FastQCFastQC Report
Mon 6 Jun 2016
ERR1211645_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211645_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4872970
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189090.3880385062908247No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162800.33408783554998284No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105060.2155974693051671No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91750.049.28981
GTATCAA159000.039.1787151
TCAACGC200200.030.5617394
ATCAACG201550.030.380353
CAACGCA203150.030.047625
TATCAAC206700.029.8774322
AACGCAG210650.029.0685276
ACGCAGA237650.025.607267
GTACATG178950.025.350581
CGCAGAG245450.024.6786298
GTACCTG41400.024.3757461
TACCTGG40800.023.978512
TATAACG5150.023.7457082
TACATGG185450.023.7393052
ATAACGC5200.023.4963933
ACATGGG186450.022.859993
GCAGAGT268950.022.1204179
CATGGGG117350.021.4641084
GAGTACT165600.020.27525712-13
AGAGTAC246800.020.15472210-11
Produced by FastQC (version 0.11.5)