FastQCFastQC Report
Mon 6 Jun 2016
ERR1211644_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211644_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5100906
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145660.2855571147556924No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131170.2571503964197733No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77440.1518161675592532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80700.046.6979561
GTATCAA134400.034.53191
TCAACGC159000.028.3985044
ATCAACG162250.027.9165333
CAACGCA162000.027.8991455
GTACATG175300.027.4437141
AACGCAG168250.027.0547926
GTACCTG44300.026.2977071
TATCAAC172950.026.2513242
TACATGG180350.026.0872522
TACCTGG44550.025.3459172
ACATGGG182400.025.0386333
ACGCAGA192900.023.5488537
CATGGGG103100.023.0600914
CGCAGAG196850.022.8867578
GCAGAGT220100.019.9568799
ACCTGGG54600.019.448453
GAGTACT130900.019.2924212-13
TAACGCA4150.019.2473094
AGAGTAC200550.018.85845810-11
Produced by FastQC (version 0.11.5)