##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211638_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3601261 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.9936666628717 33.0 31.0 34.0 30.0 34.0 2 32.23220005436984 33.0 31.0 34.0 30.0 34.0 3 32.31487526174859 34.0 31.0 34.0 30.0 34.0 4 35.98318866641435 37.0 35.0 37.0 35.0 37.0 5 35.90303396504724 37.0 35.0 37.0 35.0 37.0 6 35.95550919525133 37.0 35.0 37.0 35.0 37.0 7 35.87245745309768 37.0 35.0 37.0 35.0 37.0 8 35.85353158240961 37.0 35.0 37.0 35.0 37.0 9 37.46086523581601 39.0 37.0 39.0 34.0 39.0 10-11 37.41195042514275 39.0 37.0 39.0 34.0 39.0 12-13 37.33328020379528 39.0 37.0 39.0 34.0 39.0 14-15 38.49896091952236 40.0 38.0 41.0 34.0 41.0 16-17 38.43603046266294 40.0 38.0 41.0 34.0 41.0 18-19 38.47189359504907 40.0 38.0 41.0 34.0 41.0 20-21 38.41726828463696 40.0 38.0 41.0 34.0 41.0 22-23 38.31467255497449 40.0 38.0 41.0 34.0 41.0 24-25 38.225419790456726 40.0 37.0 41.0 34.0 41.0 26-27 37.98548633381474 40.0 37.0 41.0 33.0 41.0 28-29 37.9899107562601 40.0 37.0 41.0 33.0 41.0 30-31 37.79626969553165 40.0 37.0 41.0 33.0 41.0 32-33 37.45459687592762 39.5 36.0 41.0 32.5 41.0 34-35 37.28716260776433 39.0 36.0 41.0 32.0 41.0 36-37 37.174928726354466 40.0 36.0 41.0 31.0 41.0 38-39 37.07598907715936 40.0 36.0 41.0 31.0 41.0 40-41 36.929006256419626 39.0 35.5 41.0 31.0 41.0 42-43 36.75027969369618 39.0 35.0 41.0 30.0 41.0 44-45 36.50958289332542 39.0 35.0 41.0 30.0 41.0 46-47 36.179996673387464 39.0 35.0 41.0 28.5 41.0 48-49 35.904727121972 39.0 35.0 40.5 27.0 41.0 50-51 35.71131611954813 39.0 35.0 40.0 26.5 41.0 52-53 35.56408005418102 39.0 35.0 40.0 26.0 41.0 54-55 35.399952822080934 39.0 34.5 40.0 25.0 41.0 56-57 35.198782454257 38.0 34.0 40.0 25.0 41.0 58-59 34.99014706237621 38.0 34.0 40.0 24.0 41.0 60-61 34.67898425023901 38.0 34.0 40.0 23.0 41.0 62-63 34.29285866811653 38.0 33.0 40.0 22.0 41.0 64-65 34.052445934910025 37.0 33.0 40.0 22.0 41.0 66-67 33.687467667575326 36.5 32.0 39.0 22.0 41.0 68-69 33.29774681701771 36.0 32.0 39.0 21.0 40.0 70-71 32.915266902343376 36.0 31.0 39.0 20.0 40.0 72-73 32.45224506082731 35.0 31.0 38.0 20.0 40.0 74-75 32.02661317799515 35.0 30.5 37.0 19.0 39.0 76-77 30.482838233607616 33.5 28.5 35.5 18.0 38.5 78-79 31.30495123791361 34.0 30.0 36.0 19.0 38.5 80-81 31.243405573769856 34.0 30.0 36.0 18.5 37.5 82-83 30.98318477888717 34.0 30.0 35.5 18.0 37.0 84-85 30.646881467352685 34.0 30.0 35.0 18.0 37.0 86-87 30.31638598257666 34.0 29.0 35.0 17.0 36.0 88-89 30.038012379552608 34.0 29.0 35.0 15.5 36.0 90-91 29.733410324883423 34.0 29.0 35.0 14.0 35.5 92-93 29.456414572562224 34.0 29.0 35.0 12.0 35.0 94-95 29.304011011698403 34.0 28.5 35.0 11.0 35.0 96-97 29.062744827436834 33.5 27.0 35.0 10.5 35.0 98-99 28.795559388780763 33.0 26.5 35.0 10.0 35.0 100 27.138343208115156 31.0 24.0 34.0 8.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2199641148535214 1101 2 0.15447340193502868 1101 3 0.1312187209865101 1101 4 0.14318119740108415 1101 5 0.11188709586865286 1101 6 0.1383729302693837 1101 7 0.13503977520175425 1101 8 0.1500691378825607 1101 9 0.18199195375311916 1101 10-11 0.1423234606996644 1101 12-13 0.1260030651900763 1101 14-15 0.10361953546330227 1101 16-17 0.08375904099471398 1101 18-19 0.18310005048483902 1101 20-21 0.13387897389366543 1101 22-23 -0.010403873841895006 1101 24-25 0.043260802167303325 1101 26-27 0.09777327638789757 1101 28-29 0.10281148467791468 