FastQCFastQC Report
Mon 6 Jun 2016
ERR1211639_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211639_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11979983
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350780.29280508995713933No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285810.23857295957765548No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187130.1562022249948101No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA208350.042.3101771
GTATCAA361900.035.345171
ATCAACG413300.029.5483343
TCAACGC415400.029.43244
CAACGCA418100.029.239285
GTACATG479850.028.8392281
AACGCAG435250.028.2883726
ACATGGG468950.027.4847263
TACATGG490100.027.4465472
TATCAAC458300.026.8665642
CATGGGG208550.026.2933274
ACGCAGA490000.024.9647057
CGCAGAG495800.024.5126118
TACCTGG81450.024.203672
GTACCTG88400.023.6897071
ATGGGGG132350.021.5833665
GCAGAGT551000.021.4940039
GAGTACT325400.020.1639512-13
CATGGGA243150.019.343934
AGAGTAC518400.018.87329710-11
Produced by FastQC (version 0.11.5)