##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211643_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 126115 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.93858779685208 33.0 31.0 34.0 30.0 34.0 2 32.19276057566507 33.0 31.0 34.0 30.0 34.0 3 32.29208262300281 34.0 31.0 34.0 30.0 34.0 4 35.96512706656623 37.0 35.0 37.0 35.0 37.0 5 35.88052967529636 37.0 35.0 37.0 35.0 37.0 6 35.89961543036118 37.0 35.0 37.0 35.0 37.0 7 35.809348610395276 37.0 35.0 37.0 35.0 37.0 8 35.77949490544344 37.0 35.0 37.0 33.0 37.0 9 37.40931689331166 39.0 37.0 39.0 34.0 39.0 10-11 37.343345359394206 39.0 37.0 39.0 34.0 39.0 12-13 37.23630813146731 39.0 37.0 39.0 34.0 39.0 14-15 38.46182056059945 40.0 38.0 41.0 34.0 41.0 16-17 38.39113903976529 40.0 38.0 41.0 33.5 41.0 18-19 38.355711057368275 40.0 38.0 41.0 33.5 41.0 20-21 38.274214011021684 40.0 38.0 41.0 33.5 41.0 22-23 38.150897989929824 40.0 37.5 41.0 33.0 41.0 24-25 38.041458192919166 40.0 37.0 41.0 33.0 41.0 26-27 37.8436823534076 40.0 37.0 41.0 33.0 41.0 28-29 37.85981445506086 40.0 37.0 41.0 33.0 41.0 30-31 37.77068945010507 40.0 37.0 41.0 32.5 41.0 32-33 37.5964318280934 40.0 37.0 41.0 32.0 41.0 34-35 37.445014470919396 40.0 37.0 41.0 31.5 41.0 36-37 37.357796455615905 40.0 37.0 41.0 31.0 41.0 38-39 37.301399516314476 40.0 36.5 41.0 31.0 41.0 40-41 37.1539745470404 39.5 36.0 41.0 31.0 41.0 42-43 36.985929508781666 39.0 36.0 41.0 30.0 41.0 44-45 36.823105895412915 39.0 36.0 41.0 30.0 41.0 46-47 36.63768385996907 39.0 35.0 41.0 30.0 41.0 48-49 36.466942869603145 39.0 35.0 40.5 29.5 41.0 50-51 36.30633548745193 39.0 35.0 40.0 29.0 41.0 52-53 36.109598382428736 39.0 35.0 40.0 28.0 41.0 54-55 35.8854339293502 38.0 34.5 40.0 28.0 41.0 56-57 35.660028545375255 38.0 34.0 40.0 27.5 41.0 58-59 35.410454743686316 38.0 34.0 40.0 26.5 41.0 60-61 35.07489592831939 38.0 34.0 40.0 26.0 41.0 62-63 34.712631328549335 37.0 33.0 40.0 26.0 41.0 64-65 34.37121674662015 37.0 33.0 39.5 25.5 41.0 66-67 34.0294453475003 36.0 32.0 39.0 25.0 40.5 68-69 33.63327914998216 36.0 32.0 39.0 24.0 40.0 70-71 33.2655076715696 35.0 31.5 38.5 23.5 40.0 72-73 32.816520635927525 35.0 31.0 37.5 22.5 40.0 74-75 32.434690560202995 35.0 31.0 37.0 22.0 39.0 76-77 30.94510565753479 33.5 29.5 35.5 21.0 38.0 78-79 31.78070015462078 34.0 30.5 36.0 22.0 38.5 80-81 31.755845854973636 34.0 31.0 36.0 22.0 37.5 82-83 31.462181342425563 34.0 30.0 35.0 21.0 37.0 84-85 31.139428299567854 34.0 30.0 35.0 20.0 36.5 86-87 30.869959957181937 34.0 30.0 35.0 20.0 36.0 88-89 30.580339372794672 34.0 29.0 35.0 20.0 36.0 90-91 30.275530269991677 34.0 29.0 35.0 19.0 35.5 92-93 30.03111049439004 34.0 29.0 35.0 18.0 35.0 94-95 29.795595290013082 34.0 29.0 35.0 18.0 35.0 96-97 29.55883519010427 33.0 29.0 35.0 17.5 35.0 98-99 29.37623597510209 33.0 27.0 35.0 17.0 35.0 100 27.562518336438963 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.27233043224811126 1101 2 0.3457488195149665 1101 3 0.22940885021529311 1101 4 0.26943734956790166 1101 5 0.16559581004664636 1101 6 0.14744437404448973 1101 7 0.22952505923276334 1101 8 0.5042246768133367 1101 9 0.19924412268165526 1101 10-11 0.24924185857042858 1101 12-13 0.3422319555090567 1101 14-15 0.6459230494391335 1101 16-17 0.2229260200718386 