1101 30-31 0.19218179872896712 1101 32-33 0.2171999414597252 1101 34-35 0.2321457248838925 1101 36-37 0.16384250508166787 1101 38-39 0.1548413144403753 1101 40-41 0.16872206563044045 1101 42-43 0.1823199145417007 1101 44-45 0.22693042558851317 1101 46-47 0.06346082448244772 1101 48-49 0.04279959637145936 1101 50-51 0.041910706312727086 1101 52-53 -0.03810966866294052 1101 54-55 -0.09957879365863676 1101 56-57 -0.20997372310717566 1101 58-59 -0.24687111918212423 1101 60-61 -0.214812423681785 1101 62-63 -0.3526870758049725 1101 64-65 -0.1927763778339795 1101 66-67 -0.2638333621071638 1101 68-69 -0.3498785711054211 1101 70-71 -0.3351004999905527 1101 72-73 -0.41454451419150473 1101 74-75 -0.477688194742079 1101 76-77 -0.3319241156678707 1101 78-79 -0.3019666212914096 1101 80-81 -0.3448112709889024 1101 82-83 -0.41728942632481747 1101 84-85 -0.3411803988035942 1101 86-87 -0.357166936340807 1101 88-89 -0.49675578644961504 1101 90-91 -0.6121770259310182 1101 92-93 -0.685543868946997 1101 94-95 -0.6467465942938126 1101 96-97 -0.9150727293464733 1101 98-99 -0.9018742236782771 1101 100 -0.9955104801000978 1102 1 0.2571131582565833 1102 2 0.14824150445581807 1102 3 0.11146571806987993 1102 4 0.1023394293940143 1102 5 0.12270641390536952 1102 6 0.11509202971677723 1102 7 0.1450464947057668 1102 8 0.08920926893501502 1102 9 0.12017804798294662 1102 10-11 0.2434265924436687 1102 12-13 0.1822717603293995 1102 14-15 0.1880755385152142 1102 16-17 0.17399232409506737 1102 18-19 0.15983965453348503 1102 20-21 0.0794253112898815 1102 22-23 0.05138198131770366 1102 24-25 0.14506638441264386 1102 26-27 0.08040540503573368 1102 28-29 0.2824379153380647 1102 30-31 0.30110034418075315 1102 32-33 0.29757023480431855 1102 34-35 0.30527927676440925 1102 36-37 0.3362798012999093 1102 38-39 0.37108220707875716 1102 40-41 0.2776767213048501 1102 42-43 0.28990032734554205 1102 44-45 0.2635122145316089 1102 46-47 0.30114802872628843 1102 48-49 0.28547044592410487 1102 50-51 0.3378963636799739 1102 52-53 0.29582751661711626 1102 54-55 0.25039334536872815 1102 56-57 0.2512455176204824 1102 58-59 0.2419836225879095 1102 60-61 0.22800613011407478 1102 62-63 0.21793756625343264 1102 64-65 0.10828027681385777 1102 66-67 0.00931562155994925 1102 68-69 0.01448961536176796 1102 70-71 0.07999508226743757 1102 72-73 -0.04008280935914854 1102 74-75 -0.10987586739161159 1102 76-77 0.045510716837586784 1102 78-79 0.004159292289319438 1102 80-81 0.12238594993043606 1102 82-83 0.0313514070201073 1102 84-85 0.06344163857783158 1102 86-87 0.211965926837955 1102 88-89 0.030832305110106262 1102 90-91 -0.05460536017671913 1102 92-93 -0.10913416378340912 1102 94-95 -0.04448295743021902 1102 96-97 -0.07380505035039064 1102 98-99 -0.2860689372681122 1102 100 -0.23496028989325168 1103 1 0.24624484464264995 1103 2 0.13727677398699 1103 3 0.12329152167672675 1103 4 0.13756973364139213 1103 5 0.14120386469286217 1103 6 0.11420616147992746 1103 7 0.11190473613602592 1103 8 0.1616459623650357 1103 9 0.22140177438998165 1103 10-11 0.10130457875615662 1103 12-13 0.13375354767681102 1103 14-15 0.1927178642248606 1103 16-17 0.2132544802952836 1103 18-19 0.17712932300689488 1103 20-21 0.06735147992971946 1103 22-23 0.06123991384556149 1103 24-25 0.07461817346730726 1103 26-27 0.026354737978486753 1103 28-29 0.05299915508686581 1103 30-31 0.23822427238765442 1103 32-33 0.2845743788867168 1103 34-35 0.07184148160294512 