FastQCFastQC Report
Mon 6 Jun 2016
ERR1211634_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211634_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7195019
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247790.34439102940520383No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219290.30478029314446564No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143950.20006896437660557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128750.044.047041
GTATCAA208000.035.2650571
TACCTGG61900.029.933922
GTACCTG66100.029.0975911
TCAACGC252700.028.2836044
ATCAACG253000.028.232083
CAACGCA257050.027.8957865
GTACATG248700.027.0487081
AACGCAG267550.026.8890196
TATCAAC269200.026.571262
TACATGG253650.025.95682
ACATGGG245300.025.7083853
ACGCAGA302400.023.696977
CGCAGAG305500.023.3488398
CATGGGG131450.022.9143854
ACCTGGG78050.022.8184493
GCAGAGT345750.020.2906679
TAACGCA7350.019.8191554
GAGTACT216400.019.79217712-13
ATGGGGG75200.019.4331995
Produced by FastQC (version 0.11.5)