##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211635_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4167766 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.73333435706323 31.0 31.0 34.0 30.0 34.0 2 32.0179794163108 33.0 31.0 34.0 30.0 34.0 3 32.09690947140506 33.0 31.0 34.0 30.0 34.0 4 35.83156420010145 37.0 35.0 37.0 35.0 37.0 5 35.70025476478286 37.0 35.0 37.0 33.0 37.0 6 35.73271460057978 37.0 35.0 37.0 33.0 37.0 7 35.61882840831275 37.0 35.0 37.0 33.0 37.0 8 35.597248981828635 37.0 35.0 37.0 33.0 37.0 9 37.161492271878984 39.0 37.0 39.0 33.0 39.0 10-11 37.102007526334255 39.0 37.0 39.0 33.0 39.0 12-13 36.9876444598857 39.0 37.0 39.0 33.0 39.0 14-15 38.10405514609026 40.0 37.0 41.0 33.0 41.0 16-17 38.02369638794501 40.0 37.0 41.0 33.0 41.0 18-19 38.00391024832008 40.0 37.0 41.0 33.0 41.0 20-21 37.92822857617246 40.0 37.0 41.0 33.0 41.0 22-23 37.8076050814753 39.5 37.0 41.0 32.5 41.0 24-25 37.675715239291264 39.0 37.0 41.0 32.0 41.0 26-27 37.4383970933109 39.0 36.0 41.0 32.0 41.0 28-29 37.4317638034381 39.0 36.0 41.0 32.0 41.0 30-31 37.28508654276656 39.0 36.0 40.5 31.5 41.0 32-33 37.06298530195794 39.0 36.0 40.0 31.0 41.0 34-35 36.89682566151747 39.0 35.5 40.0 30.5 41.0 36-37 36.78056841482943 39.0 35.0 40.0 30.0 41.0 38-39 36.68082433130843 39.0 35.0 40.0 30.0 41.0 40-41 36.495144401101214 39.0 35.0 40.0 30.0 41.0 42-43 36.27727612346759 38.0 35.0 40.0 29.5 41.0 44-45 36.043412825960004 38.0 35.0 40.0 28.5 41.0 46-47 35.755485312755084 38.0 34.5 40.0 27.5 41.0 48-49 35.470387133058814 38.0 34.0 40.0 26.5 41.0 50-51 35.23346296313181 38.0 34.0 40.0 26.0 41.0 52-53 34.99723724892424 38.0 33.5 40.0 25.5 41.0 54-55 34.75509085682833 38.0 33.0 40.0 24.5 41.0 56-57 34.52384310923406 37.5 33.0 40.0 24.0 41.0 58-59 34.224651407972516 37.0 32.5 40.0 23.5 41.0 60-61 33.87225386454038 36.5 32.0 39.5 23.0 41.0 62-63 33.50006202363568 36.0 31.0 39.0 22.0 40.0 64-65 33.15025639635239 36.0 31.0 39.0 22.0 40.0 66-67 32.759924261582825 35.0 31.0 38.5 21.5 40.0 68-69 32.342332678945986 35.0 30.0 38.0 20.0 40.0 70-71 31.949923652143617 34.5 30.0 37.5 20.0 40.0 72-73 31.50326373889513 34.0 29.0 37.0 19.0 39.0 74-75 31.100086713121613 34.0 29.0 36.0 18.0 39.0 76-77 29.74475210460472 32.5 27.0 35.0 18.0 37.0 78-79 30.66951911407694 34.0 29.0 35.0 19.0 38.0 80-81 30.652175649976513 34.0 29.0 35.0 18.5 37.0 82-83 30.35898092647236 34.0 29.0 35.0 18.0 37.0 84-85 30.012693730886042 34.0 29.0 35.0 18.0 36.0 86-87 29.693161636233896 33.0 28.0 35.0 17.0 36.0 88-89 29.37771038489205 33.0 27.0 35.0 15.5 35.5 90-91 29.0565227270437 33.0 26.5 35.0 14.0 35.0 92-93 28.792560690787344 33.0 26.0 35.0 11.5 35.0 94-95 28.538180766386596 32.0 25.5 34.0 11.0 35.0 96-97 28.262169949080636 32.0 25.0 34.0 10.0 35.0 98-99 28.008472524609104 32.0 25.0 34.0 10.0 35.0 100 26.211967514490976 30.0 20.0 34.0 8.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1636804106882188 1101 2 0.0823941038222813 1101 3 0.09135720225032884 1101 4 0.049867268773823525 1101 5 0.07539961273943874 1101 6 0.03263354464448298 1101 7 0.11758115356843746 1101 8 0.080493538504669 1101 9 0.0800027044074767 1101 10-11 0.0906401420929015 1101 12-13 0.06949066590164676 1101 14-15 0.07785757024696949 1101 16-17 0.03113331945922937 1101 18-19 0.02169065720828911 1101 20-21 -0.07525508936532077 1101 22-23 -0.010834887121724535 1101 24-25 -0.11355818489351321 1101 26-27 -0.19958947507144842 1101 28-29 0.004461688116379037 1101 30-31 0.0094908924020487 1101 32-33 -0.0754547487894186 1101 34-35 -0.22882857135909518 1101 36-37 -0.1906054683064511 1101 38-39 -0.29158716515411953 1101 40-41 -0.19703476656172825 1101 42-43 -0.24060304713982106 1101 44-45 -0.3085137093931465 1101 46-47 -0.4073003605835126 1101 48-49 -0.3922280446180366 1101 50-51 -0.3803927954466104 1101 52-53 -0.5276031890199491 1101 54-55 -0.6797684996953919 1101 56-57 -0.6922624917922136 1101 58-59 -0.6635047628274506 1101 60-61 -0.7531595932856447 1101 62-63 -0.8391269849200214 1101 64-65 -0.8633391743613643 1101 66-67 -0.9276745835055245 1101 68-69 -0.9532030169980779 1101 70-71 -0.9667167843629407 1101 72-73 -1.120038322660509 1101 74-75 -1.1803714978808522 1101 76-77 -0.9444501781699728 1101 78-79 -0.8635032986580065 1101 80-81 -0.8362033195078311 1101 82-83 -0.9767275050121391 1101 84-85 -1.0105971034394976 1101 86-87 -1.1350340260227476 1101 88-89 -1.1083718556118143 1101 90-91 -1.3450370408265542 1101 92-93 -1.4300021349904135 1101 94-95 -1.5231468117987674 1101 96-97 -1.695722363866139 1101 98-99 -1.6978765912916174 1101 100 -1.5584587139555026 1102 1 0.17737611446225188 1102 2 0.1890613692362848 1102 3 0.1671162748762427 1102 4 0.1414204407447528 1102 5 0.09036423318708842 1102 6 0.052032958299776055 1102 7 0.07287269006101837 1102 8 0.08772084651526768 1102 9 0.08348228177739259 1102 10-11 0.13788547667505924 1102 12-13 0.16186626010492233 1102 14-15 0.10385432418105722 1102 16-17 0.08151277558281578 1102 18-19 0.02373869385161953 1102 20-21 -0.04444504837250918 1102 22-23 0.04800294430096841 1102 24-25 0.04411946664836108 1102 26-27 0.07490164315335335 1102 28-29 0.020229164588819515 1102 30-31 0.06701425958598861 1102 32-33 0.16616770680170134 1102 34-35 0.012491730666170042 1102 36-37 -0.023502678357708362 1102 38-39 0.04299498404944302 1102 40-41 -0.0542486027572906 1102 42-43 -0.03520694370194377 1102 44-45 -0.14571837192418258 1102 46-47 -0.22787984108466475 1102 48-49 -0.216995014293623 1102 50-51 -0.3220986479879784 1102 52-53 -0.36208654491322534 1102 54-55 -0.39048308325592274 1102 56-57 -0.3166766282168041 1102 58-59 -0.24824632850896222 1102 60-61 -0.2842798067818322 1102 62-63 -0.39739066041607174 1102 64-65 -0.4091016470167901 1102 66-67 -0.503441675068963 1102 68-69 -0.45424515811854604 1102 70-71 -0.4199804919347443 1102 72-73 -0.6109426022070856 1102 74-75 -0.690324282376718 1102 76-77 -0.4248996877317559 1102 78-79 -0.5247772833001356 1102 80-81 -0.5155693102315233 1102 82-83 -0.7051161476305445 1102 84-85 -0.7100563062929552 1102 86-87 -0.637389348351963 1102 88-89 -0.9201183160527933 1102 90-91 -1.0469125099344012 1102 92-93 -0.9888418909581098 1102 94-95 -1.053786209801089 1102 96-97 -1.0698840641375007 1102 98-99 -1.142699244469366 1102 100 -1.0619607443504826 1103 1 0.18196721919683156 1103 2 0.13663791408686166 1103 3 0.10300472625966961 1103 4 0.11042026096408364 1103 5 0.11170419842949286 1103 6 0.07917102632736572 1103 7 0.034603378620971625 1103 8 0.008785735585895793 1103 9 0.1099378882903892 1103 10-11 0.0703625017632703 1103 12-13 0.10053702295072497 1103 14-15 0.15471545007805076 1103 16-17 0.17155510041710187 1103 18-19 0.07832180833430158 1103 20-21 0.05722544303972654 1103 22-23 0.07347845241636008 1103 24-25 -0.0748714232894443 1103 26-27 -0.03084422923527086 1103 28-29 -0.0010692043422650954 1103 30-31 0.1678355517272223 1103 32-33 0.22354981827276532 1103 34-35 0.08906125702048939 1103 36-37 0.015519949330624172 1103 38-39 0.14644935794857616 1103 40-41 0.10046288452235075 1103 42-43 0.0980508347495217 1103 44-45 0.06766289117013713 1103 