FastQCFastQC Report
Mon 6 Jun 2016
ERR1211642_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211642_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5304059
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201350.37961493263932394No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177060.3338198161068721No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118090.22264081149926876No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87440.164854878122585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96550.047.913481
TACCTGG62750.046.4828262
GTACCTG63350.046.449281
ACCTGGG71900.039.53633
GTATCAA159600.035.9582631
TCAACGC190700.029.4184024
CAACGCA192500.029.0681785
ATCAACG192950.029.0512733
GTACATG182450.029.025311
AACGCAG200850.027.9240996
TATCAAC203750.027.6151962
TACATGG187800.027.5557142
TAACGCA6000.027.4083084
TATAACG6100.026.9931012
ACATGGG186000.026.4991593
CCTGGGG70050.026.0248994
CATGGGG109050.025.5071224
ACGCAGA223850.024.9083167
CGCAGAG226550.024.3428368
ATGGGGG70600.022.3599765
Produced by FastQC (version 0.11.5)