##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211643_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 126115 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.465020021409032 31.0 31.0 34.0 26.0 34.0 2 30.40989573008762 31.0 31.0 34.0 26.0 34.0 3 30.549395393093604 31.0 31.0 34.0 26.0 34.0 4 34.049264560123696 37.0 35.0 37.0 28.0 37.0 5 34.098418110454745 37.0 35.0 37.0 28.0 37.0 6 34.235475558022436 37.0 35.0 37.0 29.0 37.0 7 34.170106648693654 37.0 35.0 37.0 29.0 37.0 8 34.176830670419854 37.0 35.0 37.0 29.0 37.0 9 35.66389406494073 39.0 35.0 39.0 28.0 39.0 10-11 35.53291440352059 39.0 35.0 39.0 28.0 39.0 12-13 35.493497997859095 39.0 35.0 39.0 27.5 39.0 14-15 36.62640843674424 40.0 36.0 41.0 27.0 41.0 16-17 36.57778218292828 40.0 36.0 41.0 27.0 41.0 18-19 36.555794314712756 40.0 36.0 41.0 27.0 41.0 20-21 36.498667882488206 40.0 36.0 41.0 27.0 41.0 22-23 36.381290885303095 40.0 36.0 41.0 27.0 41.0 24-25 36.29218570352457 40.0 36.0 41.0 27.0 41.0 26-27 36.13168536653055 40.0 36.0 41.0 26.5 41.0 28-29 35.98280141141022 39.0 35.5 41.0 26.0 41.0 30-31 35.80897989929826 39.0 35.0 41.0 25.0 41.0 32-33 35.60781429647544 39.0 35.0 41.0 25.0 41.0 34-35 35.47900725528288 39.0 35.0 41.0 24.0 41.0 36-37 35.32085398247631 39.0 35.0 40.0 24.0 41.0 38-39 35.169932204733776 39.0 34.0 40.0 23.5 41.0 40-41 35.034254450303294 39.0 34.0 40.0 23.0 41.0 42-43 35.05804226301392 39.0 34.0 40.0 23.0 41.0 44-45 35.023101930777464 39.0 34.0 40.0 23.0 41.0 46-47 34.95374856281965 39.0 34.0 40.0 22.0 41.0 48-49 35.013138801887166 39.0 34.0 41.0 22.0 41.0 50-51 34.13049994053047 38.0 33.0 40.0 21.0 40.5 52-53 34.35631368195695 38.0 33.5 39.5 22.0 40.5 54-55 34.863220076913926 39.0 34.0 40.0 22.0 41.0 56-57 34.82113943622884 39.0 34.0 41.0 22.0 41.0 58-59 34.672679697101856 38.0 34.0 40.0 22.0 41.0 60-61 34.46580105459303 38.0 33.0 40.0 21.5 41.0 62-63 34.20122110771915 38.0 33.0 40.0 21.0 41.0 64-65 33.894251278594936 37.0 33.0 40.0 20.0 41.0 66-67 33.64972049320065 37.0 32.5 40.0 20.0 41.0 68-69 33.33892082623002 36.0 32.0 39.0 20.0 41.0 70-71 33.02153986440946 36.0 32.0 39.0 19.0 41.0 72-73 32.681382071918485 35.0 31.5 38.5 18.5 40.0 74-75 32.31537485628196 35.0 31.0 37.5 18.0 40.0 76-77 32.01473258533878 35.0 31.0 37.0 18.0 39.0 78-79 31.71204852713793 35.0 31.0 36.5 18.0 39.0 80-81 31.428370931292868 35.0 31.0 36.0 18.0 38.5 82-83 31.145284066130117 35.0 30.5 36.0 17.5 37.0 84-85 30.820473377472943 34.5 30.0 35.0 17.0 37.0 86-87 30.5386353724775 34.0 30.0 35.0 15.5 36.5 88-89 30.309277246957144 34.0 29.0 35.0 15.0 36.0 90-91 30.141973595527894 34.0 29.0 35.0 15.0 36.0 92-93 29.972160329857672 34.0 29.0 35.0 13.0 35.5 94-95 29.825445030329462 34.0 29.0 35.0 12.0 35.0 96-97 29.704638623478573 34.0 29.0 35.0 11.0 35.0 98-99 29.566613804860644 34.0 29.0 35.0 10.5 35.0 100 28.309011616381873 33.