FastQCFastQC Report
Mon 6 Jun 2016
ERR1211636_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211636_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5738695
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185540.3233139241587155No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162500.283165423497851No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106620.18579136894363613No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62190.10836958576819293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91100.049.597121
TACCTGG62100.041.1348152
GTACCTG62900.040.865351
GTATCAA151350.035.992241
ACCTGGG71850.034.5288433
TCAACGC179150.029.5060164
CAACGCA182350.028.9851935
ATCAACG182400.028.9545173
GTACATG164250.027.9680981
AACGCAG191450.027.849476
TATCAAC192350.027.7590732
TACATGG167700.026.7899322
ACATGGG167400.025.7949643
ACGCAGA215100.024.6127417
CGCAGAG218300.024.0380158
CATGGGG107400.023.4495964
CCTGGGG70850.022.1503944
TATAACG5750.022.0900782
TAACGCA6900.021.7909284
GCAGAGT241850.020.8615239
Produced by FastQC (version 0.11.5)