1101 18-19 0.12850902584698787 1101 20-21 0.5668064112351985 1101 22-23 0.3410060653725395 1101 24-25 0.3677582740547862 1101 26-27 0.7188130569250148 1101 28-29 0.4238604479833441 1101 30-31 0.4523643982658143 1101 32-33 0.35030251984412075 1101 34-35 0.46317129681995794 1101 36-37 0.1092200267162795 1101 38-39 0.0722127770250367 1101 40-41 0.0848938850261618 1101 42-43 -0.066904793812121 1101 44-45 0.07854731140422189 1101 46-47 -0.16038470796020476 1101 48-49 0.3779782130178475 1101 50-51 0.0571305430407989 1101 52-53 0.06973996630352275 1101 54-55 -0.44520747768338254 1101 56-57 -0.3656928915333353 1101 58-59 -0.15814927280257507 1101 60-61 0.4229523122494214 1101 62-63 -0.0016117202902208305 1101 64-65 -0.027422494303813494 1101 66-67 0.20371489016523725 1101 68-69 -0.6834201809266389 1101 70-71 -0.3576933981596966 1101 72-73 -0.9639478301122679 1101 74-75 -0.8988988332268661 1101 76-77 -0.26447986511599453 1101 78-79 0.06364435568314519 1101 80-81 0.07613985686250047 1101 82-83 -0.17455117575144286 1101 84-85 -0.4810047221328766 1101 86-87 -0.41030256982352853 1101 88-89 -0.5493267167230513 1101 90-91 -0.3244164081141534 1101 92-93 -1.0249026228425429 1101 94-95 -1.2760554510990332 1101 96-97 -0.42340123471386804 1101 98-99 -0.5776645352483456 1101 100 -0.5058303023917148 1102 1 0.420295765428385 1102 2 0.22527588144460253 1102 3 0.16920107887855806 1102 4 0.25095603176959 1102 5 0.3453581931035288 1102 6 0.45525588756110835 1102 7 0.5146653909781591 1102 8 0.4702466018549458 1102 9 0.3259731590129391 1102 10-11 0.37272805497747896 1102 12-13 0.46566075651921324 1102 14-15 0.45697740287998556 1102 16-17 0.6613407592097644 1102 18-19 0.5427890005930109 1102 20-21 0.8435957127332117 1102 22-23 0.3003414266765603 1102 24-25 0.5574500607734905 1102 26-27 0.6547310962982564 1102 28-29 0.8451139634514035 1102 30-31 0.7501891127237101 1102 32-33 0.6109637148162861 1102 34-35 0.28837361559399 1102 36-37 0.6968914954644916 1102 38-39 0.37167326029427983 1102 40-41 0.5177699683642771 1102 42-43 0.6489305961896008 1102 44-45 0.1663335709462075 1102 46-47 0.4904503950645278 1102 48-49 0.47807578519255145 1102 50-51 0.7558334070711012 1102 52-53 0.5487045533436472 1102 54-55 0.5145296513988669 1102 56-57 0.42016488267316987 1102 58-59 0.636203020143526 1102 60-61 0.017970391799444485 1102 62-63 0.46237266816182654 1102 64-65 0.1422382989005655 1102 66-67 0.5025727217671445 1102 68-69 0.48331344703640156 1102 70-71 0.23210170027340382 1102 72-73 -0.0018000943606750752 1102 74-75 -0.1496880199340893 1102 76-77 -0.12675961028043048 1102 78-79 0.1873314359793028 1102 80-81 0.6356301857381261 1102 82-83 -0.024376119790932904 1102 84-85 -0.18659503378988163 1102 86-87 -0.27763310991421264 1102 88-89 0.16963672240912686 1102 90-91 0.27857963160346344 1102 92-93 0.1754360099991068 1102 94-95 -0.003771688256811956 1102 96-97 -0.2028594221672364 1102 98-99 0.016176938665449114 1102 100 0.11822984798422453 1103 1 0.4171098344993709 1103 2 0.1987052174965953 1103 3 0.09847341225436423 1103 4 0.2861924278445258 1103 5 0.06047224943078788 1103 6 0.35419815849267167 1103 7 0.3100011880980773 1103 8 -0.20446985029236942 1103 9 0.25384368277998703 1103 10-11 0.13480273319845537 1103 