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.741451824009328 1101 2 0.41370963143515027 1101 3 0.3184753317412543 1101 4 0.8286526133244507 1101 5 0.30277357325370957 1101 6 0.33901526300987683 1101 7 0.7205767184231888 1101 8 0.8665886845966 1101 9 0.8789510732938624 1101 10-11 0.8275814223138909 1101 12-13 1.001130528466902 1101 14-15 0.643769097337568 1101 16-17 1.0182053020506885 1101 18-19 0.8680483918166217 1101 20-21 0.8879507462811986 1101 22-23 0.31322961909788916 1101 24-25 0.7827382901074671 1101 26-27 0.9495074956598728 1101 28-29 0.8019205743159574 1101 30-31 1.1479430785335936 1101 32-33 0.7837177465676888 1101 34-35 0.8318332178290646 1101 36-37 0.684339689894891 1101 38-39 0.9153003165255456 1101 40-41 0.8467552251118917 1101 42-43 0.8337487928603409 1101 44-45 0.811002946527374 1101 46-47 0.7118788810629155 1101 48-49 1.2825712916210819 1101 50-51 1.223765857741789 1101 52-53 0.6584659056066542 1101 54-55 0.7368358997089501 1101 56-57 1.2470319392496094 1101 58-59 0.994636977845829 1101 60-61 0.8329301062724781 1101 62-63 1.1036980049103207 1101 64-65 0.9725595622599883 1101 66-67 0.7667371987152478 1101 68-69 0.6672766198928173 1101 70-71 0.5364904233526246 1101 72-73 0.33083783098943087 1101 74-75 0.15072571031686977 1101 76-77 0.06639969211941121 1101 78-79 -0.04090436202288217 1101 80-81 -0.1534841051500173 1101 82-83 -0.8876023271000726 1101 84-85 -0.2633769151913228 1101 86-87 -0.15140692353275753 1101 88-89 -0.3006046591736933 1101 90-91 -0.457229790297621 1101 92-93 -0.5939186981083893 1101 94-95 -0.5035175113531238 1101 96-97 0.5673689816141696 1101 98-99 0.10582246329339284 1101 100 -0.09158620797108341 1102 1 1.0998286821853043 1102 2 1.1233463185728425 1102 3 1.2078744019358254 1102 4 1.1688351306865599 1102 5 1.0268165050105793 1102 6 0.8039753732090347 1102 7 0.9478624901042991 1102 8 0.6110643776460165 1102 9 1.1871643545887025 1102 10-11 0.6490243425916802 1102 12-13 0.6362392927440084 1102 14-15 0.8207765013437722 1102 16-17 1.0168852079222361 1102 18-19 0.8129201131045676 1102 20-21 1.3258202291486683 1102 22-23 1.438294188919393 1102 24-25 1.1692101950606926 1102 26-27 0.9146397920497122 1102 28-29 1.1963417417383297 1102 30-31 1.2840849599547042 1102 32-33 1.3533957583911373 1102 34-35 1.2411197930457334 1102 36-37 1.4478419570130612 1102 38-39 1.459609562923987 1102 40-41 0.8971196858821386 1102 42-43 0.7484879307814793 1102 44-45 0.6855940043245425 1102 46-47 0.7353822960890355 1102 48-49 0.9549583661777064 1102 50-51 0.9140315984293892 1102 52-53 0.6427395668590279 1102 54-55 0.8704523836669367 1102 56-57 0.8439727645954207 1102 58-59 1.044972740917629 1102 60-61 0.5277013856158703 1102 62-63 0.7712351684298042 1102 64-65 0.7518455635226928 1102 66-67 0.7071033813474088 1102 68-69 0.7422350082300753 1102 70-71 1.3595117170448603 1102 72-73 0.9356992211059421 1102 74-75 1.0039943781697076 1102 76-77 1.4440327059516989 1102 78-79 0.7907549389011841 1102 80-81 0.6691641184624508 1102 82-83 0.8294957616332148 1102 84-85 0.524030534731196 1102 86-87 0.4847923532852434 1102 88-89 0.7965083523627818 1102 90-91 0.9118513093981839 1102 92-93 0.9301299731881727 1102 94-95 1.0441206680648882 1102 96-97 0.8564695957449153 1102 98-99 1.0474800423555486 1102 100 1.1975144061596659 1103 1 -0.24004642932228037 1103 2 -0.3446032162812429 1103 3 0.14995417764628627 1103 4 -0.8119218465021802 1103 5 -0.3110833293210149 1103 6 -0.15639164603398825 1103 7 0.23338561996284568 1103 8 -0.3461167106679852 1103 9 -0.17571317822638832 1103 10-11 -0.4235830232806208 