FastQCFastQC Report
Mon 6 Jun 2016
ERR1211637_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211637_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7081080
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229090.32352409519451836No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200430.28305004321374705No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128340.18124353912115101No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119300.045.3763961
GTATCAA197200.037.0162661
TCAACGC241650.029.2632924
CAACGCA243300.029.061765
ATCAACG245800.028.8279933
GTACATG235650.028.2950531
AACGCAG252000.028.2414786
TATCAAC255350.027.9860632
TACATGG245050.026.5897942
ACATGGG249550.025.3067683
GTACCTG60450.024.7281441
ACGCAGA287150.024.5722927
TACCTGG58800.024.3229452
CGCAGAG297750.023.5254558
CATGGGG155900.023.1768114
GCAGAGT327450.020.5879449
AGAGTAC293500.019.64143210-11
GTATAGG23100.019.3927841
GAGTACT200450.019.25598712-13
ATGGGGG88400.018.9733245
Produced by FastQC (version 0.11.5)