12-13 0.25787434132869436 1103 14-15 -0.07150481962543154 1103 16-17 0.4919129524046113 1103 18-19 0.618805312559374 1103 20-21 0.10465089305690611 1103 22-23 0.3190109819205986 1103 24-25 0.5203976840690174 1103 26-27 0.3938033532117302 1103 28-29 0.4838293961008304 1103 30-31 0.15177570632636872 1103 32-33 -0.0318297094222757 1103 34-35 0.542515325801709 1103 36-37 0.01988117304827597 1103 38-39 0.22362305002283023 1103 40-41 -0.0017279289213121274 1103 42-43 -0.32402085022304306 1103 44-45 0.3951471302682421 1103 46-47 -0.4815452720694111 1103 48-49 -0.09265188143162817 1103 50-51 0.1564642213950478 1103 52-53 0.46749517396299467 1103 54-55 -0.029468054730863003 1103 56-57 -0.21632039832594074 1103 58-59 -0.27016246985265013 1103 60-61 -0.22444709258949302 1103 62-63 -0.6847854814494951 1103 64-65 -1.1009119496020432 1103 66-67 -0.8290189693249914 1103 68-69 -0.7142779891812623 1103 70-71 -0.49648884388359704 1103 72-73 -0.4556996738177972 1103 74-75 -0.2628204272235024 1103 76-77 0.1735780983980426 1103 78-79 0.08968265300492106 1103 80-81 0.27377215082032436 1103 82-83 0.5748019100293824 1103 84-85 0.18198531538814677 1103 86-87 -0.06659704517609555 1103 88-89 0.8422504601432941 1103 90-91 0.5799049788699335 1103 92-93 0.41202324078794206 1103 94-95 -0.48274454412529977 1103 96-97 -1.0412690054474716 1103 98-99 -0.6931387233709998 1103 100 -0.6075252176459536 1104 1 0.23968466320474136 1104 2 -0.27208454088782474 1104 3 -0.054549787649470716 1104 4 -0.03758446841704455 1104 5 0.09851768758441182 1104 6 0.23747739878597685 1104 7 0.1629883259752276 1104 8 -0.27304780437339105 1104 9 -0.21015439391192814 1104 10-11 -0.06906792433250075 1104 12-13 -0.24585206809551607 1104 14-15 -0.24165748220507055 1104 16-17 0.035307591183311615 1104 18-19 0.14266635354987756 1104 20-21 0.12045808142089243 1104 22-23 -0.12737680507302684 1104 24-25 -0.007099611278981399 1104 26-27 -0.06923306943522078 1104 28-29 0.03828305639653706 1104 30-31 -0.09335592007104054 1104 32-33 -0.005444326563761592 1104 34-35 -0.6520092564798077 1104 36-37 -0.20424432280459115 1104 38-39 -0.28848778060584834 1104 40-41 -0.40189621837917855 1104 42-43 0.10707062711798443 1104 44-45 0.6379647766199668 1104 46-47 0.5190858133975951 1104 48-49 -0.07191621608723864 1104 50-51 0.05455894431847952 1104 52-53 -0.16422137850725704 1104 54-55 -0.6634685355578824 1104 56-57 -0.29409416921186704 1104 58-59 -0.37627498337590737 1104 60-61 -0.14664327001466404 1104 62-63 -0.7565789662433389 1104 64-65 -0.2485258455029964 1104 66-67 -0.5747215778115873 1104 68-69 -0.15591760932791487 1104 70-71 -0.14341155093973157 1104 72-73 -0.08218723241859038 1104 74-75 -0.12779818897371342 1104 76-77 0.3531369396049193 1104 78-79 0.7072749116898507 1104 80-81 0.12621835829718364 1104 82-83 0.08336664136728089 1104 84-85 -0.29815051824570205 1104 86-87 0.13739381911047843 1104 88-89 -0.037903404503897065 1104 90-91 0.15779895651147768 1104 92-93 -0.12259630483051964 1104 94-95 -0.12308593131125889 1104 96-97 -0.3938955230577612 1104 98-99 -1.1244405625343603 1104 100 -2.0306529974271754 1105 1 0.20073476615601038 1105 2 0.003653276545257711 1105 3 0.023979153096355788 1105 4 -0.05634447756826688 1105 5 -0.1785928134450998 1105 