1103 12-13 -0.7175264776140864 1103 14-15 0.012219090868427429 1103 16-17 0.455842771322267 1103 18-19 -0.31069400694131133 1103 20-21 -0.30305067693008425 1103 22-23 -0.9759086911621679 1103 24-25 -0.8855240982998325 1103 26-27 -0.19122869272154475 1103 28-29 -0.8038175548081199 1103 30-31 -0.6757404510303857 1103 32-33 -0.8411690434439549 1103 34-35 -0.8489146150082973 1103 36-37 -0.924115478949723 1103 38-39 -1.0194907301816585 1103 40-41 -1.1980759302769073 1103 42-43 -1.1962680269093582 1103 44-45 -1.1600476421645638 1103 46-47 -1.2759914773275511 1103 48-49 -0.9782645215500949 1103 50-51 -0.437121708566913 1103 52-53 -0.55682714653463 1103 54-55 -0.7420746967459522 1103 56-57 -0.49284385322030744 1103 58-59 -0.8614246083878356 1103 60-61 -0.12155947840859938 1103 62-63 -0.7508433329082891 1103 64-65 -0.4761077944485095 1103 66-67 -0.2836574189584411 1103 68-69 -0.8140922505949533 1103 70-71 -0.6436104952655697 1103 72-73 -0.4374051499912994 1103 74-75 -0.40351804696091165 1103 76-77 -0.9269370914230954 1103 78-79 -1.108442206504705 1103 80-81 -1.112631472209145 1103 82-83 -1.1624438331163738 1103 84-85 -0.9262052241575489 1103 86-87 -0.8441550151358115 1103 88-89 -1.4612334597947303 1103 90-91 -0.9302858130690019 1103 92-93 -0.7955295145141328 1103 94-95 -1.2126972241232146 1103 96-97 -1.084558822758975 1103 98-99 -1.2485248002594602 1103 100 -1.0952089275984598 1104 1 0.20471856647048625 1104 2 0.10100322680085938 1104 3 -0.29535174854676427 1104 4 0.574903493081905 1104 5 0.29205406700066305 1104 6 0.673756208275428 1104 7 0.721605357912118 1104 8 0.7465626979147757 1104 9 0.3893186765638532 1104 10-11 0.33296824490064836 1104 12-13 0.3688420762986411 1104 14-15 0.5707898325441079 1104 16-17 0.14318499995550837 1104 18-19 0.6222306234229151 1104 20-21 0.7438600094504935 1104 22-23 0.46690294485177475 1104 24-25 0.3182263524754987 1104 26-27 0.4765498864346043 1104 28-29 0.7685168603860149 1104 30-31 0.37159095899485095 1104 32-33 0.5307807101321913 1104 34-35 0.9571918881771779 1104 36-37 1.0334815880054364 1104 38-39 0.5923616559224456 1104 40-41 0.17648832023397887 1104 42-43 0.6763128121337942 1104 44-45 0.46421609145726705 1104 46-47 1.0851396682428245 1104 48-49 0.9856734517640078 1104 50-51 0.8693454148854229 1104 52-53 0.761600549101864 1104 54-55 1.0301335013547757 1104 56-57 0.4985613637528843 1104 58-59 0.47317089587949823 1104 60-61 1.3366386223246707 1104 62-63 1.1101676059064758 1104 64-65 0.6691921214067662 1104 66-67 0.4464784146754113 1104 68-69 0.6768321393557599 1104 70-71 0.5067681560146298 1104 72-73 0.8785883588438992 1104 74-75 0.7707516969986941 1104 76-77 0.6453071687043277 1104 78-79 0.9098292203494829 1104 80-81 0.9675163821002677 1104 82-83 0.033463126854776704 1104 84-85 0.5212043202588461 1104 86-87 0.8171925025946862 1104 88-89 0.8524460689947837 1104 90-91 0.26574135365776996 1104 92-93 0.27836758850305543 1104 94-95 0.08286113625664271 1104 96-97 0.11525485676981972 1104 98-99 0.2916543078064109 1104 100 0.6548812274682554 1105 1 -0.45068065567547677 1105 2 -0.7422248674567484 1105 3 -0.8510026293520738 1105 4 -0.21050030467604586 1105 5 -0.9515972713658485 1105 6 -0.7492019248748818 1105 7 -0.8504033357506628 1105 8 -0.609648695359077 1105 9 -1.1248657205992671 1105 10-11 -1.042432121148316 1105 12-13 -0.9092979374281924 1105 14-15 -0.8150772459276396 1105 16-17 -1.2268390220074252 1105 18-19 -1.2899176264054972 1105 20-21 -1.300180255935011 1105 22-23 -1.5998151974497645 1105 24-25 -1.1902671272774157 1105 26-27 -1.1191947944164582 1105 28-29 -0.9206846953220662 1105 30-31 -1.2582992263174262 1105 32-33 -1.171404144592131 1105 34-35 -1.2046417883270522 1105 36-37 -0.8693724499721398 1105 38-39 -1.7187799592685877 1105 40-41 -2.2435242626989904 1105 42-43 -2.2110985353839325 1105 44-45 -1.1214336454450446 1105 46-47 -0.7735311164746292 1105 48-49 -1.1206237342346128 1105 50-51 -1.4098527091136575 1105 52-53 -1.076851750143156 1105 54-55 -1.6962163041817178 1105 56-57 -1.4152741429961395 1105 58-59 -1.016905745621301 1105 60-61 -1.5373604625529396 1105 62-63 -2.1421773952374323 1105 64-65 -1.914598773125384 1105 66-67 -1.9972987530207718 1105 68-69 -2.2068615234216367 1105 70-71 -1.7971096751452862 1105 72-73 -1.4216861778753866 1105 74-75 -1.445617554888745 1105 76-77 -1.333101662226813 1105 78-79 -1.642818915088629 1105 80-81 -1.814312718791978 1105 82-83 -1.9021923295625207 1105 84-85 -1.541910527602056 1105 86-87 -1.1465743699745232 1105 88-89 -1.3667829403852174 1105 90-91 -1.348820531494372 1105 92-93 -1.2406497988224992 1105 94-95 -1.1984818058618671 1105 96-97 -1.8042143722395636 1105 98-99 -1.3424969161697966 1105 100 -0.9873631102119091 1106 1 0.7424318116305386 1106 2 0.6518466233889377 1106 3 0.706756742923428 1106 4 0.883222450337037 1106 5 0.7827096161668479 1106 6 0.8534571858487467 1106 7 0.4229183730549195 1106 8 0.6715195696801572 1106 9 0.7129721172385715 1106 10-11 0.8048869721385259 1106 12-13 0.7628903799214086 1106 14-15 0.6005722745605979 1106 16-17 0.8831217452114117 1106 18-19 0.5432340736816883 1106 20-21 0.6650551407520027 1106 22-23 0.3935370257411748 1106 24-25 0.4087956451724537 1106 26-27 0.8503998001781383 1106 28-29 1.264036316011854 1106 30-31 1.167594409253617 1106 32-33 1.1031163436380353 1106 34-35 0.5358769562239445 1106 36-37 1.1835918046050224 1106 38-39 1.149027364183894 1106 40-41 0.7872080825494763 1106 42-43 0.6774149781295833 1106 44-45 1.0929299116604483 1106 46-47 1.1262073316509174 1106 48-49 1.3848252631498639 1106 50-51 0.4676538296832007 1106 52-53 1.0484751896999072 1106 54-55 0.8621969477799212 1106 56-57 1.0888670950160702 1106 58-59 1.0108841410395542 1106 60-61 0.9845445069326786 1106 62-63 1.4915410005777403 1106 64-65 1.3694029705537787 1106 66-67 0.7808180734831467 1106 68-69 1.3783607956716537 1106 70-71 0.9701091565896718 1106 72-73 0.7973635169807594 1106 74-75 0.6971461765846669 1106 76-77 0.6519680849397105 1106 78-79 0.3049796899680395 1106 80-81 0.5053254677816916 1106 82-83 0.3196908439335573 1106 84-85 0.02151580195330638 1106 86-87 0.6524666064858096 1106 88-89 0.8432214188126892 1106 90-91 0.5461227989328314 1106 92-93 0.5999125377075814 1106 94-95 0.5698268253499883 1106 96-97 0.9445868483358488 1106 98-99 0.6584839033594143 1106 100 0.5863073344262766 1107 1 0.12906844826717645 1107 2 0.30084562238793566 1107 3 0.1894394256061105 1107 4 0.15819742531201086 