FastQCFastQC Report
Mon 6 Jun 2016
ERR1211642_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211642_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5304059
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230450.4344785757473663No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208700.3934722445583656No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145460.2742428015977952No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90290.17022812151976438No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG60600.048.6560822
GGTATCA112550.046.914821
GTACCTG63800.046.701831
ACCTGGG73300.039.234693
GTATCAA181150.034.171491
TATAACG5800.034.053642
TAACGCA6300.032.8197254
TCAACGC223700.027.014554
ATCAACG225100.026.9509163
CAACGCA228150.026.5079865
TATCAAC235750.025.9717672
AACGCAG235450.025.8472486
GTACATG192400.025.5574051
CCTGGGG67600.025.0947744
TACATGG198250.024.527292
ATAACGC8950.024.1522483
ACATGGG190800.023.893
CATGGGG107400.023.1459054
ACGCAGA264250.022.9232947
CGCAGAG271000.022.2829658
Produced by FastQC (version 0.11.5)