1103 36-37 0.15059306764501912 1103 38-39 0.1782522587753803 1103 40-41 0.10322170683003407 1103 42-43 0.10238850553371748 1103 44-45 0.09418763609435388 1103 46-47 0.17345764535568264 1103 48-49 0.32291668738630364 1103 50-51 0.2962635887660383 1103 52-53 0.19168046341097522 1103 54-55 0.11126835289169179 1103 56-57 0.048762253928366306 1103 58-59 0.0438490584350717 1103 60-61 0.09122983993987788 1103 62-63 0.15810353835026802 1103 64-65 0.08282580491415104 1103 66-67 0.13027835767377383 1103 68-69 0.0778885336506292 1103 70-71 0.003956612863511566 1103 72-73 -0.1323093309337935 1103 74-75 -0.17863958529922996 1103 76-77 -0.05197474540446834 1103 78-79 0.011179874831359626 1103 80-81 0.019391715289884814 1103 82-83 -0.06866280986008988 1103 84-85 -0.08843283251123424 1103 86-87 -0.05292191794442047 1103 88-89 -0.11762067263540743 1103 90-91 -0.014066486681773682 1103 92-93 -0.02903094861906297 1103 94-95 -0.1665888781006437 1103 96-97 -0.27487134707110883 1103 98-99 -0.1996321864178654 1103 100 -0.5527892402514674 1104 1 0.3397962914029584 1104 2 0.19563502571959646 1104 3 0.20207669678165274 1104 4 0.14321177839504173 1104 5 0.11172867370923001 1104 6 0.11378478650497925 1104 7 0.16203924610326936 1104 8 0.1980950125561307 1104 9 0.24283623036640734 1104 10-11 0.20024748884080168 1104 12-13 0.2639944802853762 1104 14-15 0.2689572162369771 1104 16-17 0.2150751295374107 1104 18-19 0.22154284262732915 1104 20-21 0.12022400837175695 1104 22-23 0.2215350738834445 1104 24-25 0.15488359758699488 1104 26-27 0.18379166171298777 1104 28-29 0.15047996368882366 1104 30-31 0.2865830807977261 1104 32-33 0.2165454451353952 1104 34-35 0.23024489542172688 1104 36-37 0.2586571034708527 1104 38-39 0.18179826357322781 1104 40-41 0.19354342888227904 1104 42-43 0.11394534402535328 1104 44-45 0.13450413977397346 1104 46-47 0.2727814621844402 1104 48-49 0.2052859541348866 1104 50-51 0.14877472863243923 1104 52-53 0.227830615325729 1104 54-55 0.1702005873268746 1104 56-57 0.2735840271689014 1104 58-59 0.06162112037834788 1104 60-61 -0.07078646420112733 1104 62-63 0.017850374917657064 1104 64-65 0.11734788582810296 1104 66-67 0.11141947295983812 1104 68-69 -0.00209483800310295 1104 70-71 0.1318354948560838 1104 72-73 0.1506455344693194 1104 74-75 0.10502452700658438 1104 76-77 0.06499168862921678 1104 78-79 0.13978714669295655 1104 80-81 0.14716511953795575 1104 82-83 0.1779022719757073 1104 84-85 0.047070501542638254 1104 86-87 0.16965763964937608 1104 88-89 0.23835117097084435 1104 90-91 0.16481714452759988 1104 92-93 0.08692301392519397 1104 94-95 0.01311468464276544 1104 96-97 0.0020937224519279596 1104 98-99 -0.03189408792546544 1104 100 -0.11762275349039797 1105 1 0.29020763990071785 1105 2 0.22393322130748317 1105 3 0.18665594096692928 1105 4 0.12944405625932376 1105 5 0.1542390477815232 1105 6 0.11455803858774516 1105 7 0.08501521197973716 1105 8 0.08422942023453572 1105 9 0.09163881580726496 1105 10-11 0.17589876082601563 1105 12-13 0.13230048905214176 1105 14-15 0.2143792549714192 1105 16-17 0.2781488195021353 1105 18-19 0.23341989417649955 1105 20-21 0.14849370731479183 1105 22-23 0.1441687376053764 1105 24-25 0.1019651378637505 1105 26-27 0.15441314271976125 1105 28-29 0.125768198310638 1105 30-31 0.27743873954656095 1105 32-33 0.26915671939730146 1105 34-35 0.18345667655106013 1105 36-37 0.22622483988153164 1105 38-39 0.18601964772945934 1105 40-41 0.19128443250291838 