##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211647_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4234385 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.16910507665222 31.0 31.0 34.0 28.0 34.0 2 31.158262415911636 31.0 31.0 34.0 28.0 34.0 3 31.285447591562882 31.0 31.0 34.0 28.0 34.0 4 34.87918812295056 37.0 35.0 37.0 32.0 37.0 5 34.9716060301555 37.0 35.0 37.0 32.0 37.0 6 35.08996678384228 37.0 35.0 37.0 32.0 37.0 7 35.039058800746744 37.0 35.0 37.0 32.0 37.0 8 35.03910225451866 37.0 35.0 37.0 32.0 37.0 9 36.53315629070101 39.0 37.0 39.0 32.0 39.0 10-11 36.49854370823626 39.0 37.0 39.0 32.0 39.0 12-13 36.44950305652415 39.0 37.0 39.0 32.0 39.0 14-15 37.5942509951268 40.0 37.0 41.0 32.0 41.0 16-17 37.558798975530095 40.0 37.0 41.0 32.0 41.0 18-19 37.58523150351232 40.0 37.0 41.0 32.0 41.0 20-21 37.549211396696336 40.0 37.0 41.0 31.5 41.0 22-23 37.43820850017181 40.0 37.0 41.0 31.0 41.0 24-25 37.332602018947256 40.0 37.0 41.0 31.0 41.0 26-27 37.17151321856657 40.0 36.5 41.0 30.5 41.0 28-29 37.02462494553518 40.0 36.0 41.0 30.0 41.0 30-31 36.81142786969064 39.5 36.0 41.0 30.0 41.0 32-33 36.59975427364304 39.0 35.5 41.0 30.0 41.0 34-35 36.439353766839815 39.0 35.0 41.0 29.5 41.0 36-37 36.269405120224064 39.0 35.0 40.5 28.5 41.0 38-39 36.104724771129696 39.0 35.0 40.0 28.0 41.0 40-41 35.95222080656341 39.0 35.0 40.0 27.0 41.0 42-43 35.93598302941277 39.0 35.0 40.0 27.0 41.0 44-45 35.86070586401567 39.0 35.0 40.0 27.0 41.0 46-47 35.73876170919744 39.0 35.0 40.0 26.0 41.0 48-49 35.71552480466467 39.0 35.0 40.5 26.0 41.0 50-51 34.744187998965614 38.0 34.0 40.0 25.0 40.5 52-53 34.940081617519425 38.0 33.5 39.5 25.0 40.5 54-55 35.447069763377684 39.0 34.0 40.0 26.0 41.0 56-57 35.393491144522756 38.5 34.0 40.0 26.0 41.0 58-59 35.23370666578499 38.0 34.0 40.0 26.0 41.0 60-61 35.004535841686575 38.0 34.0 40.0 26.0 41.0 62-63 34.724046821439245 37.0 34.0 40.0 25.0 41.0 64-65 34.39432101710166 37.0 33.0 40.0 24.5 41.0 66-67 34.13689768407927 36.0 33.0 39.5 24.0 41.0 68-69 33.810437879408695 36.0 33.0 39.0 23.0 41.0 70-71 33.48327136053996 35.0 32.5 39.0 23.5 40.5 72-73 33.13538683893883 35.0 32.0 38.0 23.0 40.0 74-75 32.76314671433986 35.0 32.0 37.0 22.0 39.5 76-77 32.458361840031074 35.0 32.0 37.0 22.0 39.0 78-79 32.16300454493391 35.0 31.0 36.0 21.5 39.0 80-81 31.894213563480882 35.0 31.0 36.0 21.0 37.5 82-83 31.610332433163258 35.0 31.0 35.5 20.0 37.0 84-85 31.33002242356328 34.5 31.0 35.0 20.0 37.0 86-87 31.03282802579359 34.0 30.5 35.0 19.5 36.0 88-89 30.82276729678572 34.0 30.0 35.0 19.0 36.0 90-91 30.641982720040808 34.0 30.0 35.0 19.0 36.0 92-93 30.495704571029798 34.0 30.0 35.0 18.0 35.0 94-95 30.332515465646132 34.0 29.5 35.0 18.0 35.0 96-97 30.206933710562453 34.0 29.0 35.0 18.0 35.0 98-99 30.065133779757865 34.0 29.0 35.0 17.5 35.0 100 28.781341564359405 33.0 26.0 35.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2925616252464387 1101 2 0.30136034885127216 1101 3 0.1759702101108367 1101 4 0.0960476571621669 1101 5 0.06187608430449387 1101 6 0.1164891145420981 1101 7 0.14876408079529568 1101 8 0.14102941277467806 1101 9 0.10858157745550301 1101 10-11 0.16790027440789856 1101 12-13 0.07837049355591574 1101 14-15 0.1897787553197645 1101 16-17 0.2789857781446159 1101 18-19 0.17294936763207147 1101 20-21 0.12451612036733906 1101 22-23 0.14219927534622911 1101 24-25 0.06683690693648003 1101 26-27 0.14708375039693777 1101 28-29 0.048884010381421206 1101 30-31 0.12753853981079288 1101 32-33 0.07688343859832258 1101 34-35 -0.002410458342971822 