FastQCFastQC Report
Mon 6 Jun 2016
ERR1211646_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211646_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6041069
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202060.33447722580225453No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176610.29234892036492216No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118590.19630631598480333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96950.046.9669271
GTATCAA163800.036.403191
TACCTGG56600.033.0681722
GTACCTG58800.032.226381
TCAACGC198050.029.1833934
ATCAACG199950.028.93033
CAACGCA201550.028.6996885
AACGCAG210500.027.6808036
TATCAAC211700.027.5228862
GTACATG192250.027.2156941
TATAACG6350.026.6607482
ACCTGGG68500.026.2738973
TAACGCA7200.026.1055584
TACATGG197250.025.724562
ACATGGG194350.024.952353
CATGGGG115050.024.138284
ACGCAGA239950.024.0284467
CGCAGAG244800.023.4372228
ATAACGC8350.023.073513
GCAGAGT276550.020.3894679
Produced by FastQC (version 0.11.5)