1105 42-43 0.2265892711049915 1105 44-45 0.15749819283997368 1105 46-47 0.20939141761097346 1105 48-49 0.32168854681975034 1105 50-51 0.2435173221299678 1105 52-53 0.2276505043554593 1105 54-55 0.2110917508407013 1105 56-57 0.22110621916886686 1105 58-59 0.14334432352602278 1105 60-61 0.16457851123849565 1105 62-63 0.14199975577573554 1105 64-65 0.1258540011548206 1105 66-67 0.10528732979452116 1105 68-69 0.11567528926467219 1105 70-71 0.034734528161628475 1105 72-73 0.12898633425334083 1105 74-75 0.023692249127080345 1105 76-77 0.05266139204648468 1105 78-79 0.15136271971901039 1105 80-81 0.05580152806889771 1105 82-83 0.11194836638307848 1105 84-85 0.10869013974101804 1105 86-87 0.07384233199746504 1105 88-89 0.014010440278823921 1105 90-91 0.10390113376568522 1105 92-93 0.263966048348383 1105 94-95 0.16097997015936372 1105 96-97 0.19235748800543107 1105 98-99 0.17385800359130243 1105 100 0.02291592669110898 1106 1 0.2601326146689438 1106 2 0.17135240551778708 1106 3 0.11394722886054609 1106 4 0.05243749303402012 1106 5 0.05732251246238462 1106 6 0.0631280702416035 1106 7 0.06811111168359929 1106 8 0.05042833553524417 1106 9 0.03584087514669676 1106 10-11 0.08880386268410234 1106 12-13 0.10368926230776765 1106 14-15 0.14722983426440095 1106 16-17 0.1516349132682535 1106 18-19 0.08961088415139784 1106 20-21 0.11476607938585914 1106 22-23 0.0850545684392543 1106 24-25 0.19147106362364497 1106 26-27 0.14673361170454768 1106 28-29 0.20644500571781066 1106 30-31 0.3265016357387296 1106 32-33 0.3908778493299465 1106 34-35 0.3502475007256436 1106 36-37 0.2869042090101743 1106 38-39 0.2971400243262323 1106 40-41 0.32888230568109833 1106 42-43 0.3348300830046611 1106 44-45 0.33474085546480836 1106 46-47 0.4552544894470998 1106 48-49 0.38540773648556836 1106 50-51 0.4270466538408755 1106 52-53 0.49959502885370455 1106 54-55 0.33397336432199864 1106 56-57 0.38269384308918575 1106 58-59 0.4169777607513936 1106 60-61 0.36398302959334927 1106 62-63 0.387886250759621 1106 64-65 0.24251602766626945 1106 66-67 0.42121496649971846 1106 68-69 0.38305808161224064 1106 70-71 0.48283483675418637 1106 72-73 0.33518860588358734 1106 74-75 0.25888066611366867 1106 76-77 0.20042409599094313 1106 78-79 0.2879176698306729 1106 80-81 0.26062576254706116 1106 82-83 0.3293299282741451 1106 84-85 0.298790405638222 1106 86-87 0.3488419465532644 1106 88-89 0.3778783669329435 1106 90-91 0.2898156979824691 1106 92-93 0.34647097313932207 1106 94-95 0.4443297886447901 1106 96-97 0.3083799736342918 1106 98-99 0.36394248110092775 1106 100 0.2855402620963545 1107 1 0.1705446668558217 1107 2 0.11087322564075919 1107 3 0.07006905171252953 1107 4 0.05703082160137285 1107 5 0.08036688055749863 1107 6 0.07169182749522918 1107 7 0.07928788673235232 1107 8 0.07744715763603693 1107 9 0.08839127360940324 1107 10-11 0.12956523501647865 1107 12-13 0.18737170061498887 1107 14-15 0.09437329196400412 1107 16-17 0.16222245474368435 1107 18-19 0.1215728350758738 1107 20-21 0.12361449990907403 1107 22-23 0.1673022731289393 1107 24-25 0.15890000868549947 1107 26-27 0.24912749111125265 1107 28-29 0.19100252650748928 1107 30-31 0.22604517915567612 1107 32-33 0.09627046727473498 1107 34-35 0.1518888722851699 1107 36-37 0.18211193452027885 1107 38-39 0.1451571007557746 1107 40-41 0.06615794169619704 1107 42-43 0.19741266792133416 1107 44-45 0.1837347305388093 1107 46-47 0.09906725881415923 1107 48-49 0.021848096767762115 