232 28.718199171328397 22.053830423234892 80-81 21.442457559157887 28.062384483099002 28.32718751815358 22.16797043958954 82-83 21.744578872113888 28.005045105498255 28.401865318555075 21.848510703832783 84-85 21.400315490129902 28.20649075389306 28.268523012271796 22.124670743705238 86-87 21.370208540286747 28.044382347120706 28.58650527575792 21.998903836834636 88-89 21.408802901069063 27.89981429436097 28.523565685778173 22.167817118791795 90-91 21.700187152384213 28.043082868508545 28.415164271200194 21.841565707907044 92-93 21.549091109119622 28.087249398924136 28.30642148425055 22.05723800770569 94-95 21.290088638841446 27.771394923439942 28.414809561667333 22.523706876051286 96-97 21.49748539410924 27.852251090227874 28.4364179654519 22.213845550210987 98-99 21.366889937327453 27.73178826919172 28.385454267100645 22.51586752638018 100 20.491574279429976 28.18451488077572 28.572001539220675 22.751909300573633 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2593.0 1 1997.5 2 4882.5 3 6740.0 4 3630.5 5 2368.0 6 3236.5 7 5844.0 8 9085.0 9 10254.0 10 8501.0 11 6556.5 12 6490.0 13 6973.5 14 7453.0 15 7829.5 16 8204.0 17 8666.0 18 9159.0 19 9652.0 20 10371.5 21 11511.0 22 13001.0 23 15139.5 24 18143.0 25 21711.5 26 26365.5 27 31960.5 28 37401.0 29 44363.5 30 53788.5 31 63010.5 32 74038.5 33 87784.0 34 101638.5 35 113080.5 36 126340.0 37 139619.5 38 147505.5 39 153032.5 40 151211.5 41 148251.0 42 147241.0 43 146080.0 44 148407.5 45 152634.5 46 153284.0 47 150244.5 48 149789.5 49 150653.5 50 151121.5 51 146175.5 52 137321.0 53 130856.5 54 125279.5 55 116772.0 56 106336.0 57 97671.5 58 88311.5 59 76252.5 60 61828.5 61 48652.5 62 38814.5 63 29940.0 64 22324.5 65 16747.5 66 12607.0 67 9555.0 68 7256.0 69 5566.0 70 4396.0 71 3354.5 72 2605.0 73 2072.5 74 1597.0 75 1232.5 76 909.5 77 612.0 78 438.0 79 315.5 80 215.5 81 157.5 82 114.0 83 84.5 84 65.0 85 45.5 86 26.0 87 17.5 88 12.5 89 7.0 90 3.5 91 1.5 92 2.0 93 1.5 94 0.5 95 1.5 96 2.5 97 2.5 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25079598222315913 2 0.07955812245580708 3 0.0014206807581394123 4 0.0012602813177043173 5 2.062278519879792E-4 6 6.874261732932641E-4 7 0.0 8 0.0010311392599398961 9 0.0 10-11 2.9788467509374777E-4 12-13 0.0013748523465865281 14-15 2.2914205776442135E-5 16-17 1.2602813177043173E-4 18-19 0.0012488242148160963 20-21 0.0016956512274567178 22-23 1.1457102888221067E-5 24-25 3.43713086646632E-5 26-27 1.8331364621153708E-4 28-29 0.0017300225361213811 30-31 0.001878964873668255 32-33 0.0032538172202547827 34-35 0.004537012743735543 36-37 0.0019133361823329184 38-39 0.0034256737635780994 40-41 0.007217974819579272 42-43 0.01343918168788331 44-45 0.008604284269054022 46-47 0.006370149205850914 48-49 0.006679490983832882 50-51 0.005740008546998755 52-53 0.0038610436733304996 54-55 0.006244121074080482 56-57 0.003987071805100931 58-59 0.002245592166091329 60-61 0.008844883429706664 62-63 0.01105610428713333 64-65 0.007538773700449463 66-67 0.008535541651724694 68-69 0.015490003104874884 70-71 0.005018211065040827 72-73 0.01341626748210687 74-75 0.008420970622842484 76-77 0.004880725830382175 78-79 0.01686485545146141 80-81 0.00677114780693865 82-83 0.015306689458663347 84-85 0.024117201579705348 86-87 0.016120143763727043 88-89 0.017139825920778718 90-91 0.03093417779819688 92-93 0.025091055325204135 94-95 0.029524954142945693 96-97 0.01826262200382438 98-99 0.018125136769165728 100 0.01966038855618735 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4364105.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.36529552222185 #Duplication Level Percentage of deduplicated Percentage of total 1 76.49561306025282 45.41184675475442 2 15.144425148639806 17.981065489263415 3 4.212100438352202 7.501577618761761 4 1.595400697298057 3.788457354858319 5 0.7802508754464681 2.3159911901175945 6 0.43645970729376293 1.5546335704222012 7 0.2611165719235764 1.0850883720594828 8 0.19156052940590085 0.9097637950859657 9 0.1397812577838827 0.7468340109127263 >10 0.6513898056712343 6.857632306916068 >50 0.047825412480660884 1.9854432736353769 >100 0.03814380865967281 4.546941998533255 >500 0.004040364282703211 1.6323360061927306 >1k 0.0016366032512771978 1.5035416786098768 >5k 5.114385160241243E-5 