FastQCFastQC Report
Mon 6 Jun 2016
ERR1211647_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211647_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4234385
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201780.47652728790603593No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166310.39276069606330083No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127030.2999963394920396No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98820.2333750946123227No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50880.12015912582346669No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG64950.055.3236852
GTACCTG69050.052.8002551
ACCTGGG74300.047.3729863
TATAACG5100.045.1879652
GGTATCA92300.042.7704661
CCTGGGG67550.036.6608474
GTATCAA151400.035.8878021
TAACGCA6350.034.780944
ATAACGC6900.030.6479323
TCAACGC193000.027.8782424
ATCAACG194150.027.762833
CAACGCA195100.027.6979455
TATCAAC198450.027.3259372
GTACATG162750.027.1253341
AACGCAG203800.026.7470656
CTGGGGG43950.026.4085775
TACATGG166000.026.0661352
CATGGGG102750.025.7022824
ACATGGG163600.025.2776763
ACGCAGA229500.023.6699587
Produced by FastQC (version 0.11.5)