FastQCFastQC Report
Mon 6 Jun 2016
ERR1211638_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211638_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3601261
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA274080.7610667485639058No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217780.6047326200461449No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176150.4891342227069907No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138070.3833934835603418No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128510.35684722656869355No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG124220.3449347325839477No Hit
GTACCTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA103580.28762147481118416No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58160.16149898604960874No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG52650.14619878981279058No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG49540.13756292587513097No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC43770.12154076030590395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG80750.075.6295552
ACCTGGG82750.071.2586443
GTACCTG89450.068.801741
TATAACG5300.064.804682
CCTGGGG35650.053.7727744
TAACGCA6600.053.3923574
TATCACG3900.044.6371542
CCTGGGA68150.041.1595154
ATCACGC4350.041.044613
GTACCGG1051.1041266E-940.422441
GTATAAC11450.035.421121
GTACCCG4200.034.808211
TACCCGG4150.034.012032
ATAACGC11100.032.5933883
TCGCGTA902.5766694E-531.366652
ACAACGC2900.030.7834573
CTGGGGG25700.030.7102725
GTACACG4450.029.6733991
CCTGGGT22000.029.472584
CTGGGAA50300.029.2337065
Produced by FastQC (version 0.11.5)