FastQCFastQC Report
Mon 6 Jun 2016
ERR1211638_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211638_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3601261
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA355110.9860712678142461No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247690.6877868613244082No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208350.5785473477207012No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156220.4337924965727283No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG149910.41627085623619065No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147920.4107450140381383No Hit
GTACCTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA131440.3649832655839163No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG68740.19087758426839932No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG68700.19076651206341333No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63770.17707686279889182No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC60290.16741358096511194No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42300.11745885677266935No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT40890.1135435615469137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG82850.076.2271042
ACCTGGG84050.072.681523
GTACCTG92250.068.782841
TATAACG7900.066.6679152
TAACGCA8400.063.2137344
CCTGGGG35000.059.8796234
TATCACG3550.045.037732
CCTGGGA68250.042.894114
GTATAAC13350.042.3741841
ATCACGC4150.038.498973
ATAACGC14200.038.3873333
ACCGTAT1700.035.9335948
CTGGGGG23750.035.4156845
CCTGGGT16200.034.2278254
ACAACGC3050.033.8954283
GTACACG3650.032.2885481
GTACCGT2500.031.9532596
CGCGAGT753.2197483E-431.4275191
CTGGGAA55000.030.757155
TCAGTCG23350.029.3813848
Produced by FastQC (version 0.11.5)