46-47 0.04883697616299543 1103 48-49 0.02383940301138665 1103 50-51 0.05728127321403775 1103 52-53 0.007098872407055978 1103 54-55 0.009784531430632626 1103 56-57 -0.06046526490356996 1103 58-59 -0.17691194469251315 1103 60-61 -0.14576867271834004 1103 62-63 -0.18605860317296674 1103 64-65 -0.18540602626234914 1103 66-67 -0.23829156996189482 1103 68-69 -0.28881298536930444 1103 70-71 -0.22678454393248515 1103 72-73 -0.4167359460922526 1103 74-75 -0.35151245633390005 1103 76-77 -0.14413851048564297 1103 78-79 -0.055459733064367356 1103 80-81 -0.16297858855902447 1103 82-83 -0.17223872866991385 1103 84-85 -0.15906434002121728 1103 86-87 -0.2809752911488097 1103 88-89 -0.24563771180174498 1103 90-91 -0.3145394168726412 1103 92-93 -0.36263351223896834 1103 94-95 -0.4481413318463119 1103 96-97 -0.384375502230089 1103 98-99 -0.5171925978723273 1103 100 -0.5305081753082455 1104 1 0.2277742942119687 1104 2 0.0879189609266362 1104 3 0.15835995417452864 1104 4 0.14613989802393945 1104 5 0.10614355579235024 1104 6 0.08686089824716703 1104 7 0.1517209457010793 1104 8 0.17924600493462606 1104 9 0.1805246545876784 1104 10-11 0.20760075509340226 1104 12-13 0.20025783194359548 1104 14-15 0.13399998333695606 1104 16-17 0.12561834562694685 1104 18-19 0.15277562715476734 1104 20-21 0.05710490368938537 1104 22-23 0.08474729026033145 1104 24-25 0.06323419105319061 1104 26-27 0.11139399961157892 1104 28-29 0.11208541736657907 1104 30-31 0.2759159319688891 1104 32-33 0.09640071672754402 1104 34-35 0.07780229409960526 1104 36-37 0.24403064770804406 1104 38-39 0.11701784339653187 1104 40-41 0.13410691068354907 1104 42-43 0.023127018556941437 1104 44-45 0.1060259880113037 1104 46-47 0.2248874313071738 1104 48-49 0.209090022030324 1104 50-51 -0.01686193438948891 1104 52-53 -0.025537585156079956 1104 54-55 -0.1839878197640772 1104 56-57 -0.07230970466898867 1104 58-59 -0.2354961908441524 1104 60-61 -0.12363782753917718 1104 62-63 -0.2295010829701809 1104 64-65 -0.1861120424572178 1104 66-67 -0.1576423386552861 1104 68-69 -0.20333475345387342 1104 70-71 -0.2172190895146393 1104 72-73 -0.235909129392045 1104 74-75 -0.21122040344348747 1104 76-77 -0.13188397577406263 1104 78-79 -0.04797135854199652 1104 80-81 0.0449482815147384 1104 82-83 0.006374881095975837 1104 84-85 -0.3429544231501147 1104 86-87 -0.337284387666795 1104 88-89 -0.3932862559442789 1104 90-91 -0.16175305634491366 1104 92-93 -0.35105520573115 1104 94-95 -0.32406039338585657 1104 96-97 -0.39711374495080065 1104 98-99 -0.2994797606355455 1104 100 -0.305163736358935 1105 1 0.27232328947373574 1105 2 0.06747631485379912 1105 3 0.08539233270213487 1105 4 0.11811153447764866 1105 5 0.08335348083871708 1105 6 0.067215134253658 1105 7 0.08805576890969036 1105 8 0.07841437129239637 1105 9 0.08650291630927853 1105 10-11 0.12522423941575056 1105 12-13 0.16644743542564555 1105 14-15 0.17502686850472315 1105 16-17 0.18503045369855897 1105 18-19 0.12922812490375435 1105 20-21 0.12957150168905684 1105 22-23 0.1805103170012714 1105 24-25 0.020540876992605206 1105 26-27 0.12753160639424976 1105 28-29 0.10464288678389266 1105 30-31 0.2141167573315741 1105 32-33 0.21004371000245925 1105 34-35 0.16050158124836855 1105 36-37 0.20547951091386807 1105 38-39 0.03719473146462349 1105 40-41 0.02895275745371606 1105 42-43 0.08222338385700567 1105 44-45 0.13610861311823186 1105 46-47 0.11370105998176427 1105 48-49 0.1247517936911251 1105 50-51 0.1618466975329298 1105 52-53 0.12633766666210988 1105 54-55 0.009762673420702583 1105 56-57 -0.10900806058038626 1105 58-59 -0.008477085845832733 1105 60-61 -0.051423295951046555 1105 62-63 0.009157809797990524 1105 64-65 -0.03701644534960025 1105 66-67 0.018356011037404585 1105 68-69 -0.08928148270471326 1105 70-71 -0.10733590179819075 1105 72-73 -0.027770078198550152 1105 74-75 -0.152504502434752 1105 76-77 -0.04343871366627994 1105 78-79 -0.009120861855937079 1105 80-81 0.006958761331919305 1105 82-83 0.029766628409785056 1105 84-85 -0.042114375971603835 1105 86-87 -0.006149809234610615 1105 88-89 -0.09543633252836514 1105 90-91 -0.11983655729574849 1105 92-93 -0.21280635527627112 1105 94-95 -0.12989612144154705 1105 96-97 -0.12671954203407054 1105 98-99 -0.02676574718538305 1105 100 -0.051904640725247475 1106 1 0.19671616945766957 1106 2 0.20639258262615812 1106 3 0.16633131776681154 1106 4 0.10333904212256328 1106 5 0.14825603049226999 1106 6 0.1542992108868333 1106 7 0.11048475842468974 1106 8 0.09198186530424124 1106 9 0.16890804584659946 1106 10-11 0.14695105003944064 1106 12-13 0.11785399310915778 1106 14-15 0.09256095223232563 1106 16-17 0.1297826269690745 1106 18-19 0.11528596356112786 1106 20-21 0.13561928185222172 1106 22-23 0.14273570305756067 1106 24-25 0.17019308213536277 1106 26-27 0.12499003668812492 1106 28-29 0.165065743522554 1106 30-31 0.27338535028338384 1106 32-33 0.27496731133916086 1106 34-35 0.26110220682018337 1106 36-37 0.24139320869674918 1106 38-39 0.20799756266338676 1106 40-41 0.3657523205891877 1106 42-43 0.3700395222457189 1106 44-45 0.2985148226068972 1106 46-47 0.1997188162344301 1106 48-49 0.2123290060019869 1106 50-51 0.2275520004940219 1106 52-53 0.18028002589056058 1106 54-55 0.12125507144740055 1106 56-57 0.17136457105262792 1106 58-59 0.16246426542802794 1106 60-61 0.08010277845317404 1106 62-63 0.08180285273711263 1106 64-65 0.09565020924743806 1106 66-67 0.060591108235136915 1106 68-69 0.1695399093420491 1106 70-71 0.2534800530198389 1106 72-73 0.14163865791312702 1106 74-75 0.19123885312775712 1106 76-77 0.18436097072688895 1106 78-79 0.2452274685561271 1106 80-81 0.33556274633133043 1106 82-83 0.3210742937065483 1106 84-85 0.28142846862445126 1106 86-87 0.14413263067230986 1106 88-89 0.2362100417191968 1106 90-91 0.24045200023029167 1106 92-93 0.19137901405656166 1106 94-95 0.2541578917039615 1106 96-97 0.28134854710885904 1106 98-99 0.17691133978375362 1106 100 0.20554691829925176 1107 1 0.25305179013792767 1107 2 0.14436439953931313 1107 3 0.11234559510555897 1107 4 0.10090037522468975 1107 5 0.015159482982340933 1107 6 0.07385933012614743 1107 7 0.09070643289476266 1107 8 0.06796245175686977 1107 9 0.05170505000015879 1107 10-11 0.1616467379363158 1107 12-13 0.17851245175111075 1107 14-15 0.23982893515567127 1107 16-17 0.19086985993989458 1107 18-19 0.15072477083769797 1107 20-21 0.12130024237277581 1107 22-23 0.13654717034519592 1107 24-25 0.083380318409219 1107 26-27 0.08640860466031342 1107 28-29 0.15549738735703755 1107 30-31 0.23951630708803862 1107 32-33 0.2129736036076011 1107 34-35 0.16216413033857435 1107 36-37 0.24792443056939817 1107 38-39 0.24041513146958948 1107 40-41 0.1824938214851386 1107 42-43 0.15387987038487694 1107 44-45 0.3191874429816295 1107 46-47 0.37403457332974455 1107 48-49 0.33871751641890313 1107 50-51 0.2641319007425764 1107 52-53 0.18018413485744844 1107 54-55 0.2608785056056604 1107 56-57 0.2316444201042458 1107 58-59 0.08695023168759519 1107 60-61 0.17662314162111414 1107 62-63 0.18465274662732867 1107 64-65 0.30949990908477076 1107 66-67 0.3012163920891098 1107 68-69 