6 -0.35083191258441815 1105 7 -0.5667073958275637 1105 8 -0.12628504985497102 1105 9 0.07027342580896345 1105 10-11 -0.0026357577802258447 1105 12-13 -0.22394632570247097 1105 14-15 -0.27783341448601107 1105 16-17 -0.03781640790156615 1105 18-19 0.2426434754899347 1105 20-21 0.2757143156922197 1105 22-23 0.07632372112234975 1105 24-25 -0.06612500592551385 1105 26-27 -0.37328248604469394 1105 28-29 -0.19584528951973112 1105 30-31 0.4633530710065159 1105 32-33 0.14798166291233628 1105 34-35 0.39557710819646275 1105 36-37 0.11679205331071074 1105 38-39 -0.8039876662155478 1105 40-41 0.16954052378508777 1105 42-43 0.028084125173343466 1105 44-45 0.3719787322365278 1105 46-47 0.1564399657654718 1105 48-49 0.6796280529587477 1105 50-51 0.9402258397657448 1105 52-53 0.5983043593101698 1105 54-55 0.4216664449957648 1105 56-57 -0.013408971316643203 1105 58-59 0.3961169177908701 1105 60-61 0.2537513765193111 1105 62-63 0.18802753112567672 1105 64-65 -0.43046361717996007 1105 66-67 0.32776069169195665 1105 68-69 0.3317854334540584 1105 70-71 0.25480967989989267 1105 72-73 0.19629595220735752 1105 74-75 -0.19734749119288608 1105 76-77 -0.6343087250027217 1105 78-79 -0.6143906110738122 1105 80-81 0.3024080174140913 1105 82-83 0.11854165852582454 1105 84-85 0.17385245590208953 1105 86-87 0.31141006253303516 1105 88-89 -0.19793543378781564 1105 90-91 -0.28566363786052307 1105 92-93 -0.871738377582755 1105 94-95 -0.9796690930591438 1105 96-97 -0.2533384422982081 1105 98-99 -0.115432326432785 1105 100 -0.09453997981107065 1106 1 0.5444575211690932 1106 2 0.3545687081058517 1106 3 0.25937584010594605 1106 4 0.3917810673314719 1106 5 0.35628076981557655 1106 6 0.4817748877643169 1106 7 0.2318976608420087 1106 8 0.06225025964807429 1106 9 0.4037520657392122 1106 10-11 0.812425270990488 1106 12-13 0.7563283037657058 1106 14-15 0.5607672829856511 1106 16-17 0.8302088754462957 1106 18-19 0.6038030221333557 1106 20-21 0.6422138797724344 1106 22-23 0.5354562407387391 1106 24-25 0.36401884760543624 1106 26-27 0.47087618875914217 1106 28-29 0.6061381455282202 1106 30-31 0.47730250431079213 1106 32-33 0.3058936071145979 1106 34-35 0.2107471169148667 1106 36-37 0.05085918495849029 1106 38-39 0.37822680632149996 1106 40-41 0.41169396755143595 1106 42-43 0.2557432358969791 1106 44-45 0.38930654391918296 1106 46-47 0.5593388323740243 1106 48-49 0.5682505464199465 1106 50-51 0.9566818118668721 1106 52-53 0.8668309504174161 1106 54-55 0.7489332279077061 1106 56-57 0.9850653094156954 1106 58-59 0.4703322621675099 1106 60-61 0.3531410888122437 1106 62-63 0.5019987604194895 1106 64-65 0.8394797132472434 1106 66-67 0.3615444754757391 1106 68-69 0.5597714840207502 1106 70-71 0.2927347016958777 1106 72-73 -0.10950178100973318 1106 74-75 0.5891607912831418 1106 76-77 0.46824140577321316 1106 78-79 0.1807270872696911 1106 80-81 0.4646791595239428 1106 82-83 0.45060355913611616 1106 84-85 0.7526449580816887 1106 86-87 0.3919462438756689 1106 88-89 0.45768793151296805 1106 90-91 0.6329283412153295 1106 92-93 0.0881570016674651 1106 94-95 0.5504093725031964 1106 96-97 0.7493860563940338 1106 98-99 0.6695502367755886 1106 100 0.45362915706774487 1107 1 0.43795101466258046 1107 2 0.4342984378355794 1107 3 