1101 36-37 0.07071938957472668 1101 38-39 0.013882317966313451 1101 40-41 -0.020653204644688117 1101 42-43 -0.03462155265172129 1101 44-45 -0.07837989057526329 1101 46-47 -0.12496020985980039 1101 48-49 -0.022819646108523273 1101 50-51 0.0016386142026476591 1101 52-53 -0.003569057991256841 1101 54-55 0.028955946753193018 1101 56-57 0.009021560992827915 1101 58-59 -8.878450777700664E-4 1101 60-61 -0.052421227186108865 1101 62-63 -0.05700897396148008 1101 64-65 -0.14069461642787218 1101 66-67 -0.13961603955202406 1101 68-69 -0.149904650783391 1101 70-71 -0.0011737990232703055 1101 72-73 -0.16631345730797875 1101 74-75 -0.22344937756017202 1101 76-77 -0.24484833139016615 1101 78-79 -0.3066214839824859 1101 80-81 -0.35325887372029285 1101 82-83 -0.44064813404830616 1101 84-85 -0.5253332018057009 1101 86-87 -0.49446068486019 1101 88-89 -0.6712441261357576 1101 90-91 -0.5625480589378427 1101 92-93 -0.5467794917052196 1101 94-95 -0.6681523426775087 1101 96-97 -0.8444676009711074 1101 98-99 -0.805254647023915 1101 100 -0.7943109507205328 1102 1 0.3923478127086959 1102 2 0.4069847121677448 1102 3 0.3428023853153732 1102 4 0.2724039445921562 1102 5 0.2567653774625498 1102 6 0.3510607002618613 1102 7 0.26777836674814637 1102 8 0.2607189132269241 1102 9 0.346686484046522 1102 10-11 0.35546526259137323 1102 12-13 0.4356062890823935 1102 14-15 0.4307426840833912 1102 16-17 0.43001433011819756 1102 18-19 0.4125564298564228 1102 20-21 0.38586380735095815 1102 22-23 0.30408785040875586 1102 24-25 0.3467775307260723 1102 26-27 0.23947293446695994 1102 28-29 0.2067315877383109 1102 30-31 0.36176090915969183 1102 32-33 0.37652420228995 1102 34-35 0.35909943311309434 1102 36-37 0.29359013938388756 1102 38-39 0.259276298138694 1102 40-41 0.1813158208194139 1102 42-43 0.16950340243364082 1102 44-45 0.2725571351948304 1102 46-47 0.15823064463077685 1102 48-49 0.24949857956524824 1102 50-51 0.38524815124382883 1102 52-53 0.37956119203850847 1102 54-55 0.324245552576393 1102 56-57 0.41149925989801517 1102 58-59 0.3885293770400011 1102 60-61 0.2788667280409314 1102 62-63 0.23629959324672 1102 64-65 0.29005622166447864 1102 66-67 0.3212082942433341 1102 68-69 0.3073962204663374 1102 70-71 0.2552115677737916 1102 72-73 0.20594432551687447 1102 74-75 0.23971622776526402 1102 76-77 0.08251205074654422 1102 78-79 0.14061551824613616 1102 80-81 -0.0027708057958903964 1102 82-83 -0.09239995312486471 1102 84-85 -0.10336015220538997 1102 86-87 -0.12913411300942812 1102 88-89 -0.17176917624891175 1102 90-91 -0.23993114754587452 1102 92-93 -0.14267137119231066 1102 94-95 -0.14243760060201538 1102 96-97 -0.23618138199452687 1102 98-99 -0.2696238569410667 1102 100 -0.4500021405557888 1103 1 0.3391689883519007 1103 2 0.3532799646810645 1103 3 0.3540790618298679 1103 4 0.338502430612607 1103 5 0.25272555172637823 1103 6 0.30529989950703396 1103 7 0.3149400149311887 1103 8 0.2473529110310082 1103 9 0.2716806122067936 1103 10-11 0.34877610043363205 1103 12-13 0.3325527709961804 1103 14-15 0.4705806078546715 1103 16-17 0.45123035651443644 1103 18-19 0.3626149330202253 1103 20-21 0.42162179123696575 1103 22-23 0.41584659622146347 1103 24-25 0.3918869810115666 1103 26-27 0.46147437491735843 1103 28-29 0.3805544284819078 1103 30-31 0.43705565130051127 1103 32-33 0.45233572013563617 1103 34-35 0.4450689781067254 1103 36-37 0.35722231819560335 1103 38-39 0.35265235174045273 1103 40-41 0.3427130415626536 1103 42-43 0.21680503312357047 1103 44-45 0.2238064447660264 1103 46-47 0.34670330176672337 1103 48-49 