FastQCFastQC Report
Mon 6 Jun 2016
ERR1211636_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211636_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5738695
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209990.36591942941731526No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188330.3281756566606171No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130170.22682857339517085No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65880.11479961907715952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104650.046.6387831
GTACCTG61700.040.3547671
TACCTGG61250.039.466062
GTATCAA171300.035.1543921
ACCTGGG76250.030.691853
TCAACGC208600.028.00164
ATCAACG210050.027.9206493
CAACGCA211200.027.5676425
TAACGCA6250.027.0675224
AACGCAG218750.026.7889566
TATCAAC219650.026.7850742
GTACATG182850.025.6610371
TATAACG7300.025.125142
TACATGG185350.024.5865692
ACATGGG184350.023.7068253
ACGCAGA252050.023.1191797
CGCAGAG256000.022.6706718
CCTGGGG67850.022.5091744
CATGGGG112100.022.0917974
ATAACGC8050.021.0155243
Produced by FastQC (version 0.11.5)