FastQCFastQC Report
Mon 6 Jun 2016
ERR1211644_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211644_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5100906
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177730.34842829881593584No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161650.3169044871636529No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103310.20253264812172583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94000.046.332351
GTATCAA160600.036.128731
TCAACGC196500.028.9117054
ATCAACG196850.028.8608653
CAACGCA197850.028.7378985
AACGCAG205150.027.7395616
TATCAAC206950.027.5634292
GTACCTG43700.026.7491781
TACCTGG43050.025.7796342
ACGCAGA234000.024.2191247
GTACATG189500.023.3807721
CGCAGAG241850.023.3358658
TACATGG190100.022.634812
ACATGGG191450.021.8941443
GCAGAGT264900.020.968069
CATGGGG106100.020.9486334
GAGTACT157400.020.48037112-13
GTATAGG21900.020.4465351
TAACGCA4650.020.2109624
ACCTGGG54650.019.9487763
Produced by FastQC (version 0.11.5)