0.3673956780289416 1103 50-51 0.34591516533328104 1103 52-53 0.34997139755155615 1103 54-55 0.43929632213042424 1103 56-57 0.4719142624283492 1103 58-59 0.4149401184845303 1103 60-61 0.3564549179619192 1103 62-63 0.26275494184029213 1103 64-65 0.3839621533122326 1103 66-67 0.23557588566983156 1103 68-69 0.30078793230738654 1103 70-71 0.31314562721536987 1103 72-73 0.3891801625452729 1103 74-75 0.4002529448566605 1103 76-77 0.32272423872182543 1103 78-79 0.22681272650583395 1103 80-81 0.1663276051618645 1103 82-83 0.24715515378835917 1103 84-85 0.20657093808964788 1103 86-87 0.2889459339545297 1103 88-89 0.20204311805172992 1103 90-91 0.13403242902197476 1103 92-93 0.17548790861478025 1103 94-95 0.16537594151501622 1103 96-97 0.13074021085196819 1103 98-99 0.043878092133066815 1103 100 -0.06770224653140033 1104 1 0.29345951862035236 1104 2 0.2764662496256882 1104 3 0.2721971669521821 1104 4 0.2864414804556219 1104 5 0.2482283006405055 1104 6 0.27172664113820133 1104 7 0.25990691365649354 1104 8 0.265972873313558 1104 9 0.240770891894023 1104 10-11 0.32428445713909326 1104 12-13 0.3201417604897685 1104 14-15 0.4416155134791069 1104 16-17 0.4643382159653413 1104 18-19 0.41704491991909265 1104 20-21 0.2680868473479805 1104 22-23 0.3153977123901228 1104 24-25 0.3287454596153907 1104 26-27 0.2755211920507108 1104 28-29 0.3187606875195357 1104 30-31 0.30565245946205266 1104 32-33 0.2897181299292697 1104 34-35 0.2852557991891942 1104 36-37 0.3053514695596178 1104 38-39 0.27837701439641194 1104 40-41 0.21815431861935508 1104 42-43 0.12873863778600736 1104 44-45 0.21164559212122214 1104 46-47 0.2195286136390564 1104 48-49 0.16043308837999604 1104 50-51 0.12900748449368393 1104 52-53 0.2377530992394128 1104 54-55 0.26908091281865154 1104 56-57 0.26252320387035866 1104 58-59 0.29937489360786884 1104 60-61 0.21550957959858152 1104 62-63 0.3262187538379422 1104 64-65 0.24159049721290415 1104 66-67 0.2766756716814598 1104 68-69 0.14775173013902076 1104 70-71 0.22034569043599816 1104 72-73 0.28177310169986924 1104 74-75 0.25965833769351576 1104 76-77 0.21751725358051743 1104 78-79 0.3244086220715232 1104 80-81 0.13246003544467655 1104 82-83 0.14876056045472197 1104 84-85 0.15464567781203442 1104 86-87 0.18650558403964013 1104 88-89 0.139244837774406 1104 90-91 0.15928654088666505 1104 92-93 0.14187402469605814 1104 94-95 0.027252979780541864 1104 96-97 -0.015033543316832265 1104 98-99 -0.08186954553327297 1104 100 -0.11851457841842361 1105 1 0.3114151817478046 1105 2 0.34821371064531803 1105 3 0.3039290545951694 1105 4 0.22343662138087694 1105 5 0.253138402040058 1105 6 0.18826203734341362 1105 7 0.1818088462611911 1105 8 0.19775779415105177 1105 9 0.23078390854424669 1105 10-11 0.24588788923686167 1105 12-13 0.25292698705565186 1105 14-15 0.4441631241481403 1105 16-17 0.4099308079745896 1105 18-19 0.3788681938105185 1105 20-21 0.3590333280518152 1105 22-23 0.37755998447199346 1105 24-25 0.31239195777400397 1105 26-27 0.264919461632374 1105 28-29 0.29008349461958716 1105 30-31 0.22338040133180215 1105 32-33 0.3714688639902448 1105 34-35 0.32500307810949636 1105 36-37 0.30027113548932505 1105 38-39 0.2983083150495318 1105 40-41 0.38146640559207157 1105 42-43 0.26698757793121075 1105 44-45 0.17880562787654952 1105 46-47 0.22542639895299743 1105 48-49 0.3311892946985395 1105 50-51 0.42770238892713053 1105 52-53 0.3016043750027535 1105 54-55 0.35837118498164244 1105 56-57 0.34808793727067666 1105 58-59 0.3204811556889382 1105 60-61 0.3541762262717185 1105 62-63 0.4020765031894271 1105 