FastQCFastQC Report
Mon 6 Jun 2016
ERR1211635_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211635_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4167766
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171740.4120672801688003No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151220.36283227033379517No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111450.2674094466915849No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56990.13673992253883735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76100.047.814421
GTACCTG43850.042.1349681
TACCTGG44450.041.1550062
GTATCAA127700.038.201081
ACCTGGG53700.033.253413
TAACGCA5000.031.9543934
TATAACG5250.029.559662
TCAACGC165700.028.9267254
ATCAACG166650.028.7621733
ATAACGC5600.028.531053
CAACGCA168500.028.4732515
TATCAAC171300.028.0568122
AACGCAG175350.027.3890656
GTACATG129300.024.2044811
ACGCAGA199250.023.9383687
CCTGGGG50150.023.8941124
CGCAGAG203550.023.363418
TACATGG132500.023.3181612
TATCACG4650.022.2492072
ACATGGG133750.022.2049663
Produced by FastQC (version 0.11.5)