FastQCFastQC Report
Mon 6 Jun 2016
ERR1211645_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211645_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4872970
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164720.3380279377874274No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138650.28452873709462606No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86230.1769557374660628No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82400.050.083231
GTATCAA143650.038.1843641
TCAACGC174050.031.0196044
CAACGCA175300.030.8220565
ATCAACG176350.030.668333
AACGCAG180500.030.0865786
TATCAAC181800.029.894072
GTACATG177900.026.7501771
TACATGG176700.026.4429322
ACGCAGA204250.026.2891397
TACCTGG42000.025.3195552
CGCAGAG210900.025.2377098
GTACCTG43950.025.1112561
ACATGGG179000.025.0954553
CATGGGG112950.023.587784
GCAGAGT232000.022.0716679
GAGTACT136100.020.86686112-13
TAACGCA4800.020.5574054
ACCTGGG50900.020.4016253
ATGGGGG61800.019.994615
Produced by FastQC (version 0.11.5)