FastQCFastQC Report
Mon 6 Jun 2016
ERR1211646_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211646_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6041069
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178930.2961893002711937No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150870.24974056743930587No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95600.15825013751705203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87000.044.5576441
GTATCAA148600.037.5753061
TACCTGG55000.033.272552
GTACCTG59500.031.8624171
TCAACGC173700.031.2733944
ATCAACG175050.030.842773
CAACGCA176150.030.7555925
AACGCAG183700.029.615526
TATCAAC190150.028.7728272
GTACATG174650.028.3524321
TACATGG176250.026.9849222
ACGCAGA207450.026.0664447
ACATGGG176900.025.9514833
CGCAGAG212000.025.2860228
ACCTGGG70750.024.7729553
CATGGGG106400.023.716454
TATAACG5850.021.712362
GCAGAGT240350.021.5800329
TAACGCA5550.019.4738524
AGAGTAC221400.019.33328210-11
Produced by FastQC (version 0.11.5)