64-65 0.2581604577702805 1105 66-67 0.24100291928671425 1105 68-69 0.3287447669970902 1105 70-71 0.26487696049469633 1105 72-73 0.30423631666360507 1105 74-75 0.23715321183460958 1105 76-77 0.2924446565743253 1105 78-79 0.2811798261256939 1105 80-81 0.2623599692718521 1105 82-83 0.23950778910352355 1105 84-85 0.2176789629379492 1105 86-87 0.1603906733190179 1105 88-89 0.22642904700049726 1105 90-91 0.17446713099638345 1105 92-93 0.2156473947398112 1105 94-95 0.26047238595979394 1105 96-97 0.19828271856358626 1105 98-99 0.13380440647319602 1105 100 0.2620118612612785 1106 1 0.2639883130629208 1106 2 0.21334236041598942 1106 3 0.17404686463763497 1106 4 0.13393249739169732 1106 5 0.2082578084047313 1106 6 0.22581397531202185 1106 7 0.1583733308832862 1106 8 0.15616131722475757 1106 9 0.21256545012525407 1106 10-11 0.24715736786986753 1106 12-13 0.22955226155093555 1106 14-15 0.36475064180751815 1106 16-17 0.34954858759597585 1106 18-19 0.3081413978225882 1106 20-21 0.2870554524294633 1106 22-23 0.30807005071047655 1106 24-25 0.2914796141576588 1106 26-27 0.29353403376470766 1106 28-29 0.41581294696294435 1106 30-31 0.336810227655711 1106 32-33 0.33684728016680765 1106 34-35 0.29583867908456085 1106 36-37 0.3527070270013013 1106 38-39 0.366693943006311 1106 40-41 0.4420492900490771 1106 42-43 0.3533140066918534 1106 44-45 0.30743438605495754 1106 46-47 0.36848253325230473 1106 48-49 0.4373643749693983 1106 50-51 0.31924416019454327 1106 52-53 0.3622437632025992 1106 54-55 0.41268748551171797 1106 56-57 0.4636844547584005 1106 58-59 0.4134365554709518 1106 60-61 0.46339168879013215 1106 62-63 0.353506701256741 1106 64-65 0.3273603982244353 1106 66-67 0.3841342176768663 1106 68-69 0.4086329052672397 1106 70-71 0.37430782719834355 1106 72-73 0.44468810407404646 1106 74-75 0.4949264228508454 1106 76-77 0.4838648585493317 1106 78-79 0.4686733577277522 1106 80-81 0.35006611384741504 1106 82-83 0.23138133822226337 1106 84-85 0.310162627031616 1106 86-87 0.42363816138528065 1106 88-89 0.4317782317680532 1106 90-91 0.26751436896171654 1106 92-93 0.34605505382476665 1106 94-95 0.33051564869602856 1106 96-97 0.28045923228369674 1106 98-99 0.12197385037810093 1106 100 0.06538782660760134 1107 1 0.14258901700248927 1107 2 0.2565850453965055 1107 3 0.21283875069208236 1107 4 0.24109800708780682 1107 5 0.13685204008131535 1107 6 0.2006701846592449 1107 7 0.1826264271079978 1107 8 0.22060865742283653 1107 9 0.23673156407060247 1107 10-11 0.24608949588516538 1107 12-13 0.20583401380998367 1107 14-15 0.2678501206438355 1107 16-17 0.28956813701748985 1107 18-19 0.3034758935243147 1107 20-21 0.1924178585443812 1107 22-23 0.2489332048627091 1107 24-25 0.25021731827170157 1107 26-27 0.2668562550947158 1107 28-29 0.31924675750957476 1107 30-31 0.2875546612782003 1107 32-33 0.3292490284900609 1107 34-35 0.280538254526725 1107 36-37 0.27281146434162196 1107 38-39 0.20860033444569126 1107 40-41 0.3124167511093461 1107 42-43 0.35365031760888144 1107 44-45 0.314609884318962 1107 46-47 0.321538166233978 1107 48-49 0.27211955840895996 1107 50-51 0.27007714403939076 1107 52-53 0.33733010031198774 1107 54-55 0.2630645732277941 1107 56-57 0.25651898618866653 1107 58-59 0.25121054322495695 1107 60-61 0.2987982625735768 1107 62-63 0.28080167369127196 1107 64-65 0.2114595954479057 1107 66-67 0.23062671381022426 1107 68-69 0.3016614092368073 1107 70-71 0.20173184905125652 1107 72-73 0.3030876620066607 1107 74-75 0.308843130018424 1107 76-77 0.35761761131741565 1107 78-79 0.28047280208988923 1107 