1107 50-51 -0.06758536316910124 1107 52-53 -0.009822462052007097 1107 54-55 0.026947652338137118 1107 56-57 0.08069524272933393 1107 58-59 -0.013368002475516505 1107 60-61 -0.06889762363841356 1107 62-63 -0.013210517040633363 1107 64-65 0.02870280746343923 1107 66-67 0.08129441752227251 1107 68-69 -0.0754012808123008 1107 70-71 0.060340056495640226 1107 72-73 0.015816104875057135 1107 74-75 0.05469765665021953 1107 76-77 -0.11704816150771435 1107 78-79 0.07776697797341114 1107 80-81 0.09263494876507039 1107 82-83 0.12544212109836295 1107 84-85 0.1144998293939885 1107 86-87 0.07403939212948174 1107 88-89 -0.12087027104534442 1107 90-91 0.07529603422398523 1107 92-93 0.0976760805816852 1107 94-95 -0.04449504643421065 1107 96-97 -0.05450150954514399 1107 98-99 0.111705428138265 1107 100 0.260384577379277 1108 1 0.1751117473762882 1108 2 0.10787757962588529 1108 3 0.06184976100772843 1108 4 0.05662298870444005 1108 5 -0.009332671503038625 1108 6 0.07078624408649148 1108 7 0.06924231095617728 1108 8 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0.10877644703648315 2316 70-71 -0.1511557715011307 2316 72-73 -0.452503497984992 2316 74-75 -0.054013110015400656 2316 76-77 0.004456069140562846 2316 78-79 -0.19002718679065467 2316 80-81 -0.10534682769023718 2316 82-83 -0.025374040400411246 2316 84-85 0.08973208260930932 2316 86-87 0.24124169924713357 2316 88-89 0.2614310372503219 2316 90-91 0.2766481186384162 2316 92-93 0.24913834639492904 2316 94-95 0.32698450218185826 2316 96-97 0.3586324319749856 2316 98-99 0.27197551413396326 2316 100 -0.09655506852838869 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 4.0 11 24.0 12 69.0 13 183.0 14 635.0 15 1759.0 16 4649.0 17 11073.0 18 19851.0 19 27052.0 20 31783.0 21 38143.0 22 45179.0 23 45321.0 24 42904.0 25 43835.0 26 48974.0 27 57043.0 28 67471.0 29 80740.0 30 94869.0 31 114091.0 32 138953.0 33 174118.0 34 229593.0 35 311404.0 36 438987.0 37 695782.0 38 735758.0 39 100987.0 40 27.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.23028356040065 19.776236157430862 12.541214527111672 21.452265755056818 2 14.886013065429138 22.947205539426253 41.08159543079594 21.08518596434867 3 16.567991636156133 33.74305616298944 29.71622077548827 19.97273142536616 4 10.869573064958137 18.824417344421775 38.90957165432782 31.39643793629227 5 9.827368226967922 39.498129676676065 34.89444912029132 15.780052976064693 6 25.279883151196742 37.879394152283865 22.685800843321804 14.154921853197584 7 25.725405628750597 32.87587320108151 25.603559419880977 15.795161750286912 8 27.362625950162528 33.24978771118502 23.010024919291375 16.377561419361072 9 29.264415992065 15.07574707859275 22.460771379802797 33.199065549539455 10-11 22.980797386022008 28.092057920371914 30.018261691863213 18.908883001742865 12-13 24.66637944834923 25.741087820633595 30.141553830733812 19.450978900283364 14-15 22.753005510154015 25.767172355346133 29.44817515978086 22.031646974718992 16-17 20.227437253159106 28.616938474538195 30.476927917113382 20.678696355189313 18-19 20.76923984735718 27.157496041269376 32.88117143715979 19.19209267421365 20-21 20.62868573932491 27.12108510165053 33.405818209485645 18.84441094953892 22-23 19.858766137750084 27.748238741929566 31.666269120732988 20.726725999587366 24-25 19.610908512323878 28.04385741549974 31.818313085333166 20.52692098684322 26-27 20.735654960140963 26.572900241540896 32.09143705101571 20.60000774730243 