FastQCFastQC Report
Mon 6 Jun 2016
ERR1211633_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211633_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6729384
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185340.2754189685118281No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163540.2430237299580467No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104430.1551850808335503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97200.047.418721
GTATCAA165050.036.7861671
TCAACGC193400.030.2728044
GTACCTG59950.029.903081
CAACGCA196600.029.7524035
ATCAACG197600.029.6068953
AACGCAG206550.028.611256
TACCTGG59050.028.44642
GTACATG202250.028.2006631
TATCAAC211800.027.7469652
TACATGG210250.026.6311532
ACATGGG206400.025.6581133
ACGCAGA236450.024.715477
CGCAGAG241800.023.8595478
CATGGGG116700.023.1922444
ACCTGGG71750.022.6602483
TATAACG6250.021.8321742
GCAGAGT271050.020.6087939
GAGTACT171500.019.87688312-13
TAACGCA7800.019.2773134
Produced by FastQC (version 0.11.5)