0.1033698340999436 1107 4 0.2404297159801203 1107 5 0.3346090981439076 1107 6 -0.016423310433879124 1107 7 0.16913489807924265 1107 8 0.14097898837680845 1107 9 0.27857689056403956 1107 10-11 0.13329614186135785 1107 12-13 0.06321018564758418 1107 14-15 0.09214866436703062 1107 16-17 0.29787654311395784 1107 18-19 0.41886808719841895 1107 20-21 0.186257923816477 1107 22-23 0.10162240690490876 1107 24-25 0.06744727603386025 1107 26-27 0.6184737863567378 1107 28-29 0.1872192266093009 1107 30-31 0.2503005023087894 1107 32-33 0.04908680030779777 1107 34-35 0.3446810508487985 1107 36-37 0.6486319577312614 1107 38-39 0.3067052847999818 1107 40-41 0.37485713071460225 1107 42-43 -0.11940691425316885 1107 44-45 0.14781505242768844 1107 46-47 0.4886681617033446 1107 48-49 0.3826149107843122 1107 50-51 0.5301302603153246 1107 52-53 0.15051463410109278 1107 54-55 0.0013856803601584033 1107 56-57 0.39014538949577826 1107 58-59 0.3403522821875242 1107 60-61 0.11017312084427289 1107 62-63 0.21631307473381156 1107 64-65 0.31057580934334084 1107 66-67 1.0296125435438128 1107 68-69 0.7414031156523819 1107 70-71 0.756273240234421 1107 72-73 0.3318395603316091 1107 74-75 0.32547210259445336 1107 76-77 -0.126453288921482 1107 78-79 0.4332796398222456 1107 80-81 0.04150598635076719 1107 82-83 0.4583863419188994 1107 84-85 0.6027951082318346 1107 86-87 -0.1303760784466519 1107 88-89 0.6339642077892442 1107 90-91 0.47482023310722354 1107 92-93 0.39576460927507284 1107 94-95 -0.1284444813506589 1107 96-97 0.7444811514891292 1107 98-99 0.9183490355743835 1107 100 1.1682437716823593 1108 1 0.4960830597511503 1108 2 -0.05700590999050803 1108 3 0.19660654907578845 1108 4 -0.19145434199089806 1108 5 -0.03962600652597814 1108 6 0.1531419069574227 1108 7 0.0029513232000155654 1108 8 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0.002368888040045647 2316 62-63 -0.0359356025148756 2316 64-65 -1.5271226033550747 2316 66-67 -1.48652502259376 2316 68-69 -0.8206339213846547 2316 70-71 -0.6184622095010326 2316 72-73 -1.6096948312027806 2316 74-75 -1.2692368921145416 2316 76-77 -0.28108291855111034 2316 78-79 -0.6468790903364834 2316 80-81 -0.13203898719420692 2316 82-83 -0.1700528115202573 2316 84-85 0.19009669553342334 2316 86-87 -0.14135491442549153 2316 88-89 -0.8025437287186925 2316 90-91 -0.9501797668927807 2316 92-93 -0.8130978245873628 2316 94-95 -0.13704236494854172 2316 96-97 -0.1466564146484366 2316 98-99 0.40565062287597087 2316 100 -1.127258873820285 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 1.0 13 5.0 14 13.0 15 47.0 16 121.0 17 263.0 18 392.0 19 508.0 20 668.0 21 760.0 22 901.0 23 1053.0 24 1304.0 25 1536.0 26 1694.0 27 2161.0 28 2624.0 29 3164.0 30 3659.0 31 4421.0 32 5332.0 33 6729.0 34 8669.0 35 11391.0 36 16461.0 37 24711.0 38 23986.0 39 3539.