1107 5 0.35903594249317194 1107 6 0.4699737023652588 1107 7 0.6498953500318976 1107 8 0.6520000501606376 1107 9 0.7677268719933252 1107 10-11 0.8127948800464324 1107 12-13 0.46734483437586505 1107 14-15 0.6629096118979305 1107 16-17 0.5526412647309229 1107 18-19 -0.2627969573493445 1107 20-21 -0.052987902291043554 1107 22-23 0.6892827714869227 1107 24-25 0.407194043570847 1107 26-27 0.3030274278057661 1107 28-29 0.5050022819778164 1107 30-31 0.3939707856299961 1107 32-33 0.48397219949389125 1107 34-35 0.39383491418190886 1107 36-37 -0.055722509709291046 1107 38-39 -0.4455422053856779 1107 40-41 -0.3503044299630318 1107 42-43 -0.1898022390876335 1107 44-45 0.14538236411289773 1107 46-47 0.5100411654847505 1107 48-49 0.6074228607474623 1107 50-51 -0.17541423838486736 1107 52-53 0.06081252703724971 1107 54-55 -0.5739642133812382 1107 56-57 -0.38788465673567885 1107 58-59 0.1271504073058196 1107 60-61 -0.19070574331757228 1107 62-63 0.08195641599315451 1107 64-65 -0.31418061302980504 1107 66-67 0.21219945486453184 1107 68-69 -0.036879444568590714 1107 70-71 0.024813861294376238 1107 72-73 -0.5403241707069313 1107 74-75 0.30235138178986887 1107 76-77 -0.1784122954406726 1107 78-79 -0.0022275172391665876 1107 80-81 0.37854869100491584 1107 82-83 0.5660872403299528 1107 84-85 0.7066259120634157 1107 86-87 0.6910301700493733 1107 88-89 0.842595793187062 1107 90-91 0.5959225987326313 1107 92-93 0.04605868385372247 1107 94-95 -0.18002302449985663 1107 96-97 0.16663389538289763 1107 98-99 0.26864406351957015 1107 100 0.35953055764949937 1108 1 1.225686805755096 1108 2 0.45962178985975655 1108 3 1.1446729199700414 1108 4 0.8928190034118515 1108 5 1.0458314336364936 1108 6 0.6646596927034238 1108 7 0.7191385062315732 1108 8 0.6640773448466248 1108 9 0.9113661634587018 1108 10-11 1.218028374410359 1108 12-13 0.7068778456480089 1108 14-15 1.447370159401956 1108 16-17 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 1 2.0 2 1.0 3 1.0 4 2.0 5 0.0 6 7.0 7 152.0 8 458.0 9 734.0 10 938.0 11 995.0 12 1004.0 13 924.0 14 804.0 15 756.0 16 727.0 17 783.0 18 945.0 19 887.0 20 917.0 21 985.0 22 1103.0 23 1027.0 24 1093.0 25 1125.0 26 1274.0 27 1501.0 28 1717.0 29 2060.0 30 2383.0 31 2997.0 32 3810.0 33 5087.0 34 6826.0 35 9544.0 36 15215.0 37 24528.0 38 26533.0 39 6262.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.89862205507009 16.801704739717096 9.130376012785547 24.169297192427265 2 16.888624181060155 19.87135199523526 39.71729203891205 23.522731784792537 3 17.73486140302177 28.75441170638855 29.90918824602451 23.601538644565174 4 10.942172095716247 14.754007344484895 38.79014284467922 35.51367771511965 5 11.228657303504445 37.42198466260101 35.1245469757409 16.224811058153644 6 29.98152421279666 36.2847015724243 20.938696862287983 12.795077352491061 7 29.342172037553922 31.445064704389747 22.574378330373 16.63838492768333 8 29.590833399413214 33.057648085005155 20.382206010625644 16.96931250495599 9 30.19927205829877 11.560634054666995 18.816262122449626 39.423831764584605 10-11 25.20240426932257 24.718695731470394 30.88201476500488 19.196885234202156 12-13 24.990682663410226 23.25686509289583 29.822217288218923 21.930234955475026 14-15 