FastQCFastQC Report
Mon 6 Jun 2016
ERR1211647_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211647_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4234385
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175490.4144403496611669No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147020.34720508409131434No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104680.2472141763207644No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95630.22584153306796617No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50300.11878938736085641No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG59450.053.9806862
GTACCTG62750.051.936551
TATAACG4800.047.0550082
GGTATCA82550.045.7640271
ACCTGGG69800.044.8998453
TAACGCA5500.041.0074964
GTATCAA134250.037.0986751
ATAACGC6850.033.6108863
CCTGGGG65750.031.4442794
TCAACGC162950.029.7872664
CAACGCA164300.029.626215
GTACATG153500.029.5887571
ATCAACG164250.029.5222033
TATCAAC170250.028.7443242
TACATGG156850.028.6200052
AACGCAG171650.028.4644266
CATGGGG105200.027.4244144
ACATGGG157750.027.4026723
ATGGGGG66850.025.302015
GTATAAC12900.025.2271481
Produced by FastQC (version 0.11.5)