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.60461787997328 17.969023312024934 9.525172534427377 23.901186273574403 2 16.10988557553443 21.102664614114996 39.43279744806462 23.35465236228595 3 16.412927985790635 30.019664747767894 30.671455984268203 22.89595128217327 4 10.429535416650147 16.09746814364895 39.01818210652351 34.45481433317739 5 10.709273282321691 38.157237442017205 35.53582048130674 15.597668794354359 6 29.253923068993682 37.58216837280851 20.95501653279202 12.208892025405788 7 28.809419973833407 31.66633628037902 22.97347658882766 16.550767156959918 8 29.262413372028483 33.880457363972276 20.56472715162472 16.292402112374518 9 29.570629980573287 12.138127899139674 19.49411251635412 38.797129603932916 10-11 25.14173571740079 25.307853942829954 30.808785632161122 18.741624707608135 12-13 24.664486073941916 23.883040935672515 30.279314104470217 21.173158885915353 14-15 22.382438181176628 25.21438851202677 26.487438002767327 25.915735304029276 16-17 22.381160052333186 27.49276454030052 27.559766879435436 22.566308527930858 18-19 22.00209336071618 27.41033834735515 28.324597781354814 22.26297051057385 20-21 23.04984081546885 27.17515849070069 27.950266232659192 21.824734461171268 22-23 22.739959560718393 26.94168021250446 27.87773064266741 22.440629584109743 24-25 22.42199579748642 27.22634103794156 27.79645561590612 22.5552075486659 26-27 22.16389802957618 27.08559647940372 28.163977322285216 22.586528168734883 28-29 22.449707801733364 26.804928912959014 28.271470823785016 22.473892461522603 30-31 23.15880869689329 26.941893842079388 28.26173144932363 21.637566011703697 32-33 22.851818812568144 27.04212508672812 28.051541282584992 22.054514818118744 34-35 22.991344891979654 27.322467201382917 27.79150031123499 21.894687595402445 36-37 22.976921582542708 27.172745185884146 27.70282325048468 22.147509981088465 38-39 22.99701852321746 27.362978939355493 27.747557726465367 21.892444810961685 40-41 22.712584798011235 27.019590273455 28.185649658825536 22.082175269708227 42-43 22.46831929707697 27.300122123360453 27.829059015717434 22.40249956384514 44-45 22.500426227038258 27.439109007069423 27.70594695753193 22.354517808360395 46-47 22.455485114127008 27.229510861592505 27.356781209980213 22.95822281430027 48-49 22.297819700015463 27.309060199117415 27.895072815437743 22.49804728542938 50-51 22.270373571649472 27.277232826092128 27.91994100248202 22.53245259977638 52-53 22.597859830388188 27.125995646709463 27.582337851821602 22.69380667108075 54-55 22.36103897133749 27.215307817660612 28.307647168442294 22.1160060425596 56-57 22.78407739275236 27.203631750059472 27.466497502180637 22.545793355007532 58-59 22.487461591832687 27.075428684706115 27.875507978987013 22.56160174447418 60-61 22.956357624034034 27.79163312966627 27.422891332188783 21.829117914110917 62-63 22.855828743849298 27.163797991332515 27.870756957578497 22.109616307239687 64-65 22.698761359491222 26.90950470239322 27.637860212836816 22.753873725278734 66-67 22.56042439813493 27.248073080216955 27.506581660164304 22.684920861483807 68-69 22.51268834258525 27.26566217287867 27.559476605868362 22.662172878667725 70-71 22.4211210758776 26.77720420899049 27.824694118673232 22.976980596458677 72-73 21.97312439779698 27.870451508530962 27.22453300766458 22.93189108600748 74-75 22.245879474888287 27.49778956675508 27.42959324063391 22.826737717722718 76-77 22.21966711337018 27.132877114242444 27.921084141496642 22.72637163089074 78-79 22.299545182383053 27.16771944850886 27.584866905376522 22.947868463731567 80-81 21.982570633341 27.453234899967487 27.34459872015479 23.219595746536726 82-83 22.367920189059305 27.30534980729274 