FastQCFastQC Report
Mon 6 Jun 2016
ERR1211643_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211643_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126115
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACCTGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG2190.1736510327875352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG4500.080.517022
GTACCTG4800.076.6171651
ACCTGGG5200.067.7876053
GTACACG301.3452017E-462.8653641
CCTGGGG3100.062.16054
ATAACGC250.00522552456.399293
CTGGGGG2400.045.0232355
GTACCCG459.948883E-441.9102441
GTATCAT608.617059E-539.290851
CCTGGGA2700.038.295814
GTTAGAG500.001696390737.5995253
TAGCTAA250.001699613937.59952560-61
AATACTG651.4097567E-436.1390535
GTTATAC550.00266614534.29021
GTACATG5050.031.7438981
GGGTTAG600.004083262331.4326821
GTATATG600.00414732831.3329353
ATCATGC600.00414732831.3329353
CTAATAT600.00414732831.3329353
TGGGGGG2000.030.53756
Produced by FastQC (version 0.11.5)