80-81 0.289222394991004 1107 82-83 0.3363162562507185 1107 84-85 0.37266397006473895 1107 86-87 0.26119985994150596 1107 88-89 0.3912117170050742 1107 90-91 0.38282786142464076 1107 92-93 0.2805916094642775 1107 94-95 0.3044509100268833 1107 96-97 0.34690539133233855 1107 98-99 0.2704500308566651 1107 100 0.2887595538093777 1108 1 0.3423914713869003 1108 2 0.41325552448035197 1108 3 0.35193589049734086 1108 4 0.28917054866789016 1108 5 0.23615304850257246 1108 6 0.23687843396507446 1108 7 0.25156152507674534 1108 8 0.19592462879174377 1108 9 0.28666035936214485 1108 10-11 0.23398550634862403 1108 12-13 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94-95 0.3987913715896383 2316 96-97 0.37111436217336546 2316 98-99 0.36678888161958056 2316 100 0.17054492020985634 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 1.0 1 21.0 2 47.0 3 38.0 4 47.0 5 62.0 6 152.0 7 1859.0 8 3486.0 9 4053.0 10 5399.0 11 8041.0 12 10740.0 13 13358.0 14 14486.0 15 15597.0 16 17603.0 17 20475.0 18 24610.0 19 28588.0 20 32155.0 21 37116.0 22 42031.0 23 45446.0 24 45640.0 25 46204.0 26 49095.0 27 54337.0 28 62798.0 29 74309.0 30 89462.0 31 109689.0 32 138377.0 33 182071.0 34 253665.0 35 369221.0 36 559698.0 37 863355.0 38 828031.0 39 182840.0 40 182.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.926886505442305 17.264088270125068 11.602310925662952 24.206714298769676 2 16.322212592509892 19.792007675175086 39.926427315751326 23.959352416563696 3 18.588374860126557 26.204813229234652 29.859062370151264 25.34774954048753 4 12.339085550949056 15.700648453279472 37.2674013441527 34.69286465161877 5 12.693259034186678 37.05778591566329 34.737213063951685 15.511741986198349 6 30.940163754202903 35.802367962575865 18.890846197210244 14.366622086010988 7 27.602558637408674 32.151614735427586 22.54321567868011 17.702610948483628 8 26.8937300613381 34.505398376472165 20.43724062617199 18.16363093601774 9 27.407446419580012 14.996613288567653 20.03818434479651 37.557755947055824 10-11 25.180816109827365 25.294393000130366 28.983764338226266 20.541026551816003 12-13 25.484366549446293 23.351089582659256 28.974798013315418 22.189745854579034 14-15 22.848303472884687 24.60484514190336 26.657491675076812 25.88935971013514 16-17 22.4779651933256 27.27034594684861 27.24576933247152 23.00591952735427 18-19 22.22124988455951 26.489784667754584 29.182164036276774 22.106801411409137 20-21 23.005703792970362 25.842471574334724 29.124904279356418 22.026920353338493 22-23 22.74264485979984 25.578255686883704 29.093240466582664 22.585858986733793 24-25 22.345600811691302 25.747191507471918 29.168125056387133 22.73908262444965 26-27 22.302184102121203 25.830790573896188 29.008408259951835 22.85861706403077 28-29 22.38189494412892 26.107736152235056 29.03411133773574 22.476257565900283 30-31 22.867849567513687 25.919228280022487 29.10285662996084 22.11006552250299 32-33 22.514094029644678 26.032791524342546 29.009565857943237 22.443548588069543 34-35 22.708273100565574 26.16532690094615 28.868727052496517 22.257672945991757 36-37 22.691211943724067 26.14168384880943 28.833271384312003 22.333832823154502 38-39 22.701989852596295 26.383639422858295 28.558037515037732 22.356333209507685 40-41 22.711942445635273 26.11998115287562 28.749955852890846 22.41812054859826 42-43 22.479661073407716 26.275114915496328 28.62780845157201 22.61741555952395 44-45 22.340515892503436 26.48166198714858 28.57442075827685 22.60340136207113 46-47 22.356323569789996 26.267374058557643 28.432725436879448 22.943576934772917 48-49 