0.27129742992404005 1107 70-71 0.21547200601775174 1107 72-73 0.16639155012706297 1107 74-75 0.2102490181965848 1107 76-77 0.2512264383943723 1107 78-79 0.38781485429199947 1107 80-81 0.4746026799404639 1107 82-83 0.41161194409754387 1107 84-85 0.28858210484545666 1107 86-87 0.25830065983804573 1107 88-89 0.15137021793574235 1107 90-91 0.13745481193291909 1107 92-93 0.14035548823927257 1107 94-95 0.176568008053124 1107 96-97 0.3066720374186005 1107 98-99 0.34406365479353695 1107 100 0.3776584202686806 1108 1 0.2720911879916663 1108 2 0.1297818942398976 1108 3 0.13005144433714833 1108 4 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2316 84-85 0.22836549422562058 2316 86-87 0.36881203526877826 2316 88-89 0.35024876237880065 2316 90-91 0.3265646014459378 2316 92-93 0.3905090980703001 2316 94-95 0.424040192218861 2316 96-97 0.5218212880517221 2316 98-99 0.47738505222783445 2316 100 0.24143037194385997 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 12.0 11 39.0 12 85.0 13 213.0 14 707.0 15 1874.0 16 4168.0 17 8117.0 18 14192.0 19 21473.0 20 28781.0 21 38097.0 22 47659.0 23 53829.0 24 58442.0 25 65217.0 26 76159.0 27 89441.0 28 105409.0 29 125339.0 30 148063.0 31 175353.0 32 209788.0 33 259879.0 34 329839.0 35 437932.0 36 586315.0 37 736189.0 38 499135.0 39 46018.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.75519226181773 20.51722545843497 13.830288642817019 23.897293636930275 2 15.061926696366513 21.89130625293367 39.02040933924449 24.02635771145533 3 17.080202124902826 27.218791089612548 31.10712811801177 24.59387866747286 4 11.924196424950914 17.819171590023224 37.631615759123875 32.62501622590199 5 12.24219009245034 38.26929679820124 35.35671876750794 14.131794341840479 6 30.84962675179042 38.02117024686784 17.709231102929742 13.419971898411992 7 26.736265903603996 33.00442011379718 22.54454784649618 17.71476613610265 8 26.13025803174524 36.238966750109114 19.957156667289706 17.673618550855945 9 26.386894081865442 14.958901243495918 20.182491051560955 38.47171362307768 10-11 24.763137010730592 26.36991235694495 28.95116858406469 19.915782048259768 12-13 25.16338120698024 24.184149701502527 29.02566087299323 21.626808218523998 14-15 22.36222263464679 25.53920080196451 26.729346936625873 25.36922962676283 16-17 21.95286629919518 28.396565925925216 27.338467824810493 22.312099950069115 18-19 21.845019928778743 27.360618218633633 29.476537091714373 21.31782476087325 20-21 22.516501550190156 26.62137725604765 29.50665166723872 21.35546952652347 22-23 22.22822730450798 26.50645453703495 29.432050647757098 21.833267510699976 24-25 21.99616317184832 26.616792619450397 29.546803916871045 21.840240291830238 26-27 22.040559202132464 26.722403248076095 29.433992052564456 21.803045497226986 28-29 22.051907433523105 26.829090093294695 29.520893680758547 21.598108792423652 30-31 22.344005104963554 26.740953691938866 29.474422215469243 21.440618987628334 32-33 22.119150460577693 26.84263168673255 29.38774917690356 21.650468675786204 34-35 22.333979667468647 26.93572114645167 29.30539035950367 21.42490882657602 36-37 22.407056361175666 26.914925868593485 29.166575189364014 21.511442580866834 38-39 22.30083029965074 27.058449217928942 29.107504657298644 21.533215825121676 40-41 22.362500920807662 26.929335000505095 29.14965122085299 21.558512857834256 42-43 22.200220190953743 27.04336533173101 29.049116308624402 21.70729816869084 44-45 22.202515234456033 27.221190348185804 28.900168916466672 21.676125500891494 46-47 22.206961322016273 27.093496768936294 