28-29 20.180590405166296 27.470740211285833 32.68978181644502 19.658887567102855 30-31 21.35847759339752 27.60419949722217 32.23337377363557 18.803949135744737 32-33 19.994004712923218 27.253814185532338 32.87705468401588 19.875126417528563 34-35 19.411212016740954 28.35494172004489 32.55771589283803 19.67613037037613 36-37 21.205358754418654 27.36664898742367 32.17285301329276 19.25513924486492 38-39 21.58172594104353 27.735873708068414 31.413061694067878 19.269338656820178 40-41 20.360655546502578 28.6765632905733 31.55972769982777 19.403053463096352 42-43 21.319702431883677 27.582107699173708 30.812274754227474 20.285915114715138 44-45 20.93537915355607 27.84801338429972 31.221261808216227 19.995345653927988 46-47 20.425460540228165 27.81854174190586 32.00308357461944 19.752914143246535 48-49 20.563526485128307 28.331947607915797 31.554189639439244 19.550336267516652 50-51 20.12730361703002 28.422407635939923 31.248535160046607 20.201753586983447 52-53 19.844579426691386 29.0448670029313 29.890378914185874 21.220174656191443 54-55 20.23780018514052 29.27712581488419 29.836740604854388 20.648333395120893 56-57 20.856901265302554 28.25101392207691 29.76859944767107 21.123485364949467 58-59 20.360353105501236 29.079623679950238 30.330585167680685 20.22943804686784 60-61 21.494338147154437 29.224374827391003 29.896621355459494 19.38466566999506 62-63 19.94959831951816 29.01355560542002 30.76713085052732 20.2697152245345 64-65 19.815831432122433 28.97212145144904 30.52959397744151 20.68245313898702 66-67 20.799770729206642 28.771179639331717 30.06983928272899 20.359210348732653 68-69 20.870815451478137 28.143755813078343 30.045807543330955 20.939621192112572 70-71 20.377078440452003 29.10490730976512 30.178563122594927 20.339451127187953 72-73 20.879863541321548 29.0385850688056 29.35698680042224 20.724564589450615 74-75 20.968186710832814 29.019413776594387 29.495829986992316 20.516569525580486 76-77 20.39009925762621 28.48031710456034 30.969151367914684 20.160432269898767 78-79 20.56740365931507 28.378348166086287 30.657813812047362 20.39643436255128 80-81 19.83093860468462 29.183925185215937 30.177121657473155 20.808014552626293 82-83 19.908610350772733 29.564927836975553 28.99436393004097 21.53209788221075 84-85 19.911221086408485 29.82334346043618 28.606188554647122 21.659246898508208 86-87 20.278583644524726 28.980333703819117 29.073399509758996 21.667683141897164 88-89 19.76368303421097 28.81631139738479 30.025710474918228 21.39429509348601 90-91 21.08751875255022 29.239937369745988 29.488411264223952 20.18413261347984 92-93 19.68782813590821 29.475081148214073 29.815019707076228 21.02207100880149 94-95 19.2101255696744 29.021420382382328 29.961467338204834 21.80698670973844 96-97 20.619261185251368 28.451245755795735 29.91169222296391 21.017800835988993 98-99 20.82021700354035 28.425700206838663 29.420011622957908 21.33407116666308 100 18.69334733856604 29.802511221174267 29.93190779542843 21.57223364483126 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3514.0 1 2643.5 2 5828.5 3 8178.5 4 4662.5 5 3144.5 6 4352.5 7 7406.5 8 10869.0 9 12094.0 10 10296.0 11 8409.5 12 8369.5 13 8829.0 14 9357.5 15 9915.5 16 10478.0 17 11032.5 18 11748.5 19 12381.5 20 13031.5 21 13934.0 22 15285.0 23 17168.5 24 18972.5 25 20860.5 26 23833.0 27 27304.0 28 30846.0 29 36220.0 30 44005.5 31 51852.0 32 62504.5 33 77146.0 34 91845.5 35 104539.0 36 120043.5 37 134965.5 