FastQCFastQC Report
Mon 6 Jun 2016
ERR1211643_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211643_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126115
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACCTGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG1280.10149466756531737No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA255.557262E-575.153374
GTACCTG4050.070.971361
ACCTGGG4050.069.586463
TACCTGG4250.067.443732
CCTGGGG3100.056.0619854
GGGCCTA352.9364522E-453.6809777
ATAACGC352.9364522E-453.6809773
TTACTCT405.674727E-446.9708564
TATAGCT555.2755495E-542.700784
TATACAG555.2755495E-542.700785
GTGTATA459.978749E-441.8847351
ATACTCC500.001701463537.5766876
CTGGGGG1800.036.5328875
GAGTACC651.3853492E-436.2464071
TGGGGGC801.15540115E-535.228146
ACATGGG5950.034.7347533
GCATAGG702.1415918E-433.6573751
GTACATG6450.033.6051941
TACATGG6300.033.563922
CCTGGGA2250.033.4014974
Produced by FastQC (version 0.11.5)