FastQCFastQC Report
Mon 6 Jun 2016
ERR1211641_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211641_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8021768
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209220.26081532151017084No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183940.2293010717836766No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117260.1461772516981294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117200.046.544431
GTATCAA194600.035.1791841
TCAACGC226750.029.3015844
CAACGCA228450.029.080825
ATCAACG229650.028.8692863
AACGCAG238150.027.912416
GTACATG236700.027.8465161
TATCAAC249950.026.7622832
TACATGG241300.026.688422
ACATGGG241750.025.7528253
ACGCAGA273200.024.2457357
GTACCTG69050.023.829981
TACCTGG67250.023.5729732
CGCAGAG282200.023.2401498
CATGGGG147000.022.4073074
GCAGAGT315600.020.1402979
GAGTACT195400.019.84972212-13
ATGGGGG86050.019.0556135
AGAGTAC281500.018.83490410-11
CAGAGTA304950.018.60367610-11
Produced by FastQC (version 0.11.5)