FastQCFastQC Report
Mon 6 Jun 2016
ERR1211634_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211634_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7195019
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226060.3141895803193848No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191890.26669839231835246No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121990.16954784969991044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115900.047.6393661
GTATCAA194950.036.5454861
TACCTGG62750.031.7854212
GTACCTG63350.031.7814561
TCAACGC228400.029.809164
ATCAACG229450.029.7348023
CAACGCA230700.029.530085
GTACATG225500.028.8970851
AACGCAG240700.028.436916
TATCAAC246900.027.893042
TACATGG231650.027.3330822
ACATGGG227700.026.6615873
ACGCAGA270350.025.0752327
CGCAGAG275450.024.5441048
ACCTGGG79750.024.1568153
CATGGGG123200.023.7985954
GCAGAGT311800.020.929189
GAGTACT203400.020.39812312-13
GTATAGG27600.019.304651
TATAACG7100.019.2138982
Produced by FastQC (version 0.11.5)