28.679970524387087 22.019571384660345 48-49 22.234811674117157 27.24077446724783 28.651988837459296 21.872425021175715 50-51 22.26236152002246 27.4871687818081 28.405569672495 21.844900025674434 52-53 22.320383315349527 27.507204918660182 28.25119930013132 21.921212465858968 54-55 22.22067055024568 27.477647225376558 28.3795085718879 21.922173652489864 56-57 22.300481762466486 27.513716481420786 28.19510156170893 21.9907001944038 58-59 22.27487736594233 27.471024674615446 28.338700822807105 21.91539713663512 60-61 22.424111976227223 27.551652614928628 28.319339184384912 21.70489622445924 62-63 22.280556200444764 27.589440134184944 28.280605391993657 21.849398273376632 64-65 22.23890687528082 27.381747221507364 28.299887042659243 22.079458860552574 66-67 22.159636940310772 27.495309503202066 28.20950797714392 22.13554557934324 68-69 22.13602586562607 27.46726572174631 28.244136782003487 22.152571630624134 70-71 22.093455436830492 27.379840061886757 28.227903653219155 22.29880084806359 72-73 22.061392736685004 27.61607708808938 28.027532077543544 22.294998097682075 74-75 22.106413424888842 27.690566913344085 27.874852112926995 22.328167548840078 76-77 22.01588135497445 27.576911028828043 28.040068226456455 22.36713938974105 78-79 22.009436417448985 27.537322539234516 28.119398529475813 22.333842513840683 80-81 21.989821070295708 27.706983788121047 27.83476929562963 22.468425845953615 82-83 22.09542906315496 27.7101617808613 27.889477021012937 22.304932134970805 84-85 22.079903679098 27.772774730018412 27.80261702662012 22.34470456426347 86-87 21.960638637660022 27.70132725152699 27.902602309521807 22.435431801291184 88-89 21.86842822959302 27.60129472763362 27.964031557336284 22.566245485437072 90-91 22.039032485871466 27.73129300884462 27.824953214897075 22.40472129038684 92-93 21.885568751443664 27.67843097257262 27.83137394893701 22.604626327046702 94-95 21.756069522310113 27.633414292297058 27.882087109285003 22.728429076107826 96-97 21.76183042704605 27.637466208635953 27.881291972983707 22.719411391334294 98-99 21.754153822082653 27.565869138586162 27.728199243315416 22.95177779601577 100 20.80355818698336 28.20514481892055 28.03022558318011 22.961071410915977 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2009.0 1 1557.5 2 2740.5 3 3930.0 4 2454.5 5 1643.5 6 2516.0 7 4759.0 8 7281.5 9 8246.0 10 6749.5 11 5113.5 12 5129.0 13 5341.0 14 5700.5 15 6171.0 16 6571.5 17 6933.0 18 7431.0 19 7984.0 20 8706.5 21 9708.0 22 11335.5 23 13970.5 24 17183.5 25 21092.0 26 25852.0 27 31335.0 28 37425.5 29 44291.5 30 51625.0 31 58900.5 32 66346.0 33 74106.5 34 81846.0 35 89315.0 36 98589.0 37 107842.5 38 114724.0 39 120959.5 40 126357.5 41 130630.0 42 135074.5 43 140567.0 44 145195.0 45 149333.5 46 153484.5 47 157019.0 48 160805.5 49 162223.0 50 160174.0 51 155360.5 52 148454.0 53 141460.5 54 133809.0 55 124013.5 56 112268.0 57 100275.5 58 88419.0 59 75739.0 60 62590.0 61 50994.5 62 41257.0 63 31948.0 64 23719.5 65 17686.5 66 13402.5 67 10153.0 68 7589.5 69 5657.0 70 4323.5 71 3353.5 72 2567.0 73 1967.5 74 1541.0 75 1174.0 76 863.5 77 577.0 78 383.5 79 284.0 80 202.5 81 127.0 82 89.5 83 69.0 84 49.5 85 35.5 86 25.0 87 20.0 88 16.5 89 7.5 90 4.5 91 3.0 92 3.0 93 3.5 94 2.