FastQCFastQC Report
Mon 6 Jun 2016
ERR1211639_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211639_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11979983
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT394170.3290238391824095No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT325690.27186182150675836No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230690.1925628775933989No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA232750.038.4289171
GTATCAA395700.035.0555761
TCAACGC467300.028.8791144
ATCAACG467700.028.8448493
CAACGCA473300.028.5128975
AACGCAG489050.027.6243766
GTACATG496550.027.59391
TATCAAC503300.026.9276052
ACATGGG483500.026.5805113
TACATGG508350.026.4935952
CATGGGG213300.025.9507354
ACGCAGA555350.024.2163667
CGCAGAG561000.023.913848
TACCTGG82850.022.8172992
ATGGGGG128150.022.6968425
GTACCTG86700.022.6232361
GCAGAGT625450.021.036239
TAACGCA12450.020.3807264
GAGTACT367550.019.68163712-13
AGAGTAC585700.019.37921110-11
Produced by FastQC (version 0.11.5)