FastQCFastQC Report
Mon 6 Jun 2016
ERR1211641_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211641_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8021768
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242890.30278861218624126No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218250.2720721915667469No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144560.1802096495435919No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA133300.044.8100741
GTATCAA216300.033.8270721
TCAACGC262300.027.3897274
GTACATG254400.027.1487431
ATCAACG265300.027.0277143
CAACGCA266800.027.0156425
TACATGG260350.026.0442072
AACGCAG277550.025.970746
TATCAAC281950.025.6333432
ACATGGG256200.024.9615213
CATGGGG155900.023.7497274
TACCTGG66550.023.5994512
GTACCTG69050.023.4868051
ACGCAGA319200.022.4642437
CGCAGAG327000.021.8421788
GAGTACT229600.019.68764912-13
GCAGAGT367300.019.176899
ATGGGGG89250.018.900015
AGAGTAC330700.018.83413510-11
ACCTGGG84050.017.9456563
Produced by FastQC (version 0.11.5)