22.46436184153724 26.421413096256245 28.367599722059396 22.746625340147123 50-51 22.36243929360736 26.761514358543316 28.228837220947828 22.6472091269015 52-53 22.534659330878622 26.78966914310928 27.94229917051928 22.73337235549282 54-55 22.356971637625264 26.669731115843735 28.268787782012783 22.704509464518214 56-57 22.461821004806488 26.70409953167981 27.970777046854927 22.863302416658772 58-59 22.46726250718308 26.8098118357347 28.101845411342293 22.62108024573993 60-61 22.720933498684097 26.824420806745252 28.09958560333322 22.35506009123743 62-63 22.649871424121805 26.86852565735754 28.028950863119697 22.45265205540096 64-65 22.538695529504686 26.602728495269712 28.038201815398757 22.820374159826848 66-67 22.420270873019728 26.688563339924066 28.049978548534693 22.841187238521517 68-69 22.317723801511413 26.709145525564264 28.116220324867836 22.856910348056484 70-71 22.31321845698432 26.60765887206793 28.083609611271598 22.995513059676153 72-73 22.263808925438884 27.03780259671596 27.710115715533245 22.988272762311908 74-75 22.405046648756322 27.004870002347175 27.721874398662145 22.868208950234354 76-77 22.38209299351278 26.82028715330317 27.80575211201008 22.991867741173973 78-79 22.311058604181426 26.903165929311445 27.87845170637221 22.907323760134922 80-81 22.476019650529704 26.98136818194809 27.551886860414776 22.990725307107432 82-83 22.58487641015817 27.04610085463988 27.631234263828873 22.737788471373072 84-85 22.525799480998973 27.22165576301583 27.437709366511232 22.814835389473966 86-87 22.462777067704824 27.132898613588296 27.554612273201958 22.849712045504926 88-89 22.28355171754411 26.955445085560964 27.748686523470372 23.01231667342455 90-91 22.691180631961252 26.988017802284293 27.579285688303184 22.741515877451274 92-93 22.538119446542424 27.038744510392238 27.580190938257154 22.84294510480818 94-95 22.51309030372717 26.93129220335652 27.51224233926704 23.043375153649272 96-97 22.49878031915189 26.9419734253067 27.524385054690477 23.034861200850937 98-99 22.53684457198688 26.92808587759391 27.354596583000802 23.18047296741841 100 22.64567608448992 26.905111251329707 27.451451935745023 22.997760728435345 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2503.0 1 1900.5 2 4465.0 3 6392.0 4 3561.0 5 2239.5 6 3267.0 7 5918.5 8 9078.0 9 9927.5 10 7607.5 11 5318.0 12 5102.0 13 5279.5 14 5528.0 15 5833.5 16 6078.0 17 6471.0 18 6896.0 19 7320.0 20 7792.0 21 8641.0 22 9983.0 23 11910.5 24 14767.5 25 18114.0 26 21950.0 27 26754.0 28 32043.5 29 37830.0 30 44357.0 31 50857.5 32 57687.5 33 64744.5 34 71272.5 35 77682.5 36 86275.0 37 94981.5 38 102172.0 39 108682.0 40 113941.0 41 119994.0 42 126260.5 43 131746.0 44 136932.0 45 142877.5 46 148874.0 47 154536.0 48 161481.5 49 167570.0 50 170677.0 51 168243.0 52 162033.5 53 156999.0 54 151624.5 55 141964.0 56 130570.5 57 119416.5 58 107412.0 59 94646.0 60 78504.5 61 63056.5 62 51578.0 63 40612.0 64 30451.5 65 23084.0 66 17655.5 67 13594.0 68 10557.5 69 8123.0 70 6466.5 71 5145.0 72 4041.0 73 3349.0 74 2764.5 75 2132.0 76 1665.0 77 1258.5 78 915.0 79 732.5 80 594.0 81 481.5 82 393.0 83 317.5 84 269.0 85 224.0 86 178.5 87 131.0 88 97.0 89 70.0 90 55.0 91 44.0 92 29.5 93 18.5 94 13.5 95 11.5 96 7.5 97 7.5 98 8.0 99 6.