FastQCFastQC Report
Mon 6 Jun 2016
ERR1211640_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211640_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3696678
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160100.4330915486823575No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137680.37244250107799487No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100520.27191981557495676No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61710.1669336631429624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG47400.051.304592
GTACCTG51650.048.5746771
GGTATCA73150.045.4511
ACCTGGG54250.043.999083
TATAACG5100.038.7367442
GTATCAA122650.037.2200051
TAACGCA5950.033.9571464
CCTGGGG47500.032.8415874
TCAACGC156550.028.3634074
ATAACGC7650.028.2537483
ATCAACG158100.028.1447753
CAACGCA158500.028.1030125
TATCAAC161250.027.7122
AACGCAG165250.027.1840236
TATCACG4850.026.185812
GTACATG122050.025.8884241
TACATGG125500.024.5494382
ACATGGG120000.024.5116623
ACGCAGA185150.024.084627
CATGGGG70450.023.9438044
Produced by FastQC (version 0.11.5)