22.48611270811113 24.666447271905444 26.155688337846783 26.691751682136637 16-17 22.53284321306353 26.914185333666424 27.5193014596543 23.033669993615746 18-19 22.385455698963103 26.96762425900593 27.948214675152165 22.698705366878805 20-21 23.279605067507287 26.883165804238786 27.516009437141893 22.321219691112038 22-23 22.998798386744976 26.44659562739678 27.47014804033931 23.084457945518935 24-25 22.59262930436334 26.439347382193763 27.895644588941977 23.072378724500922 26-27 22.360475029243243 26.55722754217966 27.69721842225262 23.38507900632447 28-29 22.6317897550394 26.553459468677005 27.678525398254305 23.13622537802929 30-31 23.32767631267992 26.653290720635702 27.780200956406574 22.2388320102778 32-33 22.915559644378177 26.538794026441643 27.88268603922626 22.662960289953922 34-35 23.43641576094499 26.72239118452931 27.377445052003825 22.46374800252188 36-37 23.045595501518793 26.768025252405085 27.61704220101993 22.569337045056194 38-39 23.130247185307685 26.941731209276686 27.263353677639902 22.664667927775728 40-41 23.16765087309488 26.813511058937372 27.461937187932534 22.556900880035215 42-43 22.802355787344585 26.877391976838723 27.307699934561462 23.01255230125523 44-45 22.558407044544047 27.359485938677565 27.407877513783667 22.674229502994724 46-47 22.733003972942754 26.306669944410523 27.22298438577988 23.73734169686685 48-49 22.53853444207838 26.75065529370244 27.543749033417004 23.167061230802176 50-51 22.64378390381626 26.42832217745773 27.917314341909083 23.010579576816927 52-53 22.869106958042373 26.78015568059703 27.116815963010986 23.23392139834961 54-55 22.36713232640858 27.01963225701563 27.773041433539397 22.840193983036396 56-57 22.77878225563433 26.992434695722512 27.14231336537089 23.08646968327227 58-59 22.517575127972307 26.85772968600691 27.414424094875955 23.210271091144833 60-61 23.152547212462025 27.269410449004194 27.369744366627273 22.208297971906504 62-63 22.780287197465114 27.010521055992005 27.689055801650532 22.52013594489235 64-65 22.59314184205935 26.705419594283814 27.324382623039224 23.377055940617613 66-67 22.735022401966614 26.929542841283055 27.2403949090044 23.095039847745927 68-69 22.598937267031484 27.04774367515267 27.355460385438974 22.99785867237687 70-71 22.772622729796176 26.34189864382584 27.53707669125228 23.348401935125704 72-73 22.167405633065506 27.18100843135544 27.236133473988115 23.41545246159093 74-75 22.537121644774412 27.11585443238784 27.224919093851135 23.12210482898661 76-77 22.374123253942134 26.64911522495054 27.64986459749971 23.326896923607617 78-79 22.587232355273592 26.88659793814433 27.197065820777162 23.329103885804916 80-81 22.359243589184388 26.976379014318514 26.887557446538533 23.776819949958565 82-83 22.91897165018975 26.884138144354424 27.265228753504566 22.931661451951253 84-85 22.24610040998232 27.272150543603246 26.756698889003435 23.725050157411005 86-87 22.502279846159944 26.956108005233737 27.40890527734824 23.13270687125808 88-89 22.419221800992972 27.009739384229814 27.360293767745823 23.21074504703139 90-91 22.71586335428356 27.126514265321095 27.312488847473087 22.84513353292226 92-93 22.702021103220204 26.858427607865533 27.32989940084619 