FastQCFastQC Report
Mon 6 Jun 2016
ERR1211637_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211637_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7081080
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262270.37038135425669527No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239910.33880425019912214No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160710.2269569048789168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA139850.045.6493451
GTATCAA221850.035.0460931
TACCTGG56850.028.1234282
TCAACGC274650.027.7157734
CAACGCA276400.027.4719035
ATCAACG279400.027.2617863
GTACATG255600.026.6738571
TATCAAC290150.026.5791512
AACGCAG287750.026.5686746
GTACCTG63350.025.9006711
TACATGG264900.025.2960512
ACATGGG265000.024.5230223
CATGGGG157950.023.650434
ACGCAGA330700.022.9757927
CGCAGAG343650.022.0279358
TATAACG7350.021.112832
GAGTACT224050.020.42697312-13
ACCTGGG76100.020.129393
TAACGCA7300.019.954024
GCAGAGT375550.019.7813369
Produced by FastQC (version 0.11.5)