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2508746842811884 2 0.15891327784318146 3 0.025836101346476525 4 0.029165982781442876 5 0.016271548288594447 6 0.0026450122036612163 7 0.0023616180389832288 8 0.003518810878085011 9 0.004463458093678303 10-11 0.00588042891706824 12-13 0.004605155176017296 14-15 0.009729866320610904 16-17 0.016188891657230035 18-19 0.014216940594679038 20-21 0.016791104257170758 22-23 0.02888258861676489 24-25 0.006211055442525892 26-27 0.014913617916179091 28-29 0.02944937694612087 30-31 0.01712173078262841 32-33 0.022281866197806765 34-35 0.019105489935374323 36-37 0.02516304020536631 38-39 0.030287751349959913 40-41 0.03255490466738381 42-43 0.035235341141629774 44-45 0.04566188478374073 46-47 0.012339454253687373 48-49 0.026131303601349427 50-51 0.018668590598162425 52-53 0.021089249088120234 54-55 0.020994784366560906 56-57 0.007462713003187004 58-59 0.011288534226339833 60-61 0.03220066196153633 62-63 0.03026413516957008 64-65 0.015574870967094395 66-67 0.00964720968924649 68-69 0.02511580784458664 70-71 0.020699582111688004 72-73 0.03451504763973989 74-75 0.03862426302757071 76-77 0.010497392183280453 78-79 0.010544624544060117 80-81 0.014311405316238368 82-83 0.021396259433188056 84-85 0.011382998947899164 86-87 0.01108779669302626 88-89 0.02499772694263748 90-91 0.018208075080560694 92-93 0.019306227468687896 94-95 0.01693280133950975 96-97 0.01642505346112836 98-99 0.028268567926629247 100 0.030984428671459965 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4234385.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.46205908253392 #Duplication Level Percentage of deduplicated Percentage of total 1 75.53048203721514 47.17789431540799 2 15.150348892881496 18.92643975336333 3 4.634172552936001 8.683798794004367 4 1.8464561613447366 4.613338153728949 5 0.8699276246010113 2.716873534267837 6 0.5038783060594548 1.8883965914095666 7 0.3268889922996578 1.4292711685315838 8 0.21840767985064177 1.0913754722327953 9 0.16154372452789695 0.9081318299286675 >10 0.7138057568260486 7.52828400864258 >50 0.030252519018156634 1.281564869088938 >100 0.012653212257096855 1.3754459198475628 >500 5.676192897269476E-4 0.2429826687056216 >1k 3.784128572746847E-4 0.44288791158467566 >5k 9.460321431867114E-5 0.4311583251337673 >10k+ 1.4190482147800673E-4 1.2621566841217984 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17549 0.4144403496611669 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14702 0.34720508409131434 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10468 0.2472141763207644 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9563 0.22584153306796617 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5030 0.11878938736085641 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.361618038983229E-5 2 0.0 0.0 0.0 0.0 2.361618038983229E-5 3 0.0 0.0 0.0 0.0 2.361618038983229E-5 4 0.0 0.0 0.0 0.0 2.361618038983229E-5 5 0.0 0.0 0.0 0.0 2.361618038983229E-5 6 0.0 0.0 0.0 0.0 2.361618038983229E-5 7 0.0 0.0 0.0 0.0 2.361618038983229E-5 8 0.0 0.0 0.0 0.0 2.361618038983229E-5 9 0.0 0.0 0.0 0.0 2.361618038983229E-5 10-11 0.0 0.0 0.0 0.0 2.361618038983229E-5 12-13 0.0 2.361618038983229E-5 0.0 0.0 2.361618038983229E-5 14-15 0.0 2.361618038983229E-5 0.0 0.0 4.723236077966458E-5 16-17 2.361618038983229E-5 2.361618038983229E-5 0.0 0.0 7.084854116949687E-5 18-19 2.361618038983229E-5 2.361618038983229E-5 0.0 9.446472155932915E-5 7.084854116949687E-5 20-21 2.361618038983229E-5 2.361618038983229E-5 0.0 1.4169708233899373E-4 7.084854116949687E-5 22-23 2.361618038983229E-5 2.361618038983229E-5 0.0 1.7712135292374218E-4 7.084854116949687E-5 24-25 2.361618038983229E-5 2.361618038983229E-5 0.0 3.306265254576521E-4 7.084854116949687E-5 26-27 2.361618038983229E-5 2.361618038983229E-5 0.0 6.022125999407234E-4 