27.70345286999001 22.62327713365795 84-85 22.05615259547131 27.621842407899432 27.377562755284135 22.944442241345126 86-87 22.11485082557457 27.687280917410824 27.47156882960331 22.726299427411295 88-89 21.971616525700963 27.32236536883052 27.6677412575489 23.03827684791962 90-91 22.649523499807657 27.6056807230588 27.18926357619046 22.555532200943084 92-93 22.301975682685217 27.509259773323926 27.325650563517684 22.86311398047318 94-95 22.131316576377927 27.059257437914923 27.538726333907054 23.27069965180009 96-97 22.246542316964852 27.511975003172186 27.60714059129552 22.63434208856744 98-99 21.993996518456544 27.29641099660173 27.359459448742403 23.350133036199328 100 21.389258977287145 27.977889861692677 27.17532673518589 23.457524425834286 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2.0 1 1.0 2 10.0 3 15.5 4 10.5 5 13.5 6 19.0 7 20.5 8 22.5 9 32.5 10 47.5 11 53.0 12 51.5 13 57.0 14 66.5 15 70.5 16 77.5 17 82.0 18 89.0 19 94.5 20 121.0 21 137.5 22 145.0 23 178.0 24 227.0 25 311.5 26 451.5 27 599.5 28 743.5 29 915.0 30 1157.5 31 1478.5 32 1881.5 33 2249.5 34 2634.5 35 3061.0 36 3575.5 37 4078.0 38 4333.5 39 4594.0 40 4604.0 41 4534.5 42 4506.5 43 4519.0 44 4675.5 45 4803.5 46 4915.5 47 4869.0 48 4801.0 49 4822.5 50 4837.0 51 4720.0 52 4397.5 53 4090.0 54 3918.5 55 3610.5 56 3285.0 57 3051.0 58 2770.0 59 2367.5 60 1820.0 61 1399.0 62 1120.0 63 864.0 64 648.5 65 516.0 66 428.0 67 334.5 68 272.5 69 217.0 70 163.0 71 128.0 72 112.0 73 86.5 74 60.5 75 43.0 76 29.0 77 21.0 78 13.5 79 7.0 80 5.0 81 6.0 82 5.0 83 2.0 84 2.5 85 1.5 86 0.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2719739919914364 2 0.0737422194029259 3 7.92927090354042E-4 4 0.001585854180708084 5 0.0 6 0.001585854180708084 7 0.0 8 7.92927090354042E-4 9 0.0 10-11 0.0 12-13 0.001982317725885105 14-15 3.96463545177021E-4 16-17 0.0 18-19 0.001585854180708084 20-21 0.002775244816239147 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.001585854180708084 30-31 7.92927090354042E-4 32-33 0.001982317725885105 34-35 0.0035681719065931887 36-37 0.001189390635531063 38-39 0.002378781271062126 40-41 0.005154026087301272 42-43 0.010308052174602544 44-45 0.006739880268009357 46-47 0.00436109899694723 48-49 0.006739880268009357 50-51 0.006343416722832336 52-53 0.002775244816239147 54-55 0.007532807358363399 56-57 0.00396463545177021 58-59 0.001982317725885105 60-61 0.00832573444871744 62-63 0.009118661539071482 64-65 0.0071363438131863775 66-67 0.005550489632478294 68-69 0.011893906355310629 70-71 0.003171708361416168 72-73 0.012290369900487649 74-75 0.006739880268009357 76-77 0.004757562542124252 78-79 0.016255005352257858 80-81 0.004757562542124252 82-83 0.013479760536018714 84-85 0.023787812710621258 86-87 0.01665146889743488 88-89 0.016255005352257858 90-91 0.03132062006898466 92-93 0.025373666891329343 94-95 0.030131229433453595 96-97 0.015065614716726797 98-99 0.0170479324426119 100 0.015065614716726797 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 126115.