38 137885.0 39 136315.0 40 130151.5 41 123610.0 42 122384.5 43 126248.0 44 138619.0 45 152747.5 46 153937.5 47 139361.5 48 129745.5 49 138776.0 50 183609.5 51 182371.5 52 122310.5 53 87900.5 54 69162.0 55 56176.5 56 47437.0 57 39568.0 58 31793.0 59 23963.5 60 16736.5 61 11734.5 62 8631.5 63 6337.5 64 4498.5 65 3205.0 66 2345.0 67 1783.5 68 1398.0 69 1069.0 70 822.5 71 620.0 72 487.0 73 392.5 74 302.0 75 226.0 76 151.5 77 95.0 78 57.0 79 48.0 80 36.5 81 24.0 82 20.5 83 16.0 84 10.5 85 9.5 86 6.0 87 3.0 88 4.0 89 2.0 90 0.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2521616733694115 2 0.08080502912729735 3 0.001277330357338721 4 9.441137423807939E-4 5 3.3321661495792725E-4 6 7.219693324088424E-4 7 0.0 8 9.718817936272879E-4 9 0.0 10-11 1.804923331022106E-4 12-13 0.0014300546391944377 14-15 1.388402562324697E-5 16-17 1.1107220498597575E-4 18-19 0.0012079102292224862 20-21 0.0013884025623246967 22-23 0.0 24-25 0.0 26-27 2.0826038434870452E-4 28-29 0.0020270677409940574 30-31 0.0018882274847615878 32-33 0.0027490370734028994 34-35 0.004206859763843831 36-37 0.0014161706135711906 38-39 0.0031239057652305675 40-41 0.006525492042926075 42-43 0.013106520188345136 44-45 0.009038500680733776 46-47 0.006817056581014261 48-49 0.0071919252728419305 50-51 0.005137089480601378 52-53 0.0035820786107977182 54-55 0.005900710889879961 56-57 0.004054135481988115 58-59 0.0019715316385010696 60-61 0.0076500981184090796 62-63 0.01080177193488614 64-65 0.007400185657190634 66-67 0.007747286297771809 68-69 0.015105819878092702 70-71 0.0042623958663368195 72-73 0.012884375778373185 74-75 0.008691400040152602 76-77 0.004928829096252673 78-79 0.017063467490970523 80-81 0.007261345400958164 82-83 0.014578226904409316 84-85 0.023783335892622055 86-87 0.016188773876705965 88-89 0.01634149815856168 90-91 0.030100567551199428 92-93 0.025116202352453765 94-95 0.02967016275687877 96-97 0.017091235542217018 98-99 0.017452220208421437 100 0.019493171975038744 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3601261.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.06001137530102 #Duplication Level Percentage of deduplicated Percentage of total 1 74.46798824650166 32.81060409236646 2 15.673786592768177 13.811744311428129 3 4.5952872882891445 6.074052305843878 4 1.8100213098651785 3.189982380087881 5 0.9198029623643318 2.026326449240402 6 0.5047013839174681 1.3342289229920286 7 0.32784178777308215 1.0111299029006715 8 0.23572599720046447 0.8308872094485313 9 0.17106224315772675 0.6783303941472555 >10 1.01664644009279 8.70880485215165 >50 0.12752234103704077 3.9548653573058354 >100 0.12983540288589393 11.735941323426937 >500 0.012802041444116643 3.8364394215934476 >1k 0.006132714464244921 4.396140417808289 >5k 3.0663572321224604E-4 0.8832406644771411 >10k+ 5.366125156214305E-4 4.7172819947814695 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 35511 0.9860712678142461 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24769 0.6877868613244082 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20835 0.5785473477207012 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15622 0.4337924965727283 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 14991 0.41627085623619065 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14792 0.4107450140381383 No Hit GTACCTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 13144 0.3649832655839163 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 