23.109651888068075 94-95 22.50881248537861 26.569098212126143 27.354192522571463 23.567896779923792 96-97 22.753569743327425 26.984920040128635 27.369155672927842 22.892354543616097 98-99 22.729724156528082 26.77735831099408 27.339276366924164 23.15364116555367 100 23.007796698895135 26.4128046700878 27.180577257116568 23.398821373900493 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3.0 1 2.5 2 2.5 3 3.5 4 4.5 5 6.0 6 7.5 7 12.0 8 20.5 9 25.5 10 28.0 11 30.5 12 31.0 13 35.0 14 47.5 15 56.5 16 58.5 17 67.5 18 71.5 19 68.5 20 83.5 21 109.0 22 124.5 23 156.0 24 203.5 25 279.5 26 391.5 27 522.5 28 654.0 29 888.5 30 1146.0 31 1411.0 32 1774.0 33 2203.5 34 2637.5 35 2966.0 36 3390.0 37 3896.0 38 4189.0 39 4388.5 40 4473.0 41 4452.0 42 4421.0 43 4392.5 44 4489.5 45 4751.5 46 4885.0 47 4708.0 48 4661.5 49 4669.0 50 4655.5 51 4526.0 52 4296.5 53 4131.5 54 3897.5 55 3605.5 56 3305.5 57 3058.5 58 2783.0 59 2466.5 60 2030.5 61 1641.5 62 1365.5 63 1088.0 64 870.0 65 727.0 66 626.5 67 546.0 68 459.5 69 355.0 70 295.0 71 251.5 72 212.5 73 186.5 74 164.5 75 138.0 76 117.5 77 96.0 78 75.5 79 54.5 80 39.5 81 36.0 82 33.0 83 22.5 84 15.0 85 13.0 86 10.0 87 7.5 88 4.0 89 1.5 90 2.0 91 2.0 92 0.5 93 0.0 94 0.5 95 1.5 96 1.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2759386274432066 2 0.15065614716726797 3 0.023787812710621258 4 0.02695952107203743 5 0.014272687626372755 6 0.003171708361416168 7 0.002378781271062126 8 0.00396463545177021 9 0.004757562542124252 10-11 0.004757562542124252 12-13 0.004757562542124252 14-15 0.00832573444871744 16-17 0.018633786623319985 18-19 0.013876224081195735 20-21 0.013876224081195735 22-23 0.02735598461721445 24-25 0.0059469531776553146 26-27 0.013876224081195735 28-29 0.026563057526860407 30-31 0.014272687626372755 32-33 0.020616104349205088 34-35 0.014669151171549776 36-37 0.022201958529913177 38-39 0.02814891170756849 40-41 0.032113547159338694 42-43 0.03369940134004678 44-45 0.047575625421242516 46-47 0.009515125084248503 48-49 0.021012567894382113 50-51 0.018237323078142964 52-53 0.018633786623319985 54-55 0.0170479324426119 56-57 0.010308052174602544 58-59 0.010704515719779567 60-61 0.02854537525274551 62-63 0.02735598461721445 64-65 0.013479760536018714 66-67 0.00792927090354042 68-69 0.01982317725885105 70-71 0.01982317725885105 72-73 0.030131229433453595 74-75 0.0340958648852238 76-77 0.007532807358363399 78-79 0.011893906355310629 80-81 0.015462078261903819 82-83 0.023391349165444237 84-85 0.009515125084248503 86-87 0.00792927090354042 88-89 0.022994885620267216 90-91 0.017840859532965946 92-93 0.016255005352257858 94-95 0.012290369900487649 96-97 0.016255005352257858 98-99 0.022994885620267216 100 0.02854537525274551 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 126115.