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22242131635989162 2 0.06624652151776275 3 0.0011037087974708753 4 0.0011277024669811117 5 3.5990504265354627E-4 6 7.198100853070925E-4 7 0.0 8 5.518543987354376E-4 9 0.0 10-11 2.039461908370096E-4 12-13 0.0014156265011039486 14-15 1.199683475511821E-5 16-17 1.1996834755118209E-4 18-19 0.0011876866407567028 20-21 0.0012356739797771755 22-23 0.0 24-25 1.199683475511821E-5 26-27 1.5595885181653673E-4 28-29 0.0014036296663488307 30-31 0.0013796359968385942 32-33 0.002867243506473252 34-35 0.0038149934521275906 36-37 0.0015715853529204855 38-39 0.002735278324166952 40-41 0.00549455031784414 42-43 0.011372999347852063 44-45 0.0077139647475410085 46-47 0.006070398386089814 48-49 0.006142379394620524 50-51 0.004702759224006339 52-53 0.003047196027800025 54-55 0.005242616787986657 56-57 0.003647037765555936 58-59 0.0019674808998393863 60-61 0.0074380375481732895 62-63 0.009201572257175666 64-65 0.006322331915947297 66-67 0.007126119844540217 68-69 0.01287260369224184 70-71 0.003695025104576408 72-73 0.011552951869178835 74-75 0.0074980217219488806 76-77 0.004186895329536255 78-79 0.014696122575019809 80-81 0.005650509169660677 82-83 0.012572682823363884 84-85 0.020670546283068678 86-87 0.013988309324467832 88-89 0.013376470751956802 90-91 0.026596982652097072 92-93 0.020478596926986784 94-95 0.025097378307707294 96-97 0.015619878851163909 98-99 0.015355948486551307 100 0.016795568657165492 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4167766.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.71617590555961 #Duplication Level Percentage of deduplicated Percentage of total 1 77.37603745329108 51.62233325608937 2 14.720353526301611 19.641713905055262 3 4.1580803436550875 8.322336589102276 4 1.544197672297255 4.120918541517575 5 0.7240912874757717 2.4154300853458364 6 0.3935234673765405 1.5752628523475414 7 0.24853849055120938 1.1607076358441726 8 0.17562487641649718 0.9373616118716155 9 0.11490365127838154 0.6899338989791625 >10 0.5160302646994237 5.736309521406544 >50 0.019997266214518118 0.9044537679003277 >100 0.007797823942574482 0.9114189560858496 >500 4.3361921209140113E-4 0.20956468648380683 >1k 2.1680960447014827E-4 0.3306632182470616 >5k 4.336192089402964E-5 0.1648687384768157 >10k+ 1.3008576268208892E-4 1.2567227352467716 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17174 0.4120672801688003 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15122 0.36283227033379517 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11145 0.2674094466915849 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5699 0.13673992253883735 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 2.399366951023642E-5 5 0.0 0.0 0.0 0.0 2.399366951023642E-5 6 0.0 0.0 0.0 0.0 4.798733902047284E-5 7 0.0 0.0 0.0 0.0 4.798733902047284E-5 8 0.0 0.0 0.0 0.0 4.798733902047284E-5 9 0.0 0.0 0.0 0.0 4.798733902047284E-5 10-11 0.0 0.0 0.0 0.0 4.798733902047284E-5 12-13 0.0 0.0 0.0 0.0 4.798733902047284E-5 14-15 0.0 0.0 0.0 0.0 4.798733902047284E-5 16-17 2.399366951023642E-5 0.0 0.0 0.0 4.798733902047284E-5 18-19 2.399366951023642E-5 1.199683475511821E-5 0.0 4.798733902047284E-5 7.198100853070926E-5 20-21 2.399366951023642E-5 2.399366951023642E-5 0.0 7.198100853070926E-5 7.198100853070926E-5 22-23 2.399366951023642E-5 2.399366951023642E-5 0.0 1.5595885181653673E-4 8.397784328582748E-5 24-25 2.399366951023642E-5 2.399366951023642E-5 0.0 3.1191770363307345E-4 9.597467804094568E-5 26-27 2.399366951023642E-5 2.399366951023642E-5 0.0 3.719018774086645E-4 9.597467804094568E-5 28-29 2.399366951023642E-5 2.399366951023642E-5 0.0 0.0012956581535527667 9.597467804094568E-5 30-31 2.399366951023642E-5 2.399366951023642E-5 0.0 0.003934961799678773 9.597467804094568E-5 32-33 2.399366951023642E-5 2.399366951023642E-5 