6874 0.19087758426839932 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 6870 0.19076651206341333 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6377 0.17707686279889182 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 6029 0.16741358096511194 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4230 0.11745885677266935 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 4089 0.1135435615469137 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.7768051246493935E-5 0.0 3 0.0 0.0 0.0 2.7768051246493935E-5 0.0 4 0.0 0.0 0.0 5.553610249298787E-5 0.0 5 0.0 0.0 0.0 5.553610249298787E-5 0.0 6 0.0 0.0 0.0 5.553610249298787E-5 0.0 7 0.0 0.0 0.0 5.553610249298787E-5 0.0 8 0.0 0.0 0.0 5.553610249298787E-5 0.0 9 0.0 0.0 0.0 5.553610249298787E-5 0.0 10-11 0.0 0.0 0.0 5.553610249298787E-5 0.0 12-13 0.0 0.0 0.0 5.553610249298787E-5 0.0 14-15 0.0 0.0 0.0 5.553610249298787E-5 0.0 16-17 0.0 0.0 0.0 8.330415373948181E-5 0.0 18-19 0.0 0.0 0.0 1.249562306092227E-4 0.0 20-21 0.0 0.0 0.0 1.8049233310221056E-4 0.0 22-23 0.0 0.0 0.0 4.16520768697409E-4 0.0 24-25 0.0 0.0 0.0 9.02461665511053E-4 0.0 26-27 0.0 0.0 0.0 0.0014855907416874256 0.0 28-29 0.0 0.0 0.0 0.0036931508157836933 0.0 30-31 0.0 0.0 0.0 0.01039913519181198 0.0 32-33 0.0 0.0 0.0 0.024033248353840502 0.0 34-35 0.0 0.0 0.0 0.041999177510322075 0.0 36-37 0.0 0.0 0.0 0.06769850893895221 0.0 38-39 0.0 0.0 0.0 0.11357132959816019 0.0 40-41 0.0 0.0 0.0 0.1799369720772807 0.0 42-43 0.0 0.0 0.0 0.23419574421292988 0.0 44-45 0.0 0.0 0.0 0.2914673499088236 0.0 46-47 0.0 0.0 0.0 0.3533623361372586 0.0 48-49 0.0 0.0 0.0 0.42178281440861964 0.0 50-51 0.0 0.0 0.0 0.500060950872486 0.0 52-53 0.0 0.0 0.0 0.5807410237691741 0.0 54-55 0.0 0.0 0.0 0.6710566104483957 0.0 56-57 0.0 0.0 0.0 0.770466233910844 0.0 58-59 0.0 0.0 0.0 0.8783173449522265 0.0 60-61 0.0 0.0 0.0 0.982683565562174 0.0 62-63 0.0 0.0 0.0 1.0991983085924624 0.0 64-65 0.0 0.0 0.0 1.2161851084939415 0.0 66-67 0.0 0.0 0.0 1.3338105735740897 0.0 68-69 0.0 2.7768051246493935E-5 0.0 1.4782044400558583 0.0 70-71 0.0 2.7768051246493935E-5 0.0 1.6440630101511666 0.0 72-73 0.0 2.7768051246493935E-5 0.0 1.7869851699168708 0.0 74-75 2.7768051246493935E-5 2.7768051246493935E-5 0.0 1.932267614038527 0.0 76-77 2.7768051246493935E-5 2.7768051246493935E-5 0.0 2.0839228259212534 0.0 78-79 2.7768051246493935E-5 2.7768051246493935E-5 0.0 2.2505450174258406 0.0 80-81 2.7768051246493935E-5 2.7768051246493935E-5 0.0 2.413612898370876 0.0 82-83 2.7768051246493935E-5 2.7768051246493935E-5 0.0 2.5900927480679687 0.0 84-85 2.7768051246493935E-5 2.7768051246493935E-5 0.0 2.775972083111999 0.0 86-87 2.7768051246493935E-5 2.7768051246493935E-5 0.0 2.959824350415035 0.0 88 2.7768051246493935E-5 2.7768051246493935E-5 0.0 3.1067451095602348 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 8285 0.0 76.227104 2 ACCTGGG 8405 0.0 72.68152 3 GTACCTG 9225 0.0 68.78284 1 TATAACG 790 0.0 66.667915 2 TAACGCA 840 0.0 63.213734 4 CCTGGGG 3500 0.0 59.879623 4 TATCACG 355 0.0 45.03773 2 CCTGGGA 6825 0.0 42.89411 4 GTATAAC 1335 0.0 42.374184 1 ATCACGC 415 0.0 38.49897 3 ATAACGC 1420 0.0 38.387333 3 ACCGTAT 170 0.0 35.933594 8 CTGGGGG 2375 0.0 35.415684 5 CCTGGGT 1620 0.0 34.227825 4 ACAACGC 305 0.0 33.895428 3 GTACACG 365 0.0 32.288548 1 GTACCGT 250 0.0 31.953259 6 CGCGAGT 75 3.2197483E-4 31.427519 1 CTGGGAA 5500 0.0 30.75715 5 TCAGTCG 2335 0.0 29.381384 8 >>END_MODULE