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.9375173452801 #Duplication Level Percentage of deduplicated Percentage of total 1 79.0518495657158 56.867937993101535 2 14.669767646929147 21.10613329104389 3 3.744323442528989 8.080719977798042 4 1.1375159825404524 3.2732030289814853 5 0.43318195846743973 1.5581017325456925 6 0.21824434548741237 0.9419973833406018 7 0.12124685860411798 0.6105538595726123 8 0.09920197522155108 0.5709075050549103 9 0.09148626603765266 0.5923165364944694 >10 0.41113707508487285 5.168298774927646 >50 0.01873815087518187 0.8841137057447568 >100 0.003306732507385036 0.3457162113943623 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACCTGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 219 0.1736510327875352 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.002378781271062126 16-17 0.0 0.0 0.0 0.0 0.002378781271062126 18-19 0.0 0.0 0.0 0.0 0.002378781271062126 20-21 0.0 0.0 0.0 0.0 0.002378781271062126 22-23 0.0 0.0 0.0 0.0 0.002378781271062126 24-25 0.0 0.0 0.0 3.96463545177021E-4 0.002378781271062126 26-27 0.0 0.0 0.0 7.92927090354042E-4 0.002378781271062126 28-29 0.0 0.0 0.0 0.00396463545177021 0.002378781271062126 30-31 0.0 0.0 0.0 0.006739880268009356 0.002378781271062126 32-33 0.0 0.0 0.0 0.010308052174602546 0.002378781271062126 34-35 0.0 0.0 0.0 0.013876224081195734 0.002378781271062126 36-37 0.0 0.0 0.0 0.021409031439559134 0.002378781271062126 38-39 0.0 0.0 0.0 0.04083574515323316 0.002378781271062126 40-41 0.0 0.0 0.0 0.08048209967093525 0.002378781271062126 42-43 0.0 0.0 0.0 0.10426991238155651 0.002378781271062126 44-45 0.0 0.0 0.0 0.13519406890536415 0.002378781271062126 46-47 0.0 0.0 0.0 0.17721920469412838 0.002378781271062126 48-49 0.0 0.0 0.0 0.2251912936605479 0.002378781271062126 50-51 0.0 0.0 0.0 0.264044721087896 0.002378781271062126 52-53 0.0 0.0 0.0 0.3076557110573683 0.002378781271062126 54-55 0.0 0.0 0.0 0.36236768029179717 0.002378781271062126 56-57 0.0 0.0 0.0 0.42183721206835034 0.002378781271062126 58-59 0.0 0.0 0.0 0.49557943147127625 0.002378781271062126 60-61 0.0 0.0 0.0 0.5590135986995997 0.002378781271062126 62-63 0.0 0.0 0.0 0.624430083653808 0.002378781271062126 64-65 0.0 0.0 0.0 0.6862783967014233 0.002378781271062126 66-67 0.0 0.0 0.0 0.7770685485469611 0.002378781271062126 68-69 0.0 0.0 0.0 0.8968005391904215 0.002378781271062126 70-71 0.0 0.0 0.0 1.0403203425445031 0.002378781271062126 72-73 0.0 0.0 0.0 1.1576735519169012 0.002378781271062126 74-75 0.0 0.0 0.0 1.2567894382111566 0.002378781271062126 76-77 0.0 0.0 0.0 1.360662887047536 0.002378781271062126 78-79 0.0 0.0 0.0 1.494667565317369 0.002378781271062126 80-81 0.0 0.0 0.0 1.6369979780359196 0.002378781271062126 82-83 0.0 0.0 0.0 1.7650557031280973 0.002378781271062126 84-85 0.0 0.0 0.0 1.887959402132974 0.002378781271062126 86-87 0.0 0.0 0.0 1.9922293145145304 0.002378781271062126 88 0.0 0.0 0.0 2.0861911747214843 0.002378781271062126 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 450 0.0 80.51702 2 GTACCTG 480 0.0 76.617165 1 ACCTGGG 520 0.0 67.787605 3 GTACACG 30 1.3452017E-4 62.865364 1 CCTGGGG 310 0.0 62.1605 4 ATAACGC 25 0.005225524 56.39929 3 CTGGGGG 240 0.0 45.023235 5 GTACCCG 45 9.948883E-4 41.910244 1 GTATCAT 60 8.617059E-5 39.29085 1 CCTGGGA 270 0.0 38.29581 4 GTTAGAG 50 0.0016963907 37.599525 3 TAGCTAA 25 0.0016996139 37.599525 60-61 AATACTG 65 1.4097567E-4 36.139053 5 GTTATAC 55 0.002666145 34.2902 1 GTACATG 505 0.0 31.743898 1 GGGTTAG 60 0.0040832623 31.432682 1 GTATATG 60 0.004147328 31.332935 3 ATCATGC 60 0.004147328 31.332935 3 CTAATAT 60 0.004147328 31.332935 3 TGGGGGG 200 0.0 30.5375 6 >>END_MODULE