FastQCFastQC Report
Mon 6 Jun 2016
ERR1211633_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211633_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6729384
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210280.31248031023344786No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187690.2789111157871211No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129810.1929002714067142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119550.043.5033571
GTATCAA195650.034.2460981
GTACCTG59600.030.2213551
TACCTGG59650.029.6428342
ATCAACG235000.027.912893
TCAACGC235000.027.9124744
CAACGCA238900.027.476685
AACGCAG249150.026.4608256
TATCAAC252250.026.2490632
ACCTGGG71350.024.0373253
GTACATG220650.023.7841761
ACGCAGA284100.023.0400417
TACATGG225400.023.0333732
CGCAGAG291000.022.3806978
ACATGGG224550.022.0972423
CATGGGG122000.020.33554
GCAGAGT323150.019.7902329
GAGTACT199900.019.53336312-13
TATAACG6750.018.8105872
AGAGTAC298900.018.66050110-11
Produced by FastQC (version 0.11.5)