0.15988470506572183 >10k+ 2.0457540640964972E-4 2.0189618748111102 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22687 0.5198545864501427 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18425 0.4221942414309463 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14083 0.3227007599496346 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11302 0.258976353685349 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5266 0.12066620761874428 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 2.2914205776442135E-5 9 0.0 0.0 0.0 2.2914205776442135E-5 6.87426173293264E-5 10-11 0.0 0.0 0.0 2.2914205776442135E-5 6.87426173293264E-5 12-13 0.0 0.0 0.0 4.582841155288427E-5 6.87426173293264E-5 14-15 0.0 2.2914205776442135E-5 0.0 9.165682310576854E-5 8.019972021754748E-5 16-17 0.0 4.582841155288427E-5 0.0 9.165682310576854E-5 9.165682310576854E-5 18-19 0.0 4.582841155288427E-5 0.0 9.165682310576854E-5 9.165682310576854E-5 20-21 0.0 4.582841155288427E-5 0.0 1.031139259939896E-4 1.031139259939896E-4 22-23 0.0 4.582841155288427E-5 0.0 2.1768495487620027E-4 1.2602813177043173E-4 24-25 0.0 4.582841155288427E-5 0.0 5.041125270817269E-4 1.374852346586528E-4 26-27 0.0 4.582841155288427E-5 0.0 9.165682310576853E-4 1.4894233754687386E-4 28-29 0.0 4.582841155288427E-5 0.0 0.0017300225361213811 1.6039944043509495E-4 30-31 0.0 4.582841155288427E-5 0.0 0.005018211065040827 1.6039944043509495E-4 32-33 0.0 4.582841155288427E-5 0.0 0.011674787843097268 1.8331364621153708E-4 34-35 0.0 4.582841155288427E-5 0.0 0.021390411092308735 2.062278519879792E-4 36-37 0.0 4.582841155288427E-5 0.0 0.03582636073146728 2.062278519879792E-4 38-39 0.0 4.582841155288427E-5 0.0 0.06181107008195265 2.062278519879792E-4 40-41 0.0 4.582841155288427E-5 0.0 0.0934326740534428 2.062278519879792E-4 42-43 0.0 4.582841155288427E-5 0.0 0.1229117997848356 2.062278519879792E-4 44-45 0.0 4.582841155288427E-5 0.0 0.15595408451446519 2.062278519879792E-4 46-47 0.0 4.582841155288427E-5 0.0 0.1934417251647245 2.062278519879792E-4 48-49 0.0 4.582841155288427E-5 0.0 0.23434358247567372 2.5205626354086346E-4 50-51 0.0 4.582841155288427E-5 0.0 0.2796335101928116 2.5205626354086346E-4 52-53 0.0 4.582841155288427E-5 0.0 0.3257025209063485 2.864275722055267E-4 54-55 0.0 4.582841155288427E-5 0.0 0.37593045996830965 3.3225598375841094E-4 56-57 0.0 4.582841155288427E-5 0.0 0.4306037549509006 3.4371308664663203E-4 58-59 0.0 4.582841155288427E-5 0.0 0.4920711119462066 3.4371308664663203E-4 60-61 0.0 4.582841155288427E-5 0.0 0.5572963986888492 3.4371308664663203E-4 62-63 0.0 4.582841155288427E-5 0.0 0.6256838458286407 3.6662729242307416E-4 64-65 0.0 4.582841155288427E-5 0.0 0.6929255826796101 3.6662729242307416E-4 66-67 0.0 4.582841155288427E-5 0.0 0.7674654940703765 3.6662729242307416E-4 68-69 0.0 4.582841155288427E-5 0.0 0.8553643874288084 3.6662729242307416E-4 70-71 0.0 5.7285514441105334E-5 0.0 0.9498969433595205 3.6662729242307416E-4 72-73 0.0 6.87426173293264E-5 0.0 1.035641901374967 3.895414981995163E-4 74-75 0.0 6.87426173293264E-5 0.0 1.125671815870608 3.895414981995163E-4 76-77 0.0 6.87426173293264E-5 0.0 1.2236644168735629 3.895414981995163E-4 78-79 0.0 6.87426173293264E-5 0.0 1.3325298085174395 3.895414981995163E-4 80-81 0.0 6.87426173293264E-5 0.0 1.4413035433382102 4.124557039759584E-4 82-83 0.0 6.87426173293264E-5 0.0 1.5585440771933765 4.5828411552884267E-4 84-85 0.0 6.87426173293264E-5 0.0 1.6777323185395403 4.5828411552884267E-4 86-87 0.0 6.87426173293264E-5 0.0 1.7999452350481944 4.5828411552884267E-4 88 0.0 1.1457102888221067E-4 0.0 1.8940653352749304 4.5828411552884267E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 7230 0.0 62.645065 2 GTACCTG 7555 0.0 61.04012 1 ACCTGGG 8075 0.0 55.22418 3 TATAACG 710 0.0 45.707726 2 GGTATCA 9930 0.0 44.636444 1 TAACGCA 740 0.0 42.544037 4 CCTGGGG 6545 0.0 38.12242 4 GTATCAA 17265 0.0 36.87043 1 TCAACGC 21270 0.0 29.205158 4 ATCAACG 21395 0.0 29.101086 3 CAACGCA 21590 0.0 28.858356 5 TATCAAC 22370 0.0 28.005098 2 AACGCAG 22630 0.0 27.864016 6 GTACATG 17945 0.0 27.327562 1 ATAACGC 1275 0.0 26.904085 3 TATCACG 545 0.0 26.752481 2 CTGGGGG 4550 0.0 25.920479 5 ACATGGG 18075 0.0 25.60728 3 TACATGG 18875 0.0 25.391369 2 ACGCAGA 25115 0.0 25.013182 7 >>END_MODULE