7.084854116949687E-5 28-29 2.361618038983229E-5 2.361618038983229E-5 0.0 0.0015940921763136794 7.084854116949687E-5 30-31 2.361618038983229E-5 2.361618038983229E-5 0.0 0.00541991339946651 7.084854116949687E-5 32-33 2.361618038983229E-5 2.361618038983229E-5 0.0 0.013768233167272225 7.084854116949687E-5 34-35 2.361618038983229E-5 2.361618038983229E-5 0.0 0.025694404264137532 7.084854116949687E-5 36-37 2.361618038983229E-5 2.361618038983229E-5 0.0 0.04345377191729141 7.084854116949687E-5 38-39 2.361618038983229E-5 2.361618038983229E-5 0.0 0.07466255430245478 8.265663136441301E-5 40-41 2.361618038983229E-5 2.361618038983229E-5 0.0 0.11506983894945783 9.446472155932915E-5 42-43 2.361618038983229E-5 7.084854116949687E-5 0.0 0.15075388751849442 9.446472155932915E-5 44-45 2.361618038983229E-5 7.084854116949687E-5 0.0 0.1917043443144636 9.446472155932915E-5 46-47 2.361618038983229E-5 7.084854116949687E-5 0.0 0.23855884620789086 1.1808090194916144E-4 48-49 2.361618038983229E-5 7.084854116949687E-5 0.0 0.28938086640680993 1.889294431186583E-4 50-51 2.361618038983229E-5 7.084854116949687E-5 0.0 0.3457999213581193 1.889294431186583E-4 52-53 2.361618038983229E-5 7.084854116949687E-5 0.0 0.40349425005047956 2.2435371370340676E-4 54-55 2.361618038983229E-5 7.084854116949687E-5 0.0 0.4687103321970014 2.597779842881552E-4 56-57 2.361618038983229E-5 7.084854116949687E-5 0.0 0.5373035281392693 2.7158607448307133E-4 58-59 2.361618038983229E-5 7.084854116949687E-5 0.0 0.6148236402688938 2.8339416467798746E-4 60-61 2.361618038983229E-5 7.084854116949687E-5 0.0 0.6955555529315356 2.8339416467798746E-4 62-63 2.361618038983229E-5 7.084854116949687E-5 0.0 0.7823213996837793 3.070103450678198E-4 64-65 2.361618038983229E-5 7.084854116949687E-5 0.0 0.8650134553187772 3.070103450678198E-4 66-67 2.361618038983229E-5 7.084854116949687E-5 0.0 0.95684497276464 3.070103450678198E-4 68-69 2.361618038983229E-5 7.084854116949687E-5 0.0 1.0649480384990972 3.070103450678198E-4 70-71 2.361618038983229E-5 7.084854116949687E-5 0.0 1.1821079094130553 3.3062652545765204E-4 72-73 2.361618038983229E-5 7.084854116949687E-5 0.0 1.2839172630736222 4.0147506662714894E-4 74-75 2.361618038983229E-5 7.084854116949687E-5 0.0 1.386730304400757 4.0147506662714894E-4 76-77 2.361618038983229E-5 7.084854116949687E-5 0.0 1.5087669165652153 4.0147506662714894E-4 78-79 2.361618038983229E-5 7.084854116949687E-5 0.0 1.6464728644183277 4.0147506662714894E-4 80-81 2.361618038983229E-5 7.084854116949687E-5 0.0 1.7799633240718546 4.0147506662714894E-4 82-83 2.361618038983229E-5 7.084854116949687E-5 0.0 1.915484775238907 4.0147506662714894E-4 84-85 4.723236077966458E-5 7.084854116949687E-5 0.0 2.0517265199078496 4.0147506662714894E-4 86-87 4.723236077966458E-5 7.084854116949687E-5 0.0 2.1991623340815725 4.0147506662714894E-4 88 4.723236077966458E-5 7.084854116949687E-5 0.0 2.3161805079131916 4.487074274068135E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 5945 0.0 53.980686 2 GTACCTG 6275 0.0 51.93655 1 TATAACG 480 0.0 47.055008 2 GGTATCA 8255 0.0 45.764027 1 ACCTGGG 6980 0.0 44.899845 3 TAACGCA 550 0.0 41.007496 4 GTATCAA 13425 0.0 37.098675 1 ATAACGC 685 0.0 33.610886 3 CCTGGGG 6575 0.0 31.444279 4 TCAACGC 16295 0.0 29.787266 4 CAACGCA 16430 0.0 29.62621 5 GTACATG 15350 0.0 29.588757 1 ATCAACG 16425 0.0 29.522203 3 TATCAAC 17025 0.0 28.744324 2 TACATGG 15685 0.0 28.620005 2 AACGCAG 17165 0.0 28.464426 6 CATGGGG 10520 0.0 27.424414 4 ACATGGG 15775 0.0 27.402672 3 ATGGGGG 6685 0.0 25.30201 5 GTATAAC 1290 0.0 25.227148 1 >>END_MODULE