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.79978590968561 #Duplication Level Percentage of deduplicated Percentage of total 1 77.15158813104921 53.85164334139476 2 15.927886581542236 22.235261467708046 3 4.158903985095652 8.708718233358443 4 1.2496023992366065 3.488879197557784 5 0.44417685281955743 1.550172461642152 6 0.23515245149270686 0.9848154462197201 7 0.13177625301040577 0.643856797367482 8 0.12155223338028809 0.67874558934306 9 0.07724814831644476 0.48527137929667363 >10 0.4725769073476621 5.771716290687071 >50 0.022720043622483754 1.0720374261586647 >100 0.006816013086745126 0.528882369266146 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACCTGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 128 0.10149466756531737 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.96463545177021E-4 0.0 12-13 0.0 0.0 0.0 7.92927090354042E-4 0.0 14-15 0.0 0.0 0.0 7.92927090354042E-4 0.0 16-17 0.0 0.0 0.0 7.92927090354042E-4 0.0 18-19 0.0 0.0 0.0 7.92927090354042E-4 0.0 20-21 0.0 0.0 0.0 7.92927090354042E-4 0.0 22-23 0.0 0.0 0.0 7.92927090354042E-4 0.0 24-25 0.0 0.0 0.0 0.001189390635531063 0.0 26-27 0.0 0.0 0.0 0.001585854180708084 0.0 28-29 0.0 0.0 0.0 0.00396463545177021 0.0 30-31 0.0 0.0 0.0 0.006739880268009356 0.0 32-33 0.0 0.0 0.0 0.010308052174602546 0.0 34-35 0.0 0.0 0.0 0.013876224081195734 0.0 36-37 0.0 0.0 0.0 0.021409031439559134 0.0 38-39 0.0 0.0 0.0 0.04004281806287912 0.0 40-41 0.0 0.0 0.0 0.07810331839987314 0.0 42-43 0.0 0.0 0.0 0.1003052769297863 0.0 44-45 0.0 0.0 0.0 0.12924711572770883 0.0 46-47 0.0 0.0 0.0 0.17047932442611902 0.0 48-49 0.0 0.0 0.0 0.2172620227570075 0.0 50-51 0.0 0.0 0.0 0.25413313245847047 0.0 52-53 0.0 0.0 0.0 0.29655473179241165 3.96463545177021E-4 54-55 0.0 0.0 0.0 0.34968084684613254 7.92927090354042E-4 56-57 0.0 0.0 0.0 0.40915037862268566 7.92927090354042E-4 58-59 0.0 0.0 0.0 0.48130674384490346 7.92927090354042E-4 60-61 0.0 0.0 0.0 0.5415692027118106 7.92927090354042E-4 62-63 0.0 0.0 0.0 0.6034175157594259 7.92927090354042E-4 64-65 0.0 0.0 0.0 0.6640764381715101 7.92927090354042E-4 66-67 0.0 0.0 0.0 0.7512984181104547 7.92927090354042E-4 68-69 0.0 0.0 0.0 0.8678587003924989 7.92927090354042E-4 70-71 0.0 0.0 0.0 1.0018633786623319 7.92927090354042E-4 72-73 0.0 0.0 0.0 1.114062561947429 7.92927090354042E-4 74-75 0.0 0.0 0.0 1.210006739880268 7.92927090354042E-4 76-77 0.0 0.0 0.0 1.3095190897197002 7.92927090354042E-4 78-79 0.0 0.0 0.0 1.4423343773540023 7.92927090354042E-4 80-81 0.0 0.0 0.0 1.583078935891845 7.92927090354042E-4 82-83 0.0 0.0 0.0 1.7071720255322522 7.92927090354042E-4 84-85 0.0 0.0 0.0 1.8245252349046506 7.92927090354042E-4 86-87 0.0 0.0 0.0 1.926416366015145 7.92927090354042E-4 88 0.0 0.0 0.0 2.0148277365896208 7.92927090354042E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACGCA 25 5.557262E-5 75.15337 4 GTACCTG 405 0.0 70.97136 1 ACCTGGG 405 0.0 69.58646 3 TACCTGG 425 0.0 67.44373 2 CCTGGGG 310 0.0 56.061985 4 GGGCCTA 35 2.9364522E-4 53.680977 7 ATAACGC 35 2.9364522E-4 53.680977 3 TTACTCT 40 5.674727E-4 46.970856 4 TATAGCT 55 5.2755495E-5 42.70078 4 TATACAG 55 5.2755495E-5 42.70078 5 GTGTATA 45 9.978749E-4 41.884735 1 ATACTCC 50 0.0017014635 37.576687 6 CTGGGGG 180 0.0 36.532887 5 GAGTACC 65 1.3853492E-4 36.246407 1 TGGGGGC 80 1.15540115E-5 35.22814 6 ACATGGG 595 0.0 34.734753 3 GCATAGG 70 2.1415918E-4 33.657375 1 GTACATG 645 0.0 33.605194 1 TACATGG 630 0.0 33.56392 2 CCTGGGA 225 0.0 33.401497 4 >>END_MODULE