0.0 0.00998136651625835 9.597467804094568E-5 34-35 2.399366951023642E-5 2.399366951023642E-5 0.0 0.018835030565535586 9.597467804094568E-5 36-37 2.399366951023642E-5 2.399366951023642E-5 0.0 0.03152768173645065 9.597467804094568E-5 38-39 2.399366951023642E-5 2.399366951023642E-5 0.0 0.05287005076580595 9.597467804094568E-5 40-41 2.399366951023642E-5 2.399366951023642E-5 0.0 0.07784746072596206 9.597467804094568E-5 42-43 2.399366951023642E-5 2.399366951023642E-5 0.0 0.10256094032150558 9.597467804094568E-5 44-45 3.599050426535463E-5 2.399366951023642E-5 0.0 0.1314373215770751 1.0797151279606389E-4 46-47 4.798733902047284E-5 4.798733902047284E-5 0.0 0.16709191446928642 1.199683475511821E-4 48-49 4.798733902047284E-5 7.198100853070926E-5 0.0 0.2044260642272143 1.199683475511821E-4 50-51 9.597467804094568E-5 7.198100853070926E-5 0.0 0.24353574552889964 1.199683475511821E-4 52-53 9.597467804094568E-5 7.198100853070926E-5 0.0 0.28389309764511733 1.6795568657165493E-4 54-55 9.597467804094568E-5 7.198100853070926E-5 0.0 0.329433082375546 2.27939860347246E-4 56-57 9.597467804094568E-5 8.397784328582748E-5 0.0 0.38079153196220705 2.399366951023642E-4 58-59 9.597467804094568E-5 1.199683475511821E-4 0.0 0.4380404274136312 2.399366951023642E-4 60-61 9.597467804094568E-5 1.199683475511821E-4 0.0 0.4970168670697923 2.399366951023642E-4 62-63 9.597467804094568E-5 1.199683475511821E-4 1.199683475511821E-5 0.5599402653603873 2.399366951023642E-4 64-65 9.597467804094568E-5 1.199683475511821E-4 2.399366951023642E-5 0.6231875781893705 2.399366951023642E-4 66-67 9.597467804094568E-5 1.199683475511821E-4 2.399366951023642E-5 0.6941248620963845 2.759271993677188E-4 68-69 9.597467804094568E-5 1.199683475511821E-4 2.399366951023642E-5 0.7768070472286592 3.1191770363307345E-4 70-71 9.597467804094568E-5 1.199683475511821E-4 2.399366951023642E-5 0.8616726562863654 3.3591137314330986E-4 72-73 1.199683475511821E-4 1.199683475511821E-4 2.399366951023642E-5 0.9452426071905189 4.0789238167401914E-4 74-75 1.199683475511821E-4 1.199683475511821E-4 2.399366951023642E-5 1.0300602289092047 4.0789238167401914E-4 76-77 1.199683475511821E-4 1.199683475511821E-4 2.399366951023642E-5 1.1213921318999196 4.0789238167401914E-4 78-79 1.199683475511821E-4 1.199683475511821E-4 2.399366951023642E-5 1.2253447050530188 4.0789238167401914E-4 80-81 1.199683475511821E-4 1.199683475511821E-4 2.399366951023642E-5 1.3354996417745142 4.0789238167401914E-4 82-83 1.3196518230630032E-4 1.199683475511821E-4 2.399366951023642E-5 1.4521928534375492 4.0789238167401914E-4 84-85 1.4396201706141852E-4 1.199683475511821E-4 2.399366951023642E-5 1.5667986158531932 4.0789238167401914E-4 86-87 1.4396201706141852E-4 1.199683475511821E-4 2.399366951023642E-5 1.6859271849715172 4.0789238167401914E-4 88 1.4396201706141852E-4 1.199683475511821E-4 2.399366951023642E-5 1.7820098345252589 4.0789238167401914E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7610 0.0 47.81442 1 GTACCTG 4385 0.0 42.134968 1 TACCTGG 4445 0.0 41.155006 2 GTATCAA 12770 0.0 38.20108 1 ACCTGGG 5370 0.0 33.25341 3 TAACGCA 500 0.0 31.954393 4 TATAACG 525 0.0 29.55966 2 TCAACGC 16570 0.0 28.926725 4 ATCAACG 16665 0.0 28.762173 3 ATAACGC 560 0.0 28.53105 3 CAACGCA 16850 0.0 28.473251 5 TATCAAC 17130 0.0 28.056812 2 AACGCAG 17535 0.0 27.389065 6 GTACATG 12930 0.0 24.204481 1 ACGCAGA 19925 0.0 23.938368 7 CCTGGGG 5015 0.0 23.894112 4 CGCAGAG 20355 0.0 23.36341 8 TACATGG 13250 0.0 23.318161 2 TATCACG 465 0.0 22.249207 2 ACATGGG 13375 0.0 22.204966 3 >>END_MODULE