FastQCFastQC Report
Mon 6 Jun 2016
ERR1211635_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211635_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4167766
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143970.34543685993887374No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124200.29800137531713633No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83010.1991714506044725No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54600.13100543552589083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68950.045.955051
TACCTGG39600.039.5593872
GTACCTG42200.038.3248061
GTATCAA110600.036.4509851
TCAACGC134200.029.2699764
ACCTGGG53050.028.9625073
CAACGCA136250.028.7933735
ATCAACG136250.028.760963
AACGCAG140900.027.9401456
TATCAAC143000.027.4037192
GTACATG121250.027.2216551
TACATGG123550.026.0062942
ACATGGG124400.025.3062743
TATAACG3900.024.1249222
ACGCAGA162850.024.1164937
CATGGGG80550.024.0909044
CGCAGAG166500.023.6168798
ATAACGC4700.021.9937173
TAACGCA4200.021.2555794
GCAGAGT183650.020.8742319
Produced by FastQC (version 0.11.5)