FastQCFastQC Report
Mon 6 Jun 2016
ERR1211640_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211640_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3696678
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146860.39727560799182404No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124420.3365724577580195No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84160.22766386469148786No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63830.17266854186380312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG48050.053.7726022
GTACCTG49300.052.8344881
GGTATCA75050.045.5840261
ACCTGGG56050.044.519293
TATAACG4850.043.6669542
TAACGCA5050.039.0829434
GTATCAA124550.036.4465751
CCTGGGG48250.032.5295834
ATAACGC6550.030.8500963
TCAACGC147100.030.0931434
ATCAACG147650.029.9810473
CAACGCA149000.029.6119845
TATCAAC154700.028.9315912
AACGCAG154700.028.7592376
GTACATG118500.025.60461
ACGCAGA172550.025.53917
CGCAGAG174850.025.0973768
GTATAAC12600.025.0916751
TACATGG118150.024.8162672